Equipment for Gross Hall Vivarium

Gross Hall 动物园设备

• 批准号: 8526261
• 负责人: Jeffrey Goodwin
• 金额: $ 33.43万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-01 至 2014-10-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8526261
• 关键词: Address; Air; Alzheimer' s Disease; Alzheimer' s disease model; Animal Experimentation; Animals; Behavior; Biological; Biological Sciences; Blindness; Breeding; Budgets; California; Certification; Complex; Diabetes Mellitus; Equipment; Exclusion; Funding; Genetic; Grant; Growth; Health Sciences; Heating; Housing; Immunology; Institution; Knock-in Mouse; Letters; Location; Macular degeneration; Malignant Neoplasms; Mediation; Metabolic Diseases; Mouse Strains; Mus; Neurobiology; Neurosciences Research; Platinum; Procedures; Regenerative Medicine; Request for Applications; Research; Research Personnel; Retinitis Pigmentosa; Safety; Services; Side; Site; Spinal cord injury; Stroke; Transgenes; United States National Institutes of Health; Universities; Veterinarians; animal facility; animal resource; biosecurity; cost; design and construction; embryo cell; neuropathology; operation; pathogen; professor; programs; research facility; square foot; tau Proteins; transgenic model of alzheimer disease; Address; Air; Alzheimer' s Disease; Alzheimer' s disease model; Animal Experimentation; Animals; Behavior; Biological; Biological Sciences; Blindness; Breeding; Budgets; California; Certification; Complex; Diabetes Mellitus; Equipment; Exclusion; Funding; Genetic; Grant; Growth; Health Sciences; Heating; Housing; Immunology; Institution; Knock-in Mouse; Letters; Location; Macular degeneration; Malignant Neoplasms; Mediation; Metabolic Diseases; Mouse Strains; Mus; Neurobiology; Neurosciences Research; Platinum; Procedures; Regenerative Medicine; Request for Applications; Research; Research Personnel; Retinitis Pigmentosa; Safety; Services; Side; Site; Spinal cord injury; Stroke; Transgenes; United States National Institutes of Health; Universities; Veterinarians; animal facility; animal resource; biosecurity; cost; design and construction; embryo cell; neuropathology; operation; pathogen; professor; programs; research facility; square foot; tau Proteins; transgenic model of alzheimer disease

DESCRIPTION (provided by applicant): This application requests funds to equip a Breeding Center in holding space in a new facility, Gross Hall Animal Resource Center (GHARC), funded by an HHS CO6 ARRA grant NIH Grant no. 1C06RR030131-01 and in support of a group of NIH-funded researchers at the University of California, Irvine (UCI). The project would achieve specific aims for the UCI animal program, and addresses deficiencies in the GHARC as currently budgeted. The proposed Breeding Center would consist of two holding suites, each 40'x23' = 920 sf x 2 suites = 1840 sf total, and would be located inside the barrier in the Gross Hall Animal Resource Center (GHARC), which occupies 17,433 net assignable square feet (23,385 gross square feet) in the basement of Sue and Bill Gross Hall, the newest research building on the UCI campus. The Breeding Center will support breeding and holding of mice, including valuable genetically modified mice. One example: to better model Alzheimer's Disease (AD) neuropathology, Frank LaFerla, Chancellor's Professor of Neurobiology and Behavior, created a triple transgenic model of AD (3xTg-AD). Rather than crossing independent lines, LaFerla microinjected two transgenes (ssAPP and tau) into single- cell embryos from homozygous PS1M146V knockin mice, generating mice with the same genetic background. These mice are used extensively in AD research and exported to other research institutions. At UCI, valuable genetically altered mouse breeders such as the 3xTg-AD are housed in Breeding Centers using strict biosecurity standards: broad pathogen exclusion list, mandatory rederivation, limited research staff access, appropriate personal protective equipment (PPE), etc. The two Breeding Center holding/procedure suites consist of an Anteroom, Procedure room, and two Holding rooms. Requested equipment includes twelve (12) double sided cage racks (140 cages/rack), four (4) animal transfer stations, and two (2) Class 2 Type A biological safety cabinets. At UCI, valuable genetically altered mouse line founders are housed whenever possible in specialized Breeding Centers-McGaugh Hall in Biological Sciences and Hewitt Hall in the BRC. These Breeding Centers use strict biosecurity standards: broad pathogen exclusion list, mandatory rederivation, limited research staff access, and appropriate PPE. With the addition of capacity for 7,280 mouse cages in the GHARC, the single Breeding Center in Hewitt Hall is insufficient to provide experimental mice for the Gross-Gillespie-Hewitt BRC complex. The Breeding Center in the GHARC will support 18 researchers with NIH funding in excess of $10.8 million, and $4.9 million pending. The caging and equipment will support primarily breeding stock used by a group of PHS-funded basic and translational researchers in regenerative medicine, Alzheimer's disease, cancer, diabetes and other metabolic disorders, macular degeneration, retinitis pigmentosa, herpes-related blindness, immunology, spinal cord injury, and stroke. Gross Hall, site of the GHARC and proposed Breeding Center, has received LEED Platinum certification, and the proposed Breeding Center Facility would use "green" cage racks (63% more energy efficient); Variable Air Volume (VAV) room HVAC, the campus's first in a vivarium; and an efficient dry heat sterilizer.

描述（由申请人提供）：本申请要求资金在新设施的持有空间中装备一个由HHS CO6 ARRA ARRA GRANT NIH NIH GRANT NO. 1C06RR030131-01并支持加利福尼亚大学尔湾分校（UCI）的一群NIH资助的研究人员。该项目将针对UCI动物计划实现特定的目标，并解决当前预算的GHARC中的缺陷。拟议的育种中心将由两个套件组成，每个套件= 920平方英尺x 2套件= 1840平方英尺，总共将位于障碍物中的大厅动物资源中心（GHARC）的障碍物内，该障碍物占据了17,433个可分配的平方英尺（23,385 GROSS ASSEMENT ASSEMENT SUE和BILL GROSS GROSS HALL，NOWS -NONES -NOWS -NOWS -CREENS rock，in News -News subsement of Sue and -Nestable subsement of Sue and -Nestable Assement of Suement of Subsement of。繁殖中心将支持小鼠的繁殖和持有，包括有价值的遗传改性小鼠。一个例子：更好地模拟阿尔茨海默氏病（AD）神经病理学，弗兰克·拉弗拉（Frank Laferla），总理的神经生物学和行为教授弗兰克·拉弗拉（Frank Laferla）创建了AD的三重转基因模型（3XTG-AD）。 Laferla微型注射并没有越过独立的线，而是将两个转基因（SSAPP和TAU）从纯合PS1M146V敲击蛋白小鼠中产生到单细胞胚胎中，从而产生具有相同遗传背景的小鼠。这些小鼠广泛用于AD研究中，并导出到其他研究机构。在UCI，使用严格的生物安全标准将有价值的遗传变化的老鼠育种者（例如3XTG-AD）安置在育种中心中：广泛的病原体排除清单，强制性重新培训，有限的研究人员访问，适当的个人保护设备（PPE）等。两个由两个育种中心套件组成的饲养套装，由两个育种中心套装组成。请求的设备包括十二（12）个双面笼架（140个笼子/机架），四（4）个动物转移站和两个（2）级A类A型生物安全柜。在UCI，在BRC的生物科学和Hewitt Hall的专业育种中心，在可能的遗传上改变了遗传变化的小鼠线创始人。这些育种中心使用严格的生物安全标准：广泛的病原体排除清单，强制性重新培训，有限的研究人员访问和适当的PPE。随着在GHARC中增加7,280个小鼠笼的能力，休伊特大厅的单个繁殖中心不足以为总gillespie-gillespie-hewitt BRC综合体提供实验性小鼠。 GHARC的繁殖中心将为18名NIH资金超过1080万美元和490万美元的NIH资金提供支持。笼子和设备将支持一组由PHS资助的基本和翻译研究人员使用的繁殖库存，阿尔茨海默氏病，癌症，糖尿病和其他代谢性疾病，黄斑变性，色素性视网膜炎，疱疹相关，疱疹相关的盲，免疫学，脊柱损伤和stroke和Streoke和Streoke。 GROSS Hall是GHARC和拟议的育种中心的地点，已获得LEED白金认证，拟议的育种中心设施将使用“绿色”笼架（能源效率高63％）；可变空气体积（VAV）室HVAC，校园在Vivarium中的第一个；以及有效的干热灭菌剂。