The Norwegian Mother And Child Study: Environmental Specimen Collection

挪威母子研究：环境样本收集

基本信息

批准号：
8734070
负责人：
Matthew Longnecker
金额：
$ 68.76万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8734070
关键词：
Autistic Disorder Biological Birth Blood C-reactive protein Cerebral Palsy Child Childhood Clinical Chemistry Cohort Studies Collection Data Data Collection Development Dietary intake Disease Drug Kinetics Enrollment Environmental Exposure Epidemiology Fathers Finding by Cause Future Genes Gestational Diabetes Growth Hand Health Human Infant Infection Laboratories Learning Measures Medical Metabolic Mothers National Institute of Environmental Health Sciences Organophosphates Outcome Paper Pesticides Physical activity Plasma Pre-Eclampsia Predisposition Pregnancy Protocols documentation Publishing Questionnaires Reporting Research Personnel Risk Risk Factors Scientist Series Serum Specimen System Testing Trace Elements Urine Whole Blood Woman Women&apos s Group Work base bisphenol A cohort disorder risk in utero inflammatory marker insight interest neurodevelopment pesticide exposure phthalates sample collection transmission process

项目摘要

The Norwegian Mother and Child Cohort Study (MoBa) is an ongoing prebirth cohort study. Enrollment was completed in 2008 resulting in 106,980 pregnancies in the study. The study is based on questionnaires completed by the mother and father, and biological specimens are collected from the mother, father and child. The main purpose of the study is to find causes of diseases. NIEHS is providing partial support for the study, and in 2002 expanded the data collection protocol to include collection of more biologic specimens from the mothers during the 17th week of pregnancy (additional serum, a specimen of whole blood collected in a trace-element free container, and urine). These specimens will enhance the ability to examine the relation of environmental exposures in relation to various health outcomes in the children and their mothers. As of April 30, 2009, 77,104 subjects had provided blood and urine that was collected in conjunction with NIEHS. We added to the MoBa protocol the collection additional urine specimens in pregnancy, at weeks 23 and 29, for a subset of women. 690 women have a complete set of three urine specimens from pregnancy. This year the laboratory work was completed for a study in which we attempted to measure the levels of metabolites of organophosphate pesticides and bisphenol A in these specimens, to determine how reproducibile the levels were. We have learned that the preservative used in the collection of the urine specimens contaminated them with free bisphenol A, and makes the specimens difficult to analyze for organophosphate pesticide metabolites. This information will be of great use as we plan future studies. Future studies seeking to quantitate bisphenol A will need to measure total and free BPA, so that conjuated BPA can be estimated. Special new studies will be needed to see if the specimens can be analyzed for organophosphate pesticide metabolites, because the standard approach will not work. We published one paper reporting the contamination, and have identified two laboratories that will work with the urine specimens to see if they can analyze them for organophosphate pesticide metabolites and phthalates. We also have studied the plasma concentration of perfluoralkyl substances in relation to various outcomes among women in the MoBa cohort. This year we published one paper on the determinants of plasma levels of these compounds. We are completing a series of other papers based on our data on plasma levels of the compounds; one is on preeclampsia, two are on values of clinical chemistry parameters in relation to perfluorinated compound levels, and one, using the same group of women, is about physical activity in pregnancy and plasma levels of C-reactive protein, a marker of inflammation. We are planning a series of new studies, using the data in hand, to examine a) the reliability of the plasma levels over a period of several years, b) exposure in relation to child growth, and c) exposure in relation to infections in childhood. Because of our interest in perfluoroalkyl substances, we have also been collaborating on studies of the pharmacokinetics of these compounds, which provide insight into epidemiologic findings. We published one paper on the pharmacokinetics of these compounds in the past year. Scientists at NIEHS are developing several studies that will be nested within MoBa. A list of the studies currently planned includes: Risk factors for cerebral palsy, A Search for the Non-mendelian Transmission of Human Genes, Dietary Intake and Gestational Diabetes, Physical Activity in Pregnancy and Infant Neurodevelopment, Organophosphate Pesticide Exposure and Risk of Autism, and additional studies on perfluorinated compound exposure and health.

挪威母婴队列研究 (MoBa) 是一项正在进行的产前队列研究。该研究于 2008 年完成入组，共有 106,980 名孕妇怀孕。该研究以母亲和父亲填写的问卷为基础，并从母亲、父亲和孩子身上采集生物样本。研究的主要目的是寻找疾病的原因。 NIEHS 为这项研究提供部分支持，并于 2002 年扩大了数据收集方案，包括在怀孕第 17 周期间从母亲身上收集更多的生物样本（额外的血清，即在无微量元素的容器中收集的全血样本）和尿液）。这些标本将增强检查环境暴露与儿童及其母亲的各种健康结果之间关系的能力。截至 2009 年 4 月 30 日，已有 77,104 名受试者提供了与 NIEHS 联合收集的血液和尿液。我们在 MoBa 方案中添加了在怀孕第 23 周和第 29 周时为一部分女性收集额外尿液样本的内容。 690 名女性拥有一套完整的三份怀孕尿液样本。今年，我们完成了一项研究的实验室工作，其中我们试图测量这些样本中有机磷农药和双酚 A 代谢物的水平，以确定这些水平的重现性。我们了解到，收集尿液样本时使用的防腐剂使样本受到游离双酚A的污染，导致样本难以分析有机磷农药代谢物。当我们计划未来的研究时，这些信息将非常有用。未来寻求定量双酚 A 的研究将需要测量总 BPA 和游离 BPA，以便估算共轭 BPA。需要进行专门的新研究来确定是否可以分析样本中的有机磷农药代谢物，因为标准方法行不通。我们发表了一篇报告污染情况的论文，并确定了两个实验室将处理尿液样本，看看是否可以分析其中的有机磷农药代谢物和邻苯二甲酸盐。我们还研究了全氟烷基物质的血浆浓度与 MoBa 队列中女性各种结果的关系。今年，我们发表了一篇关于这些化合物血浆水平的决定因素的论文。我们正在根据化合物血浆水平的数据完成一系列其他论文；一项是关于先兆子痫，两项是关于与全氟化合物水平相关的临床化学参数值，一项是关于怀孕期间的体力活动和 C 反应蛋白（炎症标志物）的血浆水平，使用同一组妇女。我们正在计划利用现有数据进行一系列新研究，以检查 a) 几年内血浆水平的可靠性，b) 暴露与儿童生长的关系，以及 c) 暴露与感染的关系。童年。由于我们对全氟烷基物质的兴趣，我们还一直在合作研究这些化合物的药代动力学，从而深入了解流行病学发现。去年我们发表了一篇关于这些化合物的药代动力学的论文。 NIEHS 的科学家正在开展几项将嵌套在 MoBa 中的研究。目前计划的研究清单包括：脑瘫的危险因素、寻找人类基因的非孟德尔传播、膳食摄入和妊娠糖尿病、妊娠期体力活动和婴儿神经发育、有机磷农药暴露和自闭症风险，以及关于全氟化合物暴露与健康的其他研究。