An instrument for direct exposure of cell cultures to airborne particulate matter

一种将细胞培养物直接暴露于空气颗粒物的仪器

• 批准号: 8453292
• 负责人: Arantzazu Eiguren Fernandez
• 金额: $ 14.98万
• 依托单位: AEROSOL DYNAMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-12-07 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8453292
• 关键词: Acute Disease; Address; Adverse effects; Air Pollution; Airborne Particulate Matter; Biological; Caliber; Carbon Dioxide; Cell Culture Techniques; Cell Survival; Cells; Chemicals; Chronic Disease; Collection; Complement; Deposition; Development; Devices; Encapsulated; Environmental Exposure; Epidemiologic Studies; Exposure to; Goals; Growth; Health; In Vitro; Incubated; Incubators; Indoor Air Quality; Laboratories; Lead; Link; Liquid substance; Measurement; Measures; Metabolic; Methods; Modeling; Monitor; Morphologic artifacts; National Institute of Environmental Health Sciences; Occupational; Occupations; Outcome; Oxidative Stress; Oxidative Stress Induction; Particulate Matter; Phase; Physical condensation; Physiological; Polystyrenes; Property; Public Health; Research Personnel; Risk Assessment; Sampling; Solutions; Survival Rate; System; Technology; Time; Toxic effect; Water; Work; adverse outcome; air filter; base; cell growth; exposed human population; field study; high risk; improved; innovation; instrument; nanoparticle; novel strategies; particle; particle counter; particle exposure; phase 1 study; pollutant; portability; public health relevance; response; tool; ultrafine particle

DESCRIPTION (provided by applicant): Cell culture studies allow air pollution researchers to assess toxicological outcomes that can be later used in human exposure models and risk assessment studies. However, there are only a few collection systems that allow direct cellular exposure to ambient pollutants. All these systems, due to their large size and complexity, are laboratory devices and cannot be deployed easily for field studies. In this project we propose a new approach for a more direct and faster assessment of in-vitro toxicity of airborne particulate matter (PM). The main objective is to develop a new sampling system that allows direct, real-time exposure of cells to PM, which reduces the extent of sampling artifacts as well as sample manipulation prior to cell exposure. The advantage of this new system compared with the existing ones is that it uses water-based particle growth for efficient collection and deposition o particles with aerodynamic diameters ranging from 5 nm to 5 ?m. This technology also avoids the most common problem associated with other particle collection systems, the possibility of changing the physical, chemical and toxicological properties of the particles prior to cell exposure. In addition, its small size and low power requirements make the device suitable for both laboratory work and field deployment. The approach offers a new alternative for direct cellular exposure studies. It provides more accurate results when studying cellular responses to PM exposure and thus a better understanding of the possible adverse outcomes related with exposure to ambient pollutants. Our goal will be achieved by completing the following specific aims: i) adapt the existing collection chamber to maintain environmental conditions suitable for viable and healthy cells; and ii) conduct laboratory controlled exposure studies to evaluate whether our approach leads to identifiable biological responses and cell toxicity. In summary, our device brings together a well established technology with innovative applications, providing the means to expose cells directly to airborne particles, and to measure more accurately metabolic responses associated with exposure to airborne PM.

描述（由申请人提供）：细胞培养研究允许空气污染研究人员评估可以在人类暴露模型和风险评估研究中使用的毒理学结果。但是，只有少数收集系统可以直接暴露于环境污染物。所有这些系统，由于它们的尺寸较大和复杂性，都是实验室设备，无法轻松部署用于现场研究。在这个项目中，我们提出了一种新方法，以更直接，更快地评估空气中颗粒物（PM）的体外毒性。主要目的是开发一个新的采样系统，该系统允许细胞直接实时暴露于PM，从而降低了在细胞暴露之前的采样伪像以及样品操作的程度。与现有系统相比，这种新系统的优点是，它使用水基颗粒生长来有效收集和沉积O颗粒，其空气动力学直径范围为5 nm至5？m。该技术还避免了与其他粒子收集系统相关的最常见问题，即在细胞暴露之前改变颗粒的物理，化学和毒理学特性的可能性。此外，其尺寸小和低功率要求使该设备适合实验室工作和现场部署。该方法为直接细胞暴露研究提供了一种新的替代方法。在研究对PM暴露的细胞反应时，它提供了更准确的结果，从而更好地理解与暴露于环境污染物有关的可能的不良结果。我们的目标将通过完成以下特定目的来实现：i）适应现有的收集室以维持适合可行和健康细胞的环境条件； ii）进行实验室控制的暴露研究，以评估我们的方法是否导致可识别的生物学反应和细胞毒性。总而言之，我们的设备汇集了一项具有创新应用的良好技术，提供了将细胞直接暴露于空气颗粒的方法，并测量与暴露于机载PM相关的更准确的代谢反应。