BIOMARKERS CORE

生物标志物核心

• 批准号: 8153475
• 负责人: STEPHEN S HECHT
• 金额: $ 18.75万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8153475
• 关键词: 1,3-Butadiene; 1-Butanol; 2-butenal; Abbreviations; Acetylcysteine; Acids; Acrolein; Alkaloids; Anabasine; Arachidonic Acids; Benzene; Biological Markers; Butanones; Carcinogens; Chemicals; Cigarette; Cotinine; DNA; DNA Adducts; Dinoprostone; Ethylene Oxide; Evaluation; Formaldehyde; Glucuronides; Human; Inflammation; Instruction; Laboratories; Leukocytes; Malignant Neoplasms; Methods; Minor; N' -nitrosonornicotine; Nicotine; Parents; Principal Investigator; Procedures; Program Research Project Grants; Public Health; Quality Control; Research; Research Personnel; Resources; Series; Services; Sum; Tobacco; Tobacco use; Tobacco-Associated Carcinogen; Urine; hydroxycotinine; nornicotine; oxidative damage; phenanthrene; prostaglandin M; tobacco carcinogenesis; tool; toxicant; tumor; urinary

The Tobacco Constituent and Biomarker Assessment Core will provide a unique resource for Projects 1-3. Investigators in this Core comprise one of the leading academic laboratories in the world for chemical analysis of tobacco constituents and related metabolites and biomarkers. The following urinary metabolites will be quantified: nicotine equivalents (the total of nicotine, cotinine, and trans-3'-hydroxycotinine and their glucuronides), total NNAL (the total of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its glucuronides), total NNN (the total of free and glucuronidated A/'-nitrosonornicotine), mercapturic acids of the volatile carcinogens 1,3-butadiene, benzene, acrolein, crotonaldehyde, and ethylene oxide, and the inflammation and oxidative damage biomarkers 11-a-hydroxy-9,15-dioxo-2,3,4,5-tetranorprostane-1,20-dioic acid (PGE- M) and 9,11,15-trihydroxyprosta-5,13-dien-1-oic acid. We will also quantify the formaldehyde-DNA adduct, /V^-hydroxymethyideoxyadenosine in leukocyte DNA, and minor tobacco alkaloids in urine. These biomarkers will provide crucial information for the research being carried out in Projects 1-3. RELEVANCE (See instructions): Methods for achieving reduction in public health harm from use of tobacco products are critical for decreasing the human cancer toll of tobacco use. The biomarkers being quantified in this core are representative of many of the major carcinogenic and tumor enhancing constituents known to be present in tobacco products. Quantifying these biomarkers will provide an important tool for the evaluation of tobacco products to determine their potential impact on public health.

烟草成分和生物标志物评估核心将为项目 1-3 提供独特的资源。 该核心的研究人员包括世界领先的化学学术实验室之一 烟草成分及相关代谢物和生物标志物的分析。以下尿代谢物 将被量化：尼古丁当量（尼古丁、可替宁和反式 3'-羟基可替宁的总量及其 葡萄糖醛酸苷)、总NNAL(4-(甲基亚硝基氨基)-1-(3-吡啶基)-1-丁醇及其葡萄糖醛酸苷的总量)、 总NNN（游离和葡萄糖醛酸化A/'-亚硝基降烟碱的总量）、挥发性硫醇尿酸 致癌物质1,3-丁二烯、苯、丙烯醛、巴豆醛和环氧乙烷，以及炎症 和氧化损伤生物标志物 11-a-羟基-9,15-二氧代-2,3,4,5-四降前列腺素-1,20-二酸 (PGE- M)和9,11,15-三羟基前列腺-5,13-二烯-1-油酸。我们还将量化甲醛-DNA 加合物， 白细胞DNA中的N 1 -羟甲基脱氧腺苷和尿液中的少量烟草生物碱。这些 生物标志物将为项目 1-3 中正在进行的研究提供重要信息。 相关性（参见说明）： 减少使用烟草制品造成的公共健康危害的方法对于 减少烟草使用导致的人类癌症死亡人数。该核心中量化的生物标志物是 代表许多已知存在于的主要致癌和肿瘤增强成分 烟草制品。量化这些生物标志物将为评估烟草提供重要工具 产品以确定其对公共健康的潜在影响。