Special Mouse Strains Resource

特殊小鼠品系资源

• 批准号: 8663972
• 负责人: Cathleen M Lutz
• 金额: $ 81.86万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8663972
• 关键词: Animals; Award; Biomedical Research; Breeding; Candidate Disease Gene; Caring; Cell Culture Techniques; Cells; Collection; Communities; Complex; Consomic Strain; Data; Derivation procedure; Disease; Disease model; ES Cell Line; Environmental Risk Factor; Funding; Gene Mutation; Gene Targeting; Genes; Genetic; Genotype; Germ Lines; Goals; Grant; Health Status; Hereditary Disease; Human; Human Genome; In Vitro; Inbreeding; Individual; Life; Location; Maps; Mouse Strains; Mus; National Center for Research Resources; Oral; Phenotype; Recombinants; Research Personnel; Resolution; Resources; Scientist; Severities; Testing; Writing; base; consomic; developmental genetics; embryonic stem cell; genetic analysis; genetic resource; human disease; improved; in vitro Model; mouse genome; mouse model; parent grant; permissiveness; pluripotency; research study; segregation; tool; trait

DESCRIPTION (provided by applicant): Many common and pervasive human diseases are genetically complex; that is, they result from the interaction of multiple genetic, developmental, and environmental factors. Even single gene mutation diseases are frequently modulated in onset, severity, and progression by modifier genes. The long-term objective of this project, the Special Mouse Strains Resource (SMSR), is to maintain, distribute, and provide information for genetic tools for analyzing complex traits. The resources in the SMSR currently include six Recombinant Inbred (Rl) and two Chromosomal Substitution (CS) strain sets of mice that are valuable tools to improve the efficiency of identifying genes in mouse models of human multigenic diseases. These sets of mouse strains provide a perpetual resource for phenotyping with cumulative genotyping. A major new initiative in this application is a proposal to enhance the power of the C57BL/6J- Chr#A/NaJ Chromosomal Substitution (consomic) set by developing a set of germ line competent, embryonic stem cell lines from the individual strains to be used for testing candidate genes in co-isogenic strain backgrounds, cellular phenotyping, and in-vitro modeling of quantitative traits. The Specific Aims of the grant are to: 1) maintain strains of mice currently included in the SMSR as live colonies and to distribute these mice to investigators; 2) enhance the value of the SMSR strains by making, and distributing, ES cell lines for each of the C57BL/6J-Chr#A/NaJ consomic strains; and 3) provide complete and accurate information for SMSR strains that is easily accessible to the scientific community. Identification of genes responsible for complex diseases in mouse models often predicts the location of human disease genes, based on the conserved regions of mouse and human genomes; consequently, these resources significantly contribute to the resolution of complex genetic diseases in humans.

描述（由申请人提供）：许多常见和普遍的人类疾病在遗传上很复杂；也就是说，它们是由于多种遗传，发育和环境因素的相互作用而产生的。即使是单个基因突变疾病，也经常通过修饰符基因的发作，严重程度和进展调节。该项目的长期目标，特殊的小鼠菌株资源（SMSR）是要维护，分发和提供用于分析复杂性状的遗传工具的信息。 SMSR中的资源目前包括六种重组近交（RL）和两个小鼠的染色体替代（CS）菌株集，它们是提高人类多基因疾病小鼠模型中鉴定基因的效率的宝贵工具。这些小鼠菌株提供了一种永久性的资源，用于累积基因分型。该应用程序中的一项主要新计划是一项提案，以增强C57BL/6J-CHR＃A/NAJ染色体替代（复杂）（复杂）的功能，该建议是通过开发一组竞争性的种系竞争力系，来自各个菌株的胚胎干细胞系，用于在共同基因造成的背景，细胞型，量子photot量和模型中，用于测试候选基因的候选基因。赠款的具体目的是：1）维持SMSR中目前包括作为活菌落中的小鼠菌株，并将这些小鼠分配给研究人员； 2）通过制造和分布C57BL/6J-CHR＃A/NAJ辅助菌株的ES细胞系来增强SMSR菌株的值； 3）为SMSR菌株提供完整而准确的信息，科学界很容易获得。小鼠模型中负责复杂疾病的基因的鉴定通常会根据小鼠和人类基因组的保守区域来预测人类疾病基因的位置。因此，这些资源显着有助于解决人类复杂的遗传疾病。