Quantitative Ultrasound Imaging for Noninvasive Assessment of Engineered Tissues

用于工程组织无创评估的定量超声成像

• 批准号: 8583800
• 负责人: CHERI X DENG
• 金额: $ 19.83万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8583800
• 关键词: Acoustics; Area; Biochemical; Biological Assay; Body Fluids; Bone Tissue; Cartilage; Cells; Clinic; Collagen; Complex; Data; Dependence; Development; Engineering; Frequencies; Goals; Hydrogels; Hydroxyapatites; Image; Imaging Techniques; In Vitro; Lead; Ligaments; Location; Measurement; Measures; Mechanics; Mediating; Methods; Minerals; Monitor; Morphology; Musculoskeletal; Orthopedics; Outcome; Particle Size; Phase; Process; Production; Property; Quality Control; Radiation; Research; Resolution; Sampling; Simulate; Spatial Distribution; Structure; Structure-Activity Relationship; System; Techniques; Technology; Tendon structure; Testing; Time; Tissue Engineering; Tissues; Transducers; Ultrasonography; commercialization; computerized data processing; density; elastography; experience; image processing; improved; insight; instrument; mineralization; particle; public health relevance; quantitative ultrasound; research and development; spatiotemporal; tool; ultrasound microscopy

DESCRIPTION (provided by applicant): Noninvasive imaging and characterization of engineered tissues would greatly enable the development, testing, and commercialization of new therapies. Ultrasound imaging and microscopy have been used to characterize some aspects of tissue structure, but conventional ultrasound techniques are hampered by instrument-dependence and are limited to qualitative assessment of morphology. The objective of this two-year exploratory project is to develop a quantitative imaging technique for the rapid and high resolution characterization of the composition, structure and mechanical properties of mineralized engineered tissues. It applies high frequency spectral ultrasound imaging (SUSI) to create high resolution images and power spectra of mineral-containing tissues. Parametric analysis of the power spectra provides information on the location, density, and particle size of the mineral component. Augmentation of SUSI with acoustic radiation force (ARF) elastography allows measurement of the local and bulk mechanical properties. The project has three Specific Aims (SA). In SA1 we will demonstrate the use of SUSI to quantitatively characterize the spatiotemporal development of a mineral phase in three dimensional (3D) engineered orthopaedic tissues. In SA2 we will implement ARF elastography for noncontact measurement of tissue mechanical properties in 3D engineered orthopaedic tissues. Finally, in SA3 we will correlate the composition and structure information from SA1 with the mechanical property data from SA2. Replacing the currently used destructive biochemical and histological methods of tissue characterization with noninvasive, spatiotemporal characterization of tissue development would greatly enhance research in tissue engineering. In addition, such methods would facilitate quality control and monitoring of engineered tissue products. The ability to correlate composition and mechanical properties in developing tissues over time also could lead to new insights into structure-function relationships and mechanobiology.

描述（由申请人提供）：工程组织的无创成像和表征将极大地促进新疗法的开发、测试和商业化。超声成像和显微镜已被用来表征组织结构的某些方面，但传统超声技术受到仪器依赖性的阻碍，并且仅限于形态的定性评估。这个为期两年的探索项目的目标是开发一种定量成像技术，用于快速、高分辨率地表征矿化工程组织的成分、结构和机械性能。它应用高频频谱超声成像 (SUSI) 来创建含矿物质组织的高分辨率图像和功率谱。功率谱的参数分析提供了有关矿物成分的位置、密度和颗粒尺寸的信息。通过声辐射力 (ARF) 弹性成像增强 SUSI，可以测量局部和整体机械性能。该项目有三个具体目标 (SA)。在 SA1 中，我们将演示如何使用 SUSI 来定量表征三维 (3D) 工程骨科组织中矿物相的时空发育。在 SA2 中，我们将实施 ARF 弹性成像技术，以非接触式测量 3D 工程骨科组织中的组织机械特性。最后，在 SA3 中，我们将 SA1 的成分和结构信息与 SA2 的机械性能数据相关联。用组织发育的非侵入性时空表征取代目前使用的组织表征的破坏性生化和组织学方法将极大地加强组织工程的研究。此外，此类方法将有助于工程组织产品的质量控制和监测。随着时间的推移，将发育中的组织的成分和机械特性关联起来的能力也可能带来对结构-功能关系和机械生物学的新见解。