Sleep and Associative Memory Formation in Aplysia

海兔的睡眠和联想记忆的形成

基本信息

批准号：
8771695
负责人：
Lisa Carlson Lyons
金额：
$ 22.8万
依托单位：
FLORIDA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-01 至 2016-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8771695
关键词：
Acute Adopted Affect Animal Model Animals Aplysia Arousal Aversive Stimulus Behavior Behavioral Biological Assay Cell physiology Chronic Circadian Rhythms Development Exhibits Feeding behaviors Food Foundations Functional disorder Future Gene Expression Gene Expression Profile Genes Goals Health Hour Human In Situ Hybridization Individual Industrial Accidents Injury Invertebrates Investigation Jet Lag Syndrome Learning Location Marines Memory Methods Modeling Molecular Neuronal Plasticity Neurons Occupational Occupational Accidents Pathway interactions Pattern Performance Plastics Posture Process Productivity Protein Isoforms Proteins RNA Sequences Recording of previous events Research Rest Short-Term Memory Sleep Sleep Deprivation Sleep Disorders Societies System Therapeutic Traffic accidents Training Ubiquitin Work base circadian pacemaker cognitive function cost improved in vivo insight long term memory meetings molecular dynamics multicatalytic endopeptidase complex neural circuit neuronal circuitry public health relevance research study response shift work social therapy development trafficking transcriptome sequencing

项目摘要

DESCRIPTION (provided by applicant): Memory formation represents a dynamic process modulated by multiple factors including the circadian clock and sleep. In the past few decades, sleep loss and sleep deprivation have developed into major societal problems with individuals working longer hours, fragmented sleep patterns due to shift work, technological interferences and social jet lag. Sleep loss and sleep deprivation exact a heavy toll on society and individuals through increased traffic accidents, increased industrial and occupational accidents, and decreased productivity and performance. Sleep deprivation causes significant decrements in short and long-term memory. In order to ameliorate the consequences of sleep deprivation, it is necessary to understand how systems level problems and changes in behavior affect molecular and cellular processes at the level of individual neuronal circuits and neuron groups. Defining the mechanisms through which sleep and sleep loss impact memory formation is crucial to identifying methods to optimize performance and health in modern society. Given the high molecular conservation underlying memory formation across species, the marine mollusk Aplysia californica with its simple neural circuitry provides an ideal model for detailing in vivo interactions between sleep, the circadian clock and memory. Previously, we described short, intermediate and long-term memory for an operant learning paradigm, learning that food is inedible (LFI). We have characterized sleep in Aplysia and found that Aplysia sleep patterns resemble human sleep patterns with sleep occurring only at night in long consolidated bouts. Our long-term objective is to define the mechanisms through which the sleep deprivation affects the formation of associative memories and identify mechanisms through which the circadian clock and sleep interact to regulate memory. The goal of this proposal is to identify cellular changes and changes in gene expression caused by sleep deprivation specifically in the neurons involved in feeding behaviors and LFI memory and to identify genes and pathways necessary for long-term memory formation that are inhibited by sleep deprivation. These studies will further our understanding of the functions of sleep and importantly, provide a research base for future therapeutic treatments to improve memory and performance.

描述（由申请人提供）：内存形成表示一个动态过程，该过程由多个因素调制，包括昼夜节律时钟和睡眠。在过去的几十年中，睡眠丧失和睡眠剥夺已发展为主要社会问题，个人工作时间更长，由于转移工作，技术干扰和社交喷气滞后而导致的睡眠模式分散。睡眠丧失和睡眠剥夺通过增加交通事故，工业和职业事故增加以及生产力和绩效降低，对社会和个人造成了沉重的损失。睡眠剥夺会导致短期和长期记忆的显着减少。为了减轻睡眠剥夺的后果，有必要了解系统水平问题以及行为的变化如何影响单个神经元电路和神经元组水平的分子和细胞过程。定义睡眠和睡眠损失影响记忆形成的机制对于确定优化现代社会绩效和健康的方法至关重要。鉴于物种之间的高分子保护基础记忆形成，及其简单神经回路的海洋软体动物Aplysia加利福尼亚为详细介绍了睡眠，昼夜节律时钟和记忆之间体内相互作用的理想模型。以前，我们描述了操作人员学习范式的短期，中间和长期记忆，了解食物是不可食用的（LFI）。我们已经表征了Aplysia的睡眠，发现Aplysia的睡眠模式类似于人类的睡眠模式，而睡眠仅在晚上才在长时间的固结爆发中发生。我们的长期目标是定义睡眠剥夺影响相关记忆形成的机制，并确定昼夜节律时钟和睡眠相互作用以调节记忆的机制。该提案的目的是确定由睡眠剥夺引起的细胞变化和基因表达的变化，这些基因表达是在喂养行为和LFI记忆中涉及的神经元中特异性的，并确定由睡眠剥夺抑制的长期记忆形成所必需的基因和途径。这些研究将进一步理解睡眠功能，并重要的是，为未来的治疗治疗提供了研究基础，以改善记忆力和性能。