Mechanisms of fibrosis and lymphatic dysfunction in post-surgical lymphedema

术后淋巴水肿纤维化和淋巴功能障碍的机制

基本信息

批准号：
8532029
负责人：
Babak J Mehrara
金额：
$ 70.28万
依托单位：
SLOAN-KETTERING INST CAN RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-01 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8532029
关键词：
Adoptive Transfer American Anti-Inflammatory Agents Anti-inflammatory Antibodies Axillary Lymph Node Dissection Axillary lymph node group Bypass CD4 Positive T Lymphocytes Cancer Patient Chronic Clinical Clinical Research Clinical Trials Complication Congenital Abnormality Data Development Disease Down-Regulation Etiology Event Fibrosis Functional disorder IL4 gene Immunity Infection Infiltration Inflammation Inflammatory Injury Interleukin-13 Knowledge Laboratories Lead Liquid substance Lymph Lymphangiogenesis Lymphatic Lymphatic Endothelial Cells Lymphatic Metastasis Lymphatic System Lymphedema Mediating Medical Methods Mission Modeling Morbidity - disease rate Mus Natural regeneration Neoplasm Metastasis Obesity Obstruction Operative Surgical Procedures Organ Organ failure Palliative Care Pathology Patients Pharmaceutical Preparations Play Positioning Attribute Prevalence Prevention Proteins Quality of life Radiation Recurrence Recurrent pain Research Risk Role Sampling Secondary to Source Specimen Staging Surgical complication Swelling System T-Lymphocyte Tail Testing Th2 Cells Time Tissues Transgenic Mice United States National Institutes of Health Upper Extremity Vascular Endothelial Growth Factor C Work base cancer therapy clinical application clinically relevant clinically significant cytokine design high risk improved innovation lipid metabolism macrophage malignant breast neoplasm mouse model multidisciplinary novel palliative prevent repaired research study response therapy development tumor

Adoptive Transfer American Anti-Inflammatory Agents Anti-inflammatory Antibodies Axillary Lymph Node Dissection Axillary lymph node group Bypass CD4 Positive T Lymphocytes Cancer Patient Chronic Clinical Clinical Research Clinical Trials Complication Congenital Abnormality Data Development Disease Down-Regulation Etiology Event Fibrosis Functional disorder IL4 gene Immunity Infection Infiltration Inflammation Inflammatory Injury Interleukin-13 Knowledge Laboratories Lead Liquid substance Lymph Lymphangiogenesis Lymphatic Lymphatic Endothelial Cells Lymphatic Metastasis Lymphatic System Lymphedema Mediating Medical Methods Mission Modeling Morbidity - disease rate Mus Natural regeneration Neoplasm Metastasis Obesity Obstruction Operative Surgical Procedures Organ Organ failure Palliative Care Pathology Patients Pharmaceutical Preparations Play Positioning Attribute Prevalence Prevention Proteins Quality of life Radiation Recurrence Recurrent pain Research Risk Role Sampling Secondary to Source Specimen Staging Surgical complication Swelling System T-Lymphocyte Tail Testing Th2 Cells Time Tissues Transgenic Mice United States National Institutes of Health Upper Extremity Vascular Endothelial Growth Factor C Work base cancer therapy clinical application clinically relevant clinically significant cytokine design high risk improved innovation lipid metabolism macrophage malignant breast neoplasm mouse model multidisciplinary novel palliative prevent repaired research study response therapy development tumor

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项目摘要

DESCRIPTION (provided by applicant): Lymphedema is the accumulation of protein rich fluid in tissues, and in the US, occurs most commonly as a surgical complication of cancer treatment. Despite the fact that 3-5 million Americans suffer from lymphedema, its etiology is unknown and treatment remains palliative. Based on our laboratory data as well as the clinical presentation of lymphedema we hypothesize that the pathology of lymphedema is secondary to progressive fibrosis and is mediated by chronic T-helper (CD4+) cell inflammation. The objective of this proposal is to determine how lymphatic fluid stasis causes tissue fibrosis and lymphatic dysfunction. Our long-term objective is to develop therapies to treat or prevent lymphedema by disrupting the cycle of stasis, fibrosis, and lymphatic dysfunction. This approach is innovative since previous efforts have attempted to treat lymphedema by augmenting lymphatic repair and regeneration using cytokines. These cytokines, however, can also cause tumor metastasis or recurrence thereby limiting the applicability of this approach in cancer patients. This proposal is relevant since lymphedema is a common and morbid complication of cancer treatment for which there is no proven preventative methods and treatment is palliative. We plan to achieve our objectives using 3 specific aims. Aim 1: Determine the cellular sources of profibrotic cytokines. This aim will test the working hypothesis that CD4+ T-cells are the primary source of profibrotic cytokines using transgenic mice, antibody depletion, and adoptive transfer experiments in a mouse-tail and axillary dissection model. Aim 2: Determine how the expression of profibrotic cytokines is regulated. This aim will test the working hypothesis is that lymphatic stasis causes Th2 cell inflammation and IL4 and IL13 expression and that these cytokines interact to cause fibrosis and lymphatic dysfunction. We will use clinical specimens from patients with lymphedema before and after medical or surgical treatment as well as our mouse tail model. Aim 3: Determine how profibrotic cytokines regulate lymphatic function and fibrosis. This aim will test the working hypothesis that impaired lymphatic function secondary to fibrosis is a consequence of indirect effects of T-cells and Th2 cytokines on the lymphatic system rather than down-regulation of lymphangiogenic cytokine expression.

描述（由申请人提供）：淋巴水肿是组织中富含蛋白质的液体的积累，在美国，最常见的是癌症治疗的手术并发症。尽管有3-500万美国人患有淋巴水肿，但其病因尚不清楚，治疗仍然姑息治疗。根据我们的实验室数据以及淋巴水肿的临床表现，我们假设淋巴水肿的病理是进行性纤维化的继发的，并由慢性T-helper（CD4+）细胞炎症介导。该提案的目的是确定淋巴液的停滞如何引起组织纤维化和淋巴功能障碍。我们的长期目标是开发疗法来通过破坏暂停，纤维化和淋巴功能障碍的周期来治疗或预防淋巴水肿。这种方法是创新的，因为以前的努力试图通过使用细胞因子来增强淋巴修复和再生来治疗淋巴水肿。但是，这些细胞因子也可能引起肿瘤转移或复发，从而限制了这种方法在癌症患者中的适用性。该提议是相关性，因为淋巴水肿是癌症治疗中常见且病态的并发症，没有证实的预防方法和治疗是姑息治疗的。我们计划使用3个特定目标来实现我们的目标。目标1：确定纤维化细胞因子的细胞来源。该目标将检验以下假设：CD4+ T细胞是使用转基因小鼠，抗体耗竭和小鼠尾和腋窝扫描模型中的抗纤维细胞因子的主要来源。 AIM 2：确定如何调节纤维化细胞因子的表达。该目的将检验工作假设是淋巴停滞会引起Th2细胞炎症，IL4和IL13表达，并且这些细胞因子相互作用以引起纤维化和淋巴功能障碍。我们将使用医疗或手术治疗前后淋巴水肿的患者以及小鼠尾部模型的临床标本。 AIM 3：确定纤维化细胞因子如何调节淋巴功能和纤维化。该目标将检验以下假设：纤维化继发淋巴功能受损是T细胞和Th2细胞因子对淋巴系统的间接作用的结果，而不是淋巴管植物细胞因子表达的下调。