Quorum Sensing Regulation of EHEC Virulence Genes

肠出血性大肠杆菌毒力基因的群体感应调控

基本信息

批准号：
10384063
负责人：
VANESSA SPERANDIO
金额：
$ 62.7万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-01-01 至 2027-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10384063
关键词：
2 year old 2-arachidonylglycerol Adrenergic Agents Animals Antibiotics Applications Grants Attenuated Beta-glucuronidase Biochemical Cannabidiol Cannabinoids Carbon Child Citrobacter rodentium Colitis Disease Down-Regulation Endocannabinoids Enterocytes Enzymes Epinephrine Epithelial Cells Equilibrium Escherichia coli Escherichia coli EHEC FDA approved Gene Expression Gene Expression Regulation Genes Genetic Glucuronates Goals Grant Hormones In Vitro Infection Instruction Intestines Isomerase Knock-out Knowledge Large Intestine Lesion Liposomes Maps Mediating Membrane Mus Neurotransmitters Norepinephrine Oxidopamine Pathogenesis Pathogenicity Island Pathway interactions Plants Principal Investigator Process Production Progress Reports Quantitative Reverse Transcriptase PCR Regulation Regulon Research Role Signal Transduction Source Stress Sugar Acids System Translating United States National Institutes of Health Virulence Western Blotting endogenous cannabinoid system experimental study microbiota mouse model mutant novel therapeutic intervention pathogen pharmacologic prevent programs protein expression protein-histidine kinase quorum sensing receptor reconstitution response transcriptomics uronate virulence gene

2 year old 2-arachidonylglycerol Adrenergic Agents Animals Antibiotics Applications Grants Attenuated Beta-glucuronidase Biochemical Cannabidiol Cannabinoids Carbon Child Citrobacter rodentium Colitis Disease Down-Regulation Endocannabinoids Enterocytes Enzymes Epinephrine Epithelial Cells Equilibrium Escherichia coli Escherichia coli EHEC FDA approved Gene Expression Gene Expression Regulation Genes Genetic Glucuronates Goals Grant Hormones In Vitro Infection Instruction Intestines Isomerase Knock-out Knowledge Large Intestine Lesion Liposomes Maps Mediating Membrane Mus Neurotransmitters Norepinephrine Oxidopamine Pathogenesis Pathogenicity Island Pathway interactions Plants Principal Investigator Process Production Progress Reports Quantitative Reverse Transcriptase PCR Regulation Regulon Research Role Signal Transduction Source Stress Sugar Acids System Translating United States National Institutes of Health Virulence Western Blotting endogenous cannabinoid system experimental study microbiota mouse model mutant novel therapeutic intervention pathogen pharmacologic prevent programs protein expression protein-histidine kinase quorum sensing receptor reconstitution response transcriptomics uronate virulence gene

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项目摘要

See continuation page 24 for project summary. Per NIH instructions, submit a one-page abstract of the research plan for the entire extension period (3-5 years) RELEVANCE (See instructions): The goal of this project is to investigate how different host neurotransmitters impact the balance among microbiota-pathogen-host interactions. We will assess how the stress hormones epinephrine and norepinephrine have their impact on enterohemorrhagic E. coli (EHEC) virulence gene regulation counteracted by an endocannabi noi d. We will also investigate how the microbiota alters the availability of the active form of these neurotransmitters in the gut.

有关项目摘要，请参见第24页。根据NIH说明，提交一页的摘要整个扩展期的研究计划（3 - 5年）相关性（请参阅说明）：该项目的目的是调查不同的宿主神经递质如何影响微生物群 - 病原体宿主相互作用。我们将评估应激激素肾上腺素和如何去甲肾上腺素对肠ho骨大肠杆菌（EHEC）毒力基因调控有影响由内源性noi d对抗。我们还将研究微生物群如何改变这些神经递质在肠道中的活性形式。