Supplement to NATIONAL CENTER FOR ADVANCED ESR TECHNOLOGY

国家先进 ESR 技术中心补充资料

• 批准号: 8417782
• 负责人: Jack H Freed
• 金额: $ 41.46万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8417782
• 关键词: Acquired Immunodeficiency Syndrome; Alzheimer' s Disease; Automobile Driving; Biological; Computer software; Depressive disorder; Detection; Disease; Electrons; Funding; Generations; Health; Heart Diseases; Hodgkin Disease; Hypersensitivity; Infection; Letters; Lung; Malignant Neoplasms; Measurement; Methodology; Methods; Monitor; Morphologic artifacts; National Center for Research Resources; Noise; Parkinson Disease; Performance; Phase; Physiologic pulse; Population; Positioning Attribute; Production; Proteins; Pseudomonas aeruginosa; Relaxation; Resolution; Sampling; Schizophrenia; Shapes; Signal Transduction; Structure; Techniques; Technology; Testing; United States National Institutes of Health; Virus Diseases; Width; base; combat; design; digital; improved; microwave electromagnetic radiation; nanosecond; nervous system disorder; new technology; novel; parent grant; protein complex; protein structure; quantum; research and development

DESCRIPTION (provided by applicant): This proposal is for a competitive revision, under NCRR funding announcement: PAR-12-046, of the parent grant R41RR016292 funding, P41 Center: National Biomedical Center for Advanced ESR Technology. This proposal is for a new technology project, not included on the parent grant, but which greatly benefits the aims of the parent grant, in particular Technology Research and Development (TR&D) Project: Structure of Proteins and Protein Complexes by Pulse Dipolar ESR (PDS-ESR), which is concerned with improving existing and developing new distance measurement techniques for structural studies on biomolecules. The new project is to develop methods of composite pulses, common in NMR, to be applied to ESR, which requires much shorter pulse widths and is subject to much faster spin-relaxation. This is now possible using a new Tektronix Arbitrary Waveform Generator (AWG) that will enable the modulation of the microwave pulses with sub-ns resolution. It will enable better spectral coverage by the pulses in the double-quantum coherence (DQC)-ESR method for distance measurements, which requires non-selective pulses. It will enable well-designed selective pulses required for the double electron-electron resonance (DEER) method. This will enhance signal-to-noise and suppression of unwanted signals and artifacts, thereby improving these methodologies. The AWG will be integrated into the pulse ESR spectrometer with due consideration of the letter's detailed performance. Monitoring of the composite pulsing at key positions in the microwave bridge, with the help of a sufficiently wide-bandwidth digital sampling oscilloscope (DSO), will permit automatic retuning of the AWG to modify as needed the composite pulses to guarantee the quality of the pulses that actually reach the sample. This project will involve a synergistic interaction, driven by the needs of the Driving Biomedical Projects (DBP's) as in the Parent Grant. Careful tests will be made of this new composite-pulse technology to demonstrate how it significantly improves the distance measurements and allows new and more challenging studies included in the DBP's to be carried out.

描述（由申请人提供）：本提案是根据 NCRR 资助公告：PAR-12-046，母基金 R41RR016292 资助的竞争性修订，P41 中心：国家先进 ESR 技术生物医学中心。该提案针对一项新技术项目，不包含在母基金中，但它极大地有利于母基金的目标，特别是技术研究与开发（TR＆D）项目：脉冲偶极ESR（PDS）的蛋白质和蛋白质复合物的结构-ESR），致力于改进现有的和开发用于生物分子结构研究的新距离测量技术。新项目旨在开发 NMR 中常见的复合脉冲方法，并将其应用于 ESR，这需要更短的脉冲宽度并且受到更快的自旋弛豫。现在可以使用新的泰克任意波形发生器 (AWG) 来实现这一点，该发生器将能够以亚纳秒分辨率调制微波脉冲。它将通过用于距离测量的双量子相干 (DQC)-ESR 方法中的脉冲实现更好的光谱覆盖，该方法需要非选择性脉冲。它将实现双电子-电子共振（DEER）方法所需的精心设计的选择性脉冲。 这将增强信噪比并抑制不需要的信号和伪影，从而改进这些方法。 AWG 将集成到脉冲 ESR 光谱仪中，并适当考虑字母的详细性能。在足够宽的带宽数字采样示波器 (DSO) 的帮助下，对微波桥中关键位置的复合脉冲进行监测，将允许 AWG 自动重新调谐，以根据需要修改复合脉冲，以保证脉冲的质量实际到达样品。该项目将涉及协同互动，由母基金中的驱动生物医学项目（DBP）的需求驱动。将对这种新的复合脉冲技术进行仔细测试，以证明它如何显着改进距离测量，并允许在 DBP 中进行新的、更具挑战性的研究。