Nanotechnology for Minimally Invasive Cancer Detection and Resection

用于微创癌症检测和切除的纳米技术

• 批准号: 8456176
• 负责人: Aaron M. Mohs
• 金额: $ 22.82万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8456176
• 关键词: Address; Animal Model; Animals; Artificial nanoparticles; Award; Benign; Biomedical Engineering; Caliber; Cancer Center; Cancer Detection; Cancer Patient; Cancerous; Canis familiaris; Clinical; Computers and Advanced Instrumentation; Contrast Media; Detection; Development; Devices; Diagnostic; Disease; Doctor of Medicine; Doctor of Philosophy; Drug Kinetics; Endoscopes; Endoscopy; Engineering; Environment; Excision; FDA approved; Faculty; Feedback; Fiber Optics; Fluorescence; Fluorescent Dyes; Foundations; Goals; Hemorrhage; Image; Indocyanine Green; Injury; Institution; International; K-Series Research Career Programs; Laboratory Research; Laparoscopic Surgical Procedures; Lead; Malignant - descriptor; Malignant Neoplasms; Medical; Medicine; Mentors; Mentorship; Methods; Modeling; Morbidity - disease rate; Nanotechnology; Nodule; Non-Small-Cell Lung Carcinoma; Normal tissue morphology; Operating Rooms; Operative Surgical Procedures; Optics; Pain; Pathological Staging; Pathology; Pathway interactions; Patients; Penetration; Pennsylvania; Phase; Polymers; Positioning Attribute; Primary Neoplasm; Procedures; Quality of life; Recovery; Recurrence; Recurrent tumor; Repeat Surgery; Research; Research Methodology; Research Personnel; Resected; Residual Cancers; Residual Tumors; Science; Sensitivity and Specificity; Spectrum Analysis; Surgeon; Surgical Oncology; Surgical incisions; Surgical margins; System; Techniques; Thoracic Neoplasms; Thoracic Surgical Procedures; Time; Tissues; Training; Tumor Tissue; Universities; Validation; abstracting; animal tissue; base; biocompatible polymer; biodegradable polymer; cancer surgery; career; clinical efficacy; clinically relevant; design; disability; flexibility; fluorophore; high risk; improved; innovation; instrumentation; intraoperative imaging; meetings; miniaturize; minimally invasive; mortality; nano; nanoparticle; nanoprobe; neoplastic cell; optical fiber; physical property; programs; self assembly; tumor

NANOTECHNOLOGY FOR MINIMALLY INVASIVE CANCER DETECTION AND RESECTION Project Abstract Effective surgical resection of tumors is the most important predictor for cancer patient survival. Although surgery is curative in approximately 45% of cancer patients, up to 40% of patients have recurrent tumors due to undetectable differences between malignant and benign hyperplasic or normal tissue, leading to incomplete resection of cancerous tissue. In addition, patients that undergo surgery often suffer a decreased quality of life due to injury associated with the surgery. The primary goal of this Pathway to Independence Award in Cancer Nanotechnology Research (K99/R00) proposal is to integrate the unique capabilities of nanotechnology with innovative optical instrumentation to improve detection and resection of malignant tissue through minimally invasive surgery. This challenge will be addressed by combining expertise and research methodology in nanotechnology, instrumentation, and surgical oncology. This career development award has four specific aims: (1) develop biodegradable and nontoxic activatable fluorescence nanoparticle probes; (2) develop a miniaturized and flexible device for intraoperative fluorescence detection; (3) integrate the miniaturized, flexible optical device with endoscopy for minimally invasive detection of tumors; and (4) evaluate the spectral endoscope using spontaneous thoracic tumors in large animals (canines) during surgery to improve disease clearance and pathological staging. Accomplishing these specific aims will utilize targeted and activatable nanoparticles to increase specific localization of the probes in cancerous tissue. Detecting and resecting cancerous tissue via the fiber optic endoscopic imaging system will decrease the rate of tumor recurrence by more accurately detecting surgical margins and residual cancer and reduce surgery associated morbidity, such as decreasing patient pain, discomfort, and disability. My immediate career goal is to obtain a tenure-track faculty position that focuses on integrating nanotechnology with surgical oncology. Long-term, I would like to lead a research program at the interface of science, medicine, and engineering and expand the number and types of diseases that will be investigated. Ideally this research would be performed at an institution where I can be involved with academic and medical investigators from diverse fields. Training during the mentored phase of this award will focus on several key aspects to facilitate my development to achieve these goals as an independent investigator, including (1) providing the candidate with a strong foundation in optical nanoparticle engineering, (2) instrumentation for fiber optic based spectral and near-infrared imaging, and (3) methodological challenges to minimally invasive laparoscopic procedures in surgical oncology. Training will take place in the Emory-Georgia Tech Biomedical Engineering Department under the mentorship of Dr. Shuming Nie, Ph.D., an international expert in nanotechnology and director of the Emory-Georgia Tech Center for Cancer Nanotechnology Excellence, and at the University of Pennsylvania under the co-mentorship of Dr. Sunil Singhal, M.D., Director of the Thoracic Surgery Research Laboratory and Chief of Thoracic Surgery. The environment at these two institutions is ideal for this project because I will have full access to the most advanced instrumentation for nanoparticle design, synthesis, and characterization; I will benefit from instrumentation engineers with fabrication facilities to meet my needs; and a highly collaborative translational environment, which is paramount for successful development of this project that integrates nanotechnology with minimally invasive intraoperative instrumentation. In addition, the collaborative training will be supplemented by formal coursework at Emory and Georgia Tech in optics and instrumentation.

微创癌症检测和切除的纳米技术 项目摘要 肿瘤的有效手术切除是癌症患者生存的最重要预测因子。虽然 在大约45％的癌症患者中，手术是治愈性的，多达40％的患者患有复发性肿瘤 由于恶性和良性增生或正常组织之间无法检测到的差异，导致不完整 切除癌组织。此外，接受手术的患者经常遭受生活质量的降低 由于与手术有关的伤害。这一获得癌症独立奖的主要目标 纳米技术研究（K99/R00）的建议是将纳米技术的独特功能与 创新的光学仪器，以最少的方式改善对恶性组织的检测和切除 侵入性手术。通过将专业知识和研究方法结合在一起，将解决这一挑战 纳米技术，仪器和外科肿瘤学。该职业发展奖有四个特定 目的：（1）开发可生物降解和无毒的荧光纳米粒子探针； （2）开发 微型和柔性装置，用于术中荧光检测； （3）整合微型，灵活的 具有内窥镜检查的光学装置，可微创检测肿瘤； （4）评估光谱 内窥镜在手术期间使用大型动物（犬）中自发性胸部肿瘤以改善疾病 清除和病理分期。完成这些特定目标将利用目标和可激活 纳米颗粒增加癌组织中探针的特定定位。检测和切除 通过光纤内窥镜成像系统癌组织将降低肿瘤复发率 通过更准确地检测手术边缘和残留癌症并减少相关的手术 发病率，例如减轻患者疼痛，不适和残疾。我的直接职业目标是获得 终身教师职位的重点是将纳米技术与外科肿瘤学相结合。长期，我 想在科学，医学和工程的界面领导研究计划，并扩展 将研究的疾病数量和类型。理想情况下，这项研究将在 我可以参与来自不同领域的学术和医学调查人员的机构。培训期间 该奖项的指导阶段将重点关注几个关键方面，以促进我的发展以实现 作为独立调查员的这些目标，包括（1）为候选人提供强大的基础 光学纳米颗粒工程，（2）基于光纤的光谱和近红外成像的仪器， （3）手术肿瘤学中最小侵入性腹腔镜程序的方法论挑战。 培训将在指导下在Emory-Georgia Tech生物医学工程部门进行 Shuming Nie博士博士，纳米技术的国际专家，Emory-Georgia Tech的主任 癌症纳米技术中心卓越，并在宾夕法尼亚大学的会议下 医学博士Sunil Singhal博士，胸外科研究实验室主任兼胸腔主管 外科手术。这两个机构的环境是该项目的理想选择，因为我将完全访问 纳米颗粒设计，合成和表征的最先进的仪器；我会从中受益 具有制造设施的仪器工程师以满足我的需求；以及高度协作的翻译 环境，这对于整合纳米技术的成功开发至关重要 具有最小侵入性术中仪器。此外，协作培训将是 在Emory和Georgia Tech的光学和仪器方面的正式课程补充。