d13C Added Sugar Intake Biomarker: Determining Validity in Children

d13C 添加糖摄入生物标志物：确定儿童的有效性

• 批准号: 8768638
• 负责人: BRENDA M DAVY
• 金额: $ 20.49万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-14 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8768638
• 关键词: Address; Adolescent; Adult; Animals; Applications Grants; Area; Beverages; Biological Markers; Blood; Blood specimen; Calories; Canes; Carbon; Child; Child health care; Clinical; Comorbidity; Consumption; Data; Development; Diet; Dietary Assessment; Dietary intake; Food; Fructose; Funding; Future; Goals; Guidelines; Health; Health Policy; Hour; Human; Intake; Knowledge; Laboratories; Measures; Memory; Methodology; Methods; Molasses; National Health and Nutrition Examination Survey; Nitrogen; Nutritional; Obesity; Outcome; Participant; Pathway interactions; Patient Self-Report; Plants; Preparation; Process; Public Health; Public Policy; Recommendation; Reliance; Reporting; Research; Role; Sampling; Schools; Social Desirability; Source; Staging; Sucrose; Sweetening Agents; Taxation; Techniques; Urine; Validation; Validity and Reliability; Weight; Weight Gain; Work; Youth; aged; base; comparative; feeding; forgetting; health organization; innovation; literacy; minimally invasive; novel; policy implication; public health relevance; stable isotope; sugar; sweetened beverage; urinary

DESCRIPTION (provided by applicant): Consumption of energy-containing added sugars (AS) and in particular, sugar-sweetened beverages (SSB), have been suggested as contributors to weight gain. In children and adolescents, total AS intake represents ~16% of total energy, or ~300-400 kcal/d; SSB comprise ~50% of total AS intake. Although recognized by major health organizations, the role of AS and their primary food source, SSB, in the development and progression of obesity and related co-morbidities remains controversial. A common research limitation in this area is a reliance on self-reported measures of dietary intake, which present additional challenges when studying children. Thus, the need for objective methods to assess dietary intake, such as biomarkers of AS consumption, has been recognized. We have established the validity of the fingerstick blood d13C AS biomarker in adults, and aim to expand our innovative biomarker to studies of diet in children. We propose to establish the validity and reliability of the fingerstick blood d13C AS biomarker in children using two approaches. First, a controlled feeding component (Study 1) will provide data necessary for validation of the biomarker with actual AS intake, and determine its ability to detect levels of AS intake. Second, a cross-sectional component (Study 2) will compare the biomarker to self-reported intake data, collected in a method similar to national nutritional surveillance methodology (i.e., NHANES). Study 1 will include 30 adolescents aged 12-18 yrs, who will consume both a high AS (25% total energy) and low AS (5% total energy) diet for 7 days each, in a random order. Study 2 will include 325 children aged 6-18 yrs, who will complete five laboratory sessions. Record- assisted 24-hr dietary recalls will be completed at four of the sessions to assess habitual AS intake, and fingerstick blood samples will be obtained at two of the sessions. The potential confounding effects of non- sweetener corn and animal product consumption will be addressed in both studies by quantifying non- sweetener corn consumption in the controlled diets (Study 1) and in self-reported dietary recalls (Study 2), and by assessing the nitrogen stable isotope composition ¿15N of fingerstick samples. To advance existing knowledge of dietary assessment approaches, urinary sugars and urine d13C will be assessed in Study 1, which will permit a direct comparison of biomarkers - existing (urinary sucrose, fructose) and novel (urine and fingerstick d13C ). The role of AS in health has been contentious for decades, and the reliance on self-reported intake data is an often-cited flaw in this area. Our findings could significantly advance research addressing the health impacts of AS intake in children and adolescents.

描述（由适用提供）：已经建议消耗含能源的添加糖（AS），尤其是糖味的育种者（SSB），被认为是体重增加的贡献者。在儿童和青少年中，总摄入量约占总能量的16％，约为300-400 kcal/d； SSB完成了总摄入量的约50％。尽管受到主要卫生组织的认可，但AS及其主要食品来源的作用SSB在肥胖和相关合并症的发展和发展中仍然存在争议。这一领域的一个共同研究限制是提醒人们对饮食摄入量的自我报告的措施，这在研究儿童时会带来其他挑战。这就是认识到对评估饮食摄入量的客观方法的需求，例如AS消费的生物标志物。我们已经确定了Fingerstick Blood D13C作为成人生物标志物的有效性，并旨在将我们的创新生物标志物扩展到儿童饮食研究。我们建议使用两种方法确定指尖血D13C作为儿童生物标志物的有效性和可靠性。首先，受控的喂养成分（研究1）将提供具有实际摄入量生物标志物的必要数据，并确定其检测AS摄入水平的能力。其次，横截面成分（研究2）将将生物标志物与自我报告的摄入数据进行比较，该数据采用类似于国家营养监测方法的方法（即NHANES）。研究1将包括30岁的12-18岁青少年，他们将以随机的顺序消耗高达（25％的总能量）和低为7天的（总能量）饮食7天。研究2将包括325名6-18岁的儿童，他们将完成五次实验室会议。记录辅助的24小时饮食召回将在四次会议上完成，以评估习惯为摄入量，而在两次会议中将获得指尖的血液样本。两种研究都将通过量化受控饮食中的非甜味玉米消耗（研究1）和自我报告的饮食召回（研究2），并评估氮稳定的同位素稳定的同位素组成。为了促进现有的饮食评估方法知识，将在研究1中评估尿糖和尿液D13C，这将允许直接比较生物标志物 - 现有的（尿蔗糖，果糖）和新颖（尿液和指尖D13C）。数十年来，AS在健康中的作用一直在争议，对自我报告的摄入数据的缓解是该领域经常被引用的缺陷。我们的发现可以大大提高研究，以解决儿童和青少年摄入量的健康影响。