An instrument for direct exposure of cell cultures to airborne particulate matter

一种将细胞培养物直接暴露于空气颗粒物的仪器

• 批准号: 8453292
• 负责人: Arantzazu Eiguren Fernandez
• 金额: $ 14.98万
• 依托单位: AEROSOL DYNAMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-12-07 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8453292
• 关键词: Acute Disease; Address; Adverse effects; Air Pollution; Airborne Particulate Matter; Biological; Caliber; Carbon Dioxide; Cell Culture Techniques; Cell Survival; Cells; Chemicals; Chronic Disease; Collection; Complement; Deposition; Development; Devices; Encapsulated; Environmental Exposure; Epidemiologic Studies; Exposure to; Goals; Growth; Health; In Vitro; Incubated; Incubators; Indoor Air Quality; Laboratories; Lead; Link; Liquid substance; Measurement; Measures; Metabolic; Methods; Modeling; Monitor; Morphologic artifacts; National Institute of Environmental Health Sciences; Occupational; Occupations; Outcome; Oxidative Stress; Oxidative Stress Induction; Particulate Matter; Phase; Physical condensation; Physiological; Polystyrenes; Property; Public Health; Research Personnel; Risk Assessment; Sampling; Solutions; Survival Rate; System; Technology; Time; Toxic effect; Water; Work; adverse outcome; air filter; base; cell growth; exposed human population; field study; high risk; improved; innovation; instrument; nanoparticle; novel strategies; particle; particle counter; particle exposure; phase 1 study; pollutant; portability; public health relevance; response; tool; ultrafine particle

DESCRIPTION (provided by applicant): Cell culture studies allow air pollution researchers to assess toxicological outcomes that can be later used in human exposure models and risk assessment studies. However, there are only a few collection systems that allow direct cellular exposure to ambient pollutants. All these systems, due to their large size and complexity, are laboratory devices and cannot be deployed easily for field studies. In this project we propose a new approach for a more direct and faster assessment of in-vitro toxicity of airborne particulate matter (PM). The main objective is to develop a new sampling system that allows direct, real-time exposure of cells to PM, which reduces the extent of sampling artifacts as well as sample manipulation prior to cell exposure. The advantage of this new system compared with the existing ones is that it uses water-based particle growth for efficient collection and deposition o particles with aerodynamic diameters ranging from 5 nm to 5 ?m. This technology also avoids the most common problem associated with other particle collection systems, the possibility of changing the physical, chemical and toxicological properties of the particles prior to cell exposure. In addition, its small size and low power requirements make the device suitable for both laboratory work and field deployment. The approach offers a new alternative for direct cellular exposure studies. It provides more accurate results when studying cellular responses to PM exposure and thus a better understanding of the possible adverse outcomes related with exposure to ambient pollutants. Our goal will be achieved by completing the following specific aims: i) adapt the existing collection chamber to maintain environmental conditions suitable for viable and healthy cells; and ii) conduct laboratory controlled exposure studies to evaluate whether our approach leads to identifiable biological responses and cell toxicity. In summary, our device brings together a well established technology with innovative applications, providing the means to expose cells directly to airborne particles, and to measure more accurately metabolic responses associated with exposure to airborne PM.

描述（由申请人提供）：细胞培养研究使空气污染研究人员能够评估毒理学结果，随后可用于人体暴露模型和风险评估研究。然而，只有少数收集系统允许细胞直接暴露于环境污染物。所有这些系统由于尺寸大且复杂，都是实验室设备，无法轻松部署用于现场研究。在这个项目中，我们提出了一种新方法，可以更直接、更快速地评估空气颗粒物 (PM) 的体外毒性。主要目标是开发一种新的采样系统，允许细胞直接、实时暴露于 PM，从而减少采样伪影的程度以及细胞暴露之前的样品操作。与现有系统相比，该新系统的优点在于，它利用水基颗粒生长来有效收集和沉积空气动力学直径范围为 5 nm 至 5 µm 的颗粒。该技术还避免了与其他颗粒收集系统相关的最常见问题，即在细胞暴露之前改变颗粒的物理、化学和毒理学特性的可能性。此外，其小尺寸和低功耗要求使得该设备既适合实验室工作也适合现场部署。该方法为直接细胞暴露研究提供了一种新的替代方案。在研究细胞对 PM 暴露的反应时，它可以提供更准确的结果，从而更好地了解与暴露于环境污染物相关的可能的不利结果。我们的目标将通过完成以下具体目标来实现：i）调整现有的收集室以维持适合存活和健康细胞的环境条件； ii) 进行实验室控制暴露研究，以评估我们的方法是否会导致可识别的生物反应和细胞毒性。总之，我们的设备将成熟的技术与创新应用结合在一起，提供了将细胞直接暴露于空气颗粒的方法，并更准确地测量与空气颗粒物暴露相关的代谢反应。