PROTEOMICS RESOURCE

蛋白质组学资源

• 批准号: 8637175
• 负责人: LAWRENCE S SHAPIRO
• 金额: $ 13.64万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-04 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8637175
• 关键词: Arts; Biological; Budgets; Cancer Center; Cancer Center Support Grant; Coupled; Data; Digestion; Education; Experimental Designs; Financial Support; Fund Raising; Funding; Future; Gel; Goals; Grant; Investigation; Investments; Label; Liquid substance; Malignant Neoplasms; Mass Spectrum Analysis; Methods; Peer Review; Phosphorylation Site; Post-Translational Protein Processing; Preparation; Protein Chemistry; Proteins; Proteomics; Proteomics Shared Resource; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resolution; Resource Sharing; Resources; Sampling; Services; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Techniques; Tissues; Two-Dimensional Gel Electrophoresis; anticancer research; base; cost effective; instrumentation; mass spectrometer; member; novel diagnostics; operation; protein complex; service utilization; structural biology; therapeutic target; tool

The Proteomics Shared Resource is a new Cancer Center SR that evolved from an established facility at CUMC with strong expertise in all aspects of sample preparation and mass spectrometric analysis that will be increasingly applied to cancer related research projects. The goal is to provide an essential battery of mass spectrometry-based proteomics tools to HICCC investigators. Specific services are to use state-ofthe- art experimental strategies for: ¿ Identification of proteins by in-gel digestion and mass spectrometry ¿ Identification of protein components within protein complexes ¿ Identification of phosphorylation sites and other covalent protein modifications Instrumentation in this facility includes two high-resolution quadrupole-TOF electrospray mass spectrometers equipped with nanoflow LC for LC-MS, and a MALDI-TOF mass spectrometer. A key activity will be education of researchers in the ways state-of-the-art proteomics tools can advance their research. The HICCC has made recent investments in the facility infrastructure, and has been publicizing the facility to HICCC members, leading to an increased focus on mass spectrometry for cancer research. However, currently the facility has minimal financial support for daily operating expenses from the HICCC and has raised funds for its continued operation by offering cost-effective mass spectrometry services to outside investigators. In this grant renewal we are proposing increased direct support for this Shared Resource. The facility will continue to have close intervhactions with investigators, advising them on experimental design, sample preparation, and data interpretation. Future goals include the implementation of quantitative proteomics techniques based on labeling methods coupled with LC-MS, and the expansion of the Resource to include a two-dimensional gel electrophoresis service. Gel-based proteomics will facilitate investigation of protein mixtures of greater complexity such as those found in tissues and biological fluids, and opens the possibility of finding novel diagnostic markers and therapeutic targets. During the last period of the CCSG (transitioning to Cancer Center management) 34% of the Columbia investigators using the facility were Center members with peer-reviewed funding, with those members representing over 30% of the utilization of the services. The proposed total operating budget of the facility is $380,006, of which we are requesting $84,858 from CCSG.

蛋白质组学共享资源是一个新的癌症中心SR，它从已建立的设施发展 CUMC在样本制备和质谱分析的各个方面具有强大的专业知识，这将 越来越多地应用于癌症相关的研究项目。目标是提供必不可少的电池 基于质谱的蛋白质组学工具用于HICCC研究者。特定的服务是使用最先进的 艺术实验策略： »通过凝胶消化和质谱法鉴定蛋白质 »鉴定蛋白质复合物中蛋白质成分 »鉴定磷酸化位点和其他共价蛋白质修饰 该设施中的仪器包括两个高分辨率四极电喷雾质量 配备LC-MS的纳米流LC和MALDI-TOF质谱仪的光谱仪。关键活动 将以最先进的蛋白质组学工具可以推进其研究的方式对研究人员进行教育。 HICCC最近对设施基础设施进行了投资，并一直在发布该设施 对于HICCC成员，导致对癌症研究的质谱法的关注越来越大。然而， 目前，该设施对HICCC的日常运营费用的财政支持最少，并且 通过向外部提供具有成本效益的质谱服务来为其继续运营筹集资金 调查人员。在此赠款续订中，我们提议对此共享资源的直接支持增加。 该设施将继续与调查人员有密切的间隔，并向他们咨询实验 设计，样本准备和数据解释。未来的目标包括实施 基于标签方法与LC-MS的定量蛋白质组学技术，并扩展 包括二维凝胶电泳服务的资源。基于凝胶的蛋白质组学将有助于 研究更复杂的蛋白质混合物，例如在组织和生物学流体中发现的蛋白质混合物， 并打开了寻找新颖的诊断标记和治疗靶标的可能性。 在CCSG的最后一个时期（过渡到癌症中心管理）34％的哥伦比亚 使用该设施的调查人员是由同行评审资金的中心成员，这些成员 占服务利用的30％以上。该设施的拟议总运营预算 是380,006美元，我们要求CCSG的$ 84,858。