CELL & TISSUE IMAGING FACILITY

细胞

• 批准号: 8519332
• 负责人: SIMON C WATKINS
• 金额: $ 14.7万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8519332
• 关键词: Achievement; Algorithms; Animals; Antigens; Award; Biological Assay; Brain; Brightfield Microscopy; Cancer Center; Cancer Center Support Grant; Cell Communication; Cells; Collection; Color; Communities; Complement; Computer Assisted; DNA; Data; Data Set; Dimensions; Electron Microscope; Electron Microscopy; Electrons; Ensure; Equipment; Event; Experimental Designs; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Fluorochrome; Four-dimensional; Freeze Fracturing; Frozen Sections; Funding; Gene Delivery; Gene Expression; Grant; Human Resources; Image; Image Analysis; Imagery; Imaging Device; Immunoelectron Microscopy; In Situ; Investigation; Investments; Label; Laser Scanning Microscopy; Life; Location; Measurement; Methodology; Methods; Microinjections; Micrometastasis; Microscope; Microscopic; Microscopic Autoradiography; Microscopy; Molecular; Optics; Organ; Organelles; Penetration; Phase-Contrast Microscopy; Photons; Proteins; Publishing; Research; Research Personnel; Resolution; Resources; Scanning; Scanning Transmission Electron Microscopy Procedures; Secure; Services; Signal Transduction; Spatial Distribution; Speed; Supporting Cell; System; Techniques; Technology; Time; Tissues; Tumor Tissue; Universities; University of Pittsburgh Cancer Institute; Work; cell killing; cell type; cellular imaging; charge coupled device camera; design; digital; digital imaging; fluorescence imaging; formycin triphosphate; image processing; imaging modality; improved; instrument; instrumentation; light microscopy; member; molecular marker; morphometry; neoplastic cell; novel; optical imaging; programs; ratiometric; submicron; technology development; tool; transmission process; tumor; two-photon; web site

Over the last several years, microscopy as a scientific tool has reinvented itself It has changed from a group of principally descriptive methodologies, to a wide range of primary tools and techniques to investigate the molecular organization of organs, tissues and cells. Advances in microscope and camera design, fluorescent dye technology and the development of fluorescent proteins and multiple other novel imaging methods including 2 photon. Fluorescence Resonance Energy Transfer Microscopy (FRET), and Total Internal Reflection Fluorescence Microscopy (TIRF) have made the simultaneous resolution and quantitation of multiple concurrent molecular markers for both protein and DNA at a sub-micron resolution a reality. The recognition of the potential utility of these techniques to improve the scientific investigations of the rapidly expanding research community at the University of Pittsburgh Cancer Institute (UPCI) led to the formation of a centralized microscope imaging center; the Center for Biologic Imaging (CBI) at the University of Pittsburgh in 1991. The UPCI Cell and Tissue Imaging Facility (CTIF) was started as an offshoot of CBI eleven years ago. Since then the CTIF has become an essential resource for most ofthe research programs within UPCI. The CTIF described in this narrative performs microscopic analyses services for members of the UPCI research community, ranging from the traditional to the cutting edge, using a full complement of microscope technologies. At the time of the last review of this facility in 2003, UPCI had just relocated to the Shadyside campus. In order to ensure that outstanding microscopy services would be available for researchers in Shadyside, with support from UPCI, a satellite facility ofthe CTIF has been established in the Hillman Cancer Center specifically \o supply imaging capabilities to researchers at this location. The ability to establish and support the CTIF satellite facility has required a significant investment in instrumentation and personnel. We will use the CTIF support requested in this application to provide support for personnel who provide advanced microscopy support at the main CTIF center on the Oakland campus and also to provide resources for the new satellite facility in the Shadyside campus.

在过去的几年中，显微镜作为一种科学工具已经重新发明了，它已经从 主要具有描述性的方法论，到了广泛的主要工具和技术 研究器官，组织和细胞的分子组织。显微镜和相机的进步 设计，荧光染料技术以及荧光蛋白和其他多种新型的开发 成像方法，包括2个光子。荧光共振能量转移显微镜（FRET）和 总内部反射荧光显微镜（TIRF）使得同时解决和 在亚微米分辨率A中对蛋白质和DNA的多个并发分子标记的定量 现实。 认识到这些技术的潜在效用，以改善对科学研究的研究 匹兹堡大学癌症研究所（UPCI）的迅速扩大的研究社区导致了 中央显微镜成像中心的形成；生物成像中心（CBI） 匹兹堡大学于1991年。UPCI细胞和组织成像设施（CTIF）开始为 十一年前的CBI分支。从那时起，CTIF已成为大多数人的重要资源 UPCI中的研究计划。此叙述中描述的CTIF执行了微观分析 UPCI研究社区成员的服务，从传统到最前沿， 使用显微镜技术的完整补充。在2003年对该设施进行了最后一次审查时， UPCI刚刚搬到了Shadyside校园。为了确保出色的显微镜服务 在upci的支持下，Shadyside的研究人员将为CTIF的卫星设施提供 是在希尔曼癌症中心建立的，专门为研究人员提供了成像功能 这个位置。建立和支持CTIF卫星设施的能力需要很大 对仪器和人员进行投资。我们将使用本应用程序中要求的CTIF支持 为在主要CTIF中心提供高级显微镜支持的人员提供支持 奥克兰校园，还为Shadyside校园的新卫星设施提供资源。