Detection of HPV infection in the oral cavity of Hispanics with and without periodontal disease

患有和不患有牙周病的西班牙裔人口腔中 HPV 感染的检测

• 批准号: 8823312
• 负责人: ANA Patricia ORTIZ
• 金额: $ 22.5万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-05 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8823312
• 关键词: Address; Adult; Area; Attention; Behavioral; Behavioral Mechanisms; Biochemical; Biological; Biopsy; Body mass index; Characteristics; Chronic; Cohort Studies; Computer Assisted; Cross-Sectional Studies; Data; Dental; Dental Epidemiology; Detection; Development; Dot Immunoblotting; Epidemiology; Ethnic group; Etiology; Frequencies; Funding; General Population; Genotype; Gingiva; HPV-High Risk; Head and Neck Cancer; Health; Hispanics; Human Papilloma Virus Vaccine; Human Papillomavirus; Human papilloma virus infection; Human papillomavirus 16; Individual; Infection; Inflammation; Interview; Knowledge; Lesion; Life; Life Style; Longitudinal Studies; Low income; Malignant Neoplasms; Measures; Medical; Methodology; Methods; Minority; National Health and Nutrition Examination Survey; National Institute of Dental and Craniofacial Research; Natural History; Oncogenic; Oral; Oral cavity; Oral health; Oral mucous membrane structure; Overweight; Parents; Patients; Periodontal Diseases; Periodontal Pocket; Periodontitis; Population; Population Group; Population Study; Poverty; Prevalence; Prevention strategy; Preventive; Public Health; Puerto Rican; Puerto Rico; Race; Recording of previous events; Research; Research Infrastructure; Risk; Risk Factors; Role; Sample Size; Severity of illness; Sexually Transmitted Diseases; Subgroup; Survey Methodology; Testing; Tissues; Tooth structure; Uncertain Risk; Underserved Population; United States; United States National Institutes of Health; Work; aged; base; cancer prevention; cohort; cost; design; edentulism; health disparity; high risk; interest; malignant mouth neoplasm; malignant oropharynx neoplasm; member; metropolitan; modifiable risk; non-oncogenic; oral infection; oral lesion; public health relevance; screening; social; socioeconomics; tool; uptake; viral detection

DESCRIPTION (provided by applicant): The rising rates of HPV-positive oropharyngeal cancers in the United States (US) and worldwide are a significant public health concern. In the US, it is estimated that 47% (~65 million) of adults aged e30 years have periodontal disease. This is of concern as studies suggest that chronic periodontitis is associated with systemic health conditions, including oral cancers. Research evaluating the role of local oral factors on natural history of oral HPV is lacking, critical information to develop effective prevention strategies. Given the scarcity of research evaluating the role of chronic oral inflammation on the natural history of oral HPV infection, assessment of the prevalence, genotypes, and correlates of oral HPV infection in the general population is a step toward a better understanding of the burden of this infection and its potential role with oral malignancies. The present cross-sectional study will capitalize on the already established infrastructure of the Study of Overweight Adults Longitudinal Study (SOALS), an ongoing NIH-funded cohort of 1,214 Hispanic adults aged 40-65 years living in the San Juan metropolitan area of Puerto Rico, to design and implement a cross-sectional study to start addressing the gaps described. Specifically, we propose to select a random subsample of 600 non-institutionalized adults who participate in SOALS to assess the prevalence and type distribution of oral HPV infection (overall, high-risk and concurrent multiple-type) in adults with and without periodontal disease, and to assess the association between HPV infection and periodontal disease severity. For the current study, assessment of both periodontal disease and oral HPV testing will be performed using validated NHANES methodology. Measures of risk factors for oral HPV infection will be collected using the audio computer-assisted self-interview (ACASI) methodology, a useful tool for collecting sensitive risk behavioral data. Data on dental assessment, selected sociodemographic characteristics, body mass index, lifestyles, medical and dental histories, and biochemical testing will be obtained from the parent study. For HPV typing, PCR products will be typed by dot-blot hybridization using 38 type-specific probes and two separate mixtures, including oncogenic and non-oncogenic HPV types. To our knowledge, this is the first study that will assess the frequency of oral HPV infection in Hispanic adults with and without periodontal disease. The selected study population will provide us with a unique high-risk group where both periodontal disease and oral HPV infection would be higher. Hence, we can study this association with high efficiency and at a reasonable cost. If longitudinal studies in cancer-free individuals confirm that periodontal disease increases the risk of oral HPV infection, preventive public health strategies could be developed, as periodontal disease screening could identify individuals at increased risk of oral HPV infection and oral cancer, particularly among minority, low-income, and underserved populations where oral health disparities are prevalent.

描述（由申请人提供）：美国和全球的HPV阳性口咽癌的率上升是一个重大的公共卫生问题。在美国，估计有47％（约6500万）年龄30岁的成年人患有牙周疾病。这是令人关注的，因为研究表明慢性牙周炎与包括口服癌症在内的全身健康状况有关。缺乏评估局部口服因素在口服HPV自然历史上的作用的研究，这是制定有效预防策略的关键信息。考虑到评估慢性口腔炎症对口服HPV感染的自然史的作用的研究稀缺，评估患病率，基因型和口腔HPV感染对普通人群中的相关性是对这种对这种感染的负担及其在口腔恶性中的潜在作用的一步。目前的横截面 研究将利用已经建立的超重成年人纵向研究（SOALS）研究的基础设施，这是NIH资助的1,214名西班牙裔成年人，居住在40-65岁的西班牙裔成年人，居住在波多黎各Ricco的San Juan Metropolitan地区，以设计和实施横断面的研究，以设计和实施跨越的glaps gaps。具体而言，我们建议选择一个参与Soals的600名非制度化成年人的随机子样本，以评估患有和没有牙周疾病的成年人的口服HPV感染（总体，高危和同时多种类型）的患病率和类型分布，并评估HPV感染与周期性疾病的关联和牙周疾病严重程度。对于当前的研究，将使用经过验证的NHANES方法对牙周疾病和口服HPV测试进行评估。口服HPV感染的危险因素的度量将使用音频计算机辅助自我浏览（ACASI）方法来收集，这是收集敏感风险行为数据的有用工具。有关牙齿评估，选定的社会人口统计学特征，体重指数，生活方式，医学和牙科历史以及生化测试的数据。对于HPV键入，使用38种类型的探针和两种单独的混合物（包括致癌性HPV类型），将通过点印刷杂交来键入PCR产品。据我们所知，这是第一项将评估有和没有牙周疾病的西班牙裔成年人口服HPV感染频率的研究。选定的研究人群将为我们提供一个独特的高风险群体，牙周疾病和口服HPV感染都会更高。因此，我们可以以高效率和合理的成本研究这种关联。如果无癌个体的纵向研究确认牙周疾病会增加口服HPV感染的风险，则可以制定预防性公共卫生策略，因为牙周疾病筛查可以鉴定出患有口腔HPV感染和口腔癌风险增加的个体，尤其是在少数群体，低收入人群以及口腔健康纠纷的人群中。