A High Throughput Assay for Neural Crest Cell Migration & Toxicity

神经嵴细胞迁移的高通量测定

基本信息

批准号：
8929396
负责人：
PATRICK M MCDONOUGH
金额：
$ 107.48万
依托单位：
VALA SCIENCES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-31 至 2016-07-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8929396
关键词：
Achievement Area Biological Assay Cell Count Cells Contracts Data Discrimination Embryo Embryonic Development Goals Health Histocompatibility Testing Image Analysis Methods Microscopy Migration Assay Mitochondria Neural Crest Neural Crest Cell Neurons Peripheral Phase Process Research Sampling Site Small Business Innovation Research Grant Smooth Muscle Staging Testing Toxic effect base bone cell motility cellular imaging digital high throughput screening human embryonic stem cell improved novel

Achievement Area Biological Assay Cell Count Cells Contracts Data Discrimination Embryo Embryonic Development Goals Health Histocompatibility Testing Image Analysis Methods Microscopy Migration Assay Mitochondria Neural Crest Neural Crest Cell Neurons Peripheral Phase Process Research Sampling Site Small Business Innovation Research Grant Smooth Muscle Staging Testing Toxic effect base bone cell motility cellular imaging digital high throughput screening human embryonic stem cell improved novel

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项目摘要

This Fast-Track SBIR contract will develop a high throughput assay to test compounds for toxic effects on Neural Crest cells. The Neural Crest forms, transiently, within the embryo; the Neural Crest cells then migrate to different sites and develop into various tissue types, including peripheral neurons, glial and smooth muscle, and bone. Thus, compounds that inhibit Neural Crest cells migration or have other toxic effects, will have profound effects on embryonic development. We have developed a Neural Crest Cell Migration assay Neural Crest cells, utilizing cells derived from human embryonic stem cells. The cells are seeded in multi-well plates, allowed to reach confluence, then "scratched" to form a cell-free region; test compounds are then added and the number of cells that migrate into the area are quantified via automated digital microscopy.

该快速轨道SBIR合同将开发高通量测定法，以测试化合物对神经rest细胞的毒性作用。神经波峰在胚胎中瞬时形成；然后，神经rest细胞迁移到不同的部位，并发展为各种组织类型，包括周围神经元，神经胶质和平滑肌以及骨骼。因此，抑制神经rest细胞迁移或具有其他毒性作用的化合物将对胚胎发育产生深远的影响。我们使用了源自人类胚胎干细胞的细胞，开发了一种神经rest细胞迁移测定神经rest细胞。将细胞接种在多孔板中，允许到达汇合，然后“刮擦”以形成一个无细胞的区域。然后添加测试化合物，并迁移到该区域的单元格数为通过自动数字显微镜进行量化。