Cell-cell Transmission of KSHV

KSHV 的细胞间传播

基本信息

批准号：
8375642
负责人：
TIMOTHY M ROSE
金额：
$ 26.46万
依托单位：
SEATTLE CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8375642
关键词：
AIDS related cancer AIDS/HIV problem Address Adhesions Adult Africa South of the Sahara Age Antibodies B-Cell Lymphomas B-Lymphocytes Biopsy CD209 gene Cell Adhesion Cell Adhesion Molecules Cell Culture Techniques Cell Differentiation process Cell Line Cell membrane Cell physiology Cells Cessation of life Child Childhood Coculture Techniques Core Facility Data Disease Epithelial Cells Epithelium Glycoproteins Goals Grant Growth Herpesviridae Herpesviridae Infections Human Human Herpesvirus 8 Immune Immune response Immunity Infection Instruction Integrin Binding Integrins Kaposi Sarcoma Laboratories Latent Virus Maintenance Malignant Neoplasms Mediating Methods Molecular Mouth Neoplasms Oral Oral cavity Palate Kaposi&apos s Sarcoma Parents Pathogenesis Pathology Pathway interactions Peptides Pharmaceutical Preparations Play Pleural effusion disorder Population Prevalence Procedures Process Production Property Proteins RGD (sequence)Reagent Recombinants Refractory Research Role Saliva Source System Testing Tissues Tonsil Training Viral Viral Pathogenesis Virion Virus Virus Latency cost immortalized cell in vivo latent infection mortality novel novel strategies pathogen programs receptor receptor expression recombinant virus therapeutic vaccine tissue/cell culture transmission process tumor viron

AIDS related cancer AIDS/HIV problem Address Adhesions Adult Africa South of the Sahara Age Antibodies B-Cell Lymphomas B-Lymphocytes Biopsy CD209 gene Cell Adhesion Cell Adhesion Molecules Cell Culture Techniques Cell Differentiation process Cell Line Cell membrane Cell physiology Cells Cessation of life Child Childhood Coculture Techniques Core Facility Data Disease Epithelial Cells Epithelium Glycoproteins Goals Grant Growth Herpesviridae Herpesviridae Infections Human Human Herpesvirus 8 Immune Immune response Immunity Infection Instruction Integrin Binding Integrins Kaposi Sarcoma Laboratories Latent Virus Maintenance Malignant Neoplasms Mediating Methods Molecular Mouth Neoplasms Oral Oral cavity Palate Kaposi&apos s Sarcoma Parents Pathogenesis Pathology Pathway interactions Peptides Pharmaceutical Preparations Play Pleural effusion disorder Population Prevalence Procedures Process Production Property Proteins RGD (sequence)Reagent Recombinants Refractory Research Role Saliva Source System Testing Tissues Tonsil Training Viral Viral Pathogenesis Virion Virus Virus Latency cost immortalized cell in vivo latent infection mortality novel novel strategies pathogen programs receptor receptor expression recombinant virus therapeutic vaccine tissue/cell culture transmission process tumor viron

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项目摘要

The main goal of the Cell Culture Core facility is to provide a stable, reproducible and reliable source of virus stocks and relevant KSHV-infected and uninfected primary and immortalized cell cultures for all of the projects involved in this program. This centralized source would be cost-saving and provide for reproducible experimentation across the different laboratories. The Core will support all four projects with the following aims 1) produce, isolate and titer stocks of KSHV and recombinant KSHV strains 2) grow, maintain and provide KSHV-infected and noninfected tissue culture cells 3) process tonsil and oral KS biopsies for tissue sectioning or primary cultures 4) provide training for virus production and purification, cell culture growth and maintenance and for biosafety methods and procedures for producing and utilizing herpesviruses in research.

细胞培养核心设施的主要目标是为参与该计划的所有项目提供稳定，可重复和可靠的病毒库存以及相关的KSHV感染和未感染的原始和不朽的细胞培养物。该集中式来源将省略成本，并为各个不同实验室提供可再现的实验。核心将以以下目的支持所有四个项目1）生产KSHV和重组KSHV菌株的生产，孤立和滴度库存2）生长，维护和提供受KSHV感染和未感染的组织培养细胞3）处理扁桃体和口服KS Biopies，用于生产和培训的生产和培训的培训4）研究中的疱疹病毒。