d13C Added Sugar Intake Biomarker: Determining Validity in Children

d13C 添加糖摄入生物标志物：确定儿童的有效性

• 批准号: 8768638
• 负责人: BRENDA M DAVY
• 金额: $ 20.49万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-14 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8768638
• 关键词: Address; Adolescent; Adult; Animals; Applications Grants; Area; Beverages; Biological Markers; Blood; Blood specimen; Calories; Canes; Carbon; Child; Child health care; Clinical; Comorbidity; Consumption; Data; Development; Diet; Dietary Assessment; Dietary intake; Food; Fructose; Funding; Future; Goals; Guidelines; Health; Health Policy; Hour; Human; Intake; Knowledge; Laboratories; Measures; Memory; Methodology; Methods; Molasses; National Health and Nutrition Examination Survey; Nitrogen; Nutritional; Obesity; Outcome; Participant; Pathway interactions; Patient Self-Report; Plants; Preparation; Process; Public Health; Public Policy; Recommendation; Reliance; Reporting; Research; Role; Sampling; Schools; Social Desirability; Source; Staging; Sucrose; Sweetening Agents; Taxation; Techniques; Urine; Validation; Validity and Reliability; Weight; Weight Gain; Work; Youth; aged; base; comparative; feeding; forgetting; health organization; innovation; literacy; minimally invasive; novel; policy implication; public health relevance; stable isotope; sugar; sweetened beverage; urinary

DESCRIPTION (provided by applicant): Consumption of energy-containing added sugars (AS) and in particular, sugar-sweetened beverages (SSB), have been suggested as contributors to weight gain. In children and adolescents, total AS intake represents ~16% of total energy, or ~300-400 kcal/d; SSB comprise ~50% of total AS intake. Although recognized by major health organizations, the role of AS and their primary food source, SSB, in the development and progression of obesity and related co-morbidities remains controversial. A common research limitation in this area is a reliance on self-reported measures of dietary intake, which present additional challenges when studying children. Thus, the need for objective methods to assess dietary intake, such as biomarkers of AS consumption, has been recognized. We have established the validity of the fingerstick blood d13C AS biomarker in adults, and aim to expand our innovative biomarker to studies of diet in children. We propose to establish the validity and reliability of the fingerstick blood d13C AS biomarker in children using two approaches. First, a controlled feeding component (Study 1) will provide data necessary for validation of the biomarker with actual AS intake, and determine its ability to detect levels of AS intake. Second, a cross-sectional component (Study 2) will compare the biomarker to self-reported intake data, collected in a method similar to national nutritional surveillance methodology (i.e., NHANES). Study 1 will include 30 adolescents aged 12-18 yrs, who will consume both a high AS (25% total energy) and low AS (5% total energy) diet for 7 days each, in a random order. Study 2 will include 325 children aged 6-18 yrs, who will complete five laboratory sessions. Record- assisted 24-hr dietary recalls will be completed at four of the sessions to assess habitual AS intake, and fingerstick blood samples will be obtained at two of the sessions. The potential confounding effects of non- sweetener corn and animal product consumption will be addressed in both studies by quantifying non- sweetener corn consumption in the controlled diets (Study 1) and in self-reported dietary recalls (Study 2), and by assessing the nitrogen stable isotope composition ¿15N of fingerstick samples. To advance existing knowledge of dietary assessment approaches, urinary sugars and urine d13C will be assessed in Study 1, which will permit a direct comparison of biomarkers - existing (urinary sucrose, fructose) and novel (urine and fingerstick d13C ). The role of AS in health has been contentious for decades, and the reliance on self-reported intake data is an often-cited flaw in this area. Our findings could significantly advance research addressing the health impacts of AS intake in children and adolescents.

描述（由申请人提供）：摄入含能量的添加糖 (AS)，特别是含糖饮料 (SSB)，已被认为是导致儿童和青少年体重增加的因素，AS 摄入总量约占 16%。总能量，即约 300-400 kcal/d；SSB 占 AS 摄入总量的约 50%，尽管主要卫生组织认识到 AS 及其主要食物来源 SSB 在人体中的作用。肥胖和相关并发症的发生和进展仍然存在争议，该领域的一个常见研究局限性是依赖自我报告的饮食摄入量，这在研究儿童时提出了额外的挑战，因此需要客观的方法来评估饮食。摄入量，例如 AS 消耗的生物标志物，我们已经确定了指尖血液 d13C AS 生物标志物在成人中的有效性，并旨在将我们的创新扩展到儿童饮食研究中，我们建议建立有效性和可靠性。指尖血使用两种方法在儿童中使用 d13C AS 生物标记物（研究 1）将提供验证生物标记物与实际 AS 摄入量所需的数据，并确定其检测 AS 摄入量水平的能力。组成部分（研究 2）将生物标志物与自我报告的摄入量数据进行比较，这些数据以类似于国家营养监测方法（即 NHANES）的方法收集，研究 1 将包括 30 名 12-18 岁的青少年。研究 2 将包括 325 名 6-18 岁的儿童，他们将按随机顺序分别摄入高 AS（25% 总能量）和低 AS（5% 总能量）饮食。五次实验室会议将在其中四次会议中完成记录辅助的 24 小时饮食回忆，以评估习惯性 AS 摄入量，并在其中两次会议中获取指尖采血样本，以了解非甜味剂玉米的潜在混杂影响。这两项研究都将通过量化受控饮食（研究 1）和自我报告的饮食召回（研究 2）中的非甜味剂玉米消耗量以及评估氮稳定同位素组成来解决动物消费产品的问题。为了推进饮食评估方法的现有知识，研究 1 将评估尿糖和尿液 d13C，这将允许直接比较生物标志物 - 现有的（尿蔗糖、果糖）和新的（尿液和指尖血 d13C）。几十年来，AS 在健康中的作用一直存在争议，而对自我报告摄入量数据的依赖是该领域经常被引用的缺陷。显着推进针对儿童和青少年摄入 AS 的健康影响的研究。