A High Throughput Assay for Neural Crest Cell Migration & Toxicity

神经嵴细胞迁移的高通量测定

• 批准号: 8929396
• 负责人: PATRICK M MCDONOUGH
• 金额: $ 107.48万
• 依托单位: VALA SCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-31 至 2016-07-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8929396
• 关键词: Achievement; Area; Biological Assay; Cell Count; Cells; Contracts; Data; Discrimination; Embryo; Embryonic Development; Goals; Health; Histocompatibility Testing; Image Analysis; Methods; Microscopy; Migration Assay; Mitochondria; Neural Crest; Neural Crest Cell; Neurons; Peripheral; Phase; Process; Research; Sampling; Site; Small Business Innovation Research Grant; Smooth Muscle; Staging; Testing; Toxic effect; base; bone; cell motility; cellular imaging; digital; high throughput screening; human embryonic stem cell; improved; novel

This Fast-Track SBIR contract will develop a high throughput assay to test compounds for toxic effects on Neural Crest cells. The Neural Crest forms, transiently, within the embryo; the Neural Crest cells then migrate to different sites and develop into various tissue types, including peripheral neurons, glial and smooth muscle, and bone. Thus, compounds that inhibit Neural Crest cells migration or have other toxic effects, will have profound effects on embryonic development. We have developed a Neural Crest Cell Migration assay Neural Crest cells, utilizing cells derived from human embryonic stem cells. The cells are seeded in multi-well plates, allowed to reach confluence, then "scratched" to form a cell-free region; test compounds are then added and the number of cells that migrate into the area are quantified via automated digital microscopy.

该快速通道 SBIR 合同将开发一种高通量测定法，以测试化合物对神经嵴细胞的毒性作用。神经嵴在胚胎内短暂形成；然后，神经嵴细胞迁移到不同部位并发育成各种组织类型，包括外周神经元、神经胶质和平滑肌以及骨骼。因此，抑制神经嵴细胞迁移或具有其他毒性作用的化合物将对胚胎发育产生深远的影响。我们利用人类胚胎干细胞衍生的细胞，开发了一种神经嵴细胞迁移测定法。将细胞接种到多孔板中，使其达到汇合，然后“划伤”以形成无细胞区域；然后添加测试化合物，迁移到该区域的细胞数量为 通过自动数字显微镜进行量化。