Detection of Carcinomas

癌症检测

基本信息

批准号：
8459519
负责人：
Xiaojing Zhang
金额：
$ 29.11万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8459519
关键词：
Appearance Automation Biological Assay Biopsy Blood Blood Circulation Blood Tests Breast Breast Cancer Cell Breast Carcinoma Carcinoma Cells Clinical Pathology Detection Development Devices Diagnosis Early Diagnosis Ensure Evaluation FDA approved Goals Growth Hour Image Laboratories Magnetism Malignant - descriptor Malignant Neoplasms Measures Methods Microfluidics Microscope Microscopic Microscopy Mutation Neoplasm Circulating Cells Neoplasm Metastasis Non-Malignant Organ Pathologist Patients Pattern Physicians Research Sensitivity and Specificity Solid Neoplasm Staging Technology Time Tissues Treatment Effectiveness Tumor Markers anticancer research base cancer cell cancer diagnosis disease diagnosis improved innovation instrument instrumentation malignant breast neoplasm microchip nanomagnetic nanoparticle nanoscale neoplastic cell novel outcome forecast particle programs prototype public health relevance screening tumor

项目摘要

DESCRIPTION (provided by applicant): The long term objective of this research is to diagnose cancer via a blood test at an earlier stage and thereby increase the cure rate. We will focus in this program on developing our two instruments to follow genetic changes associated with progressive carcinoma and thereby, eventually help to personalize therapy. It is known that malignant solid tumors shed tumor cells into the circulation at a relatively early stage in their growth. Therefore, if it were possible to capture these relatively rare cells (CTCs) and prove that they are malignant, earlier diagnosis would be expected. Microchips that capture CTCs with great sensitivity have been recently described. We have made such a microchip with proven sensitivity but have altered it using immunolablled nanomagnetic particles for capturing CTCs, which can then be released to an innovative hyperspectral imaging microscope (HMI) that can precisely quantify 10 tumor markers (TMs) in a single pass. The proposed CTC chip-HMI platform can find tumor markers in blood in less than an hour, and with 10-fold greater sensitivity and specificity then existing (commercial) devices such as Cell Tracks, an FDA approved instrument for determining prognosis and treatment effectiveness in breast cancer based on a very small number of captured CTCs. A major breakthrough is the demonstration that over expression of TMs by cancer breast cells allows them to be distinguished from normal breast cells which have consistently less over expression of TMs. Therefore, after capture of the CTCs, evaluation of their TM expression by the HMI and the pathologist's conclusion concerning malignancy, we can then allow for the development of criteria for the number and extent of TM over expression that minimizes false positives but not at the expense of increasing false negatives. The extent and patterns of over expression of particular TMs will also be of considerable value for prognosis and treatment decisions. We will study breast carcinoma as a prototype of other carcinomas, primarily due to that the malignant tumors can be precisely measured radiographically and we have a large supply of patients. We plan to focus on the ability of our instrumentation to find CTCs in patients with small breast carcinomas.

描述（由申请人提供）：这项研究的长期目标是通过早期血液检查诊断癌症，从而提高治愈率。在这个项目中，我们将重点开发两种仪器来跟踪与进展性癌症相关的基因变化，从而最终帮助个性化治疗。众所周知，恶性实体瘤在其生长的相对早期阶段将肿瘤细胞释放到循环中。因此，如果能够捕获这些相对稀有的细胞（CTC）并证明它们是恶性的，则有望实现早期诊断。最近描述了能够以高灵敏度捕获 CTC 的微芯片。我们已经制造了这种具有经过验证的灵敏度的微芯片，但使用免疫标记的纳米磁性颗粒对其进行了更改，以捕获 CTC，然后将其释放到创新的高光谱成像显微镜 (HMI)，该显微镜可以一次性精确量化 10 种肿瘤标志物 (TM)。拟议的 CTC 芯片-HMI 平台可以在不到一小时的时间内找到血液中的肿瘤标志物，其灵敏度和特异性比现有（商业）设备（例如 Cell Tracks）高 10 倍，Cell Tracks 是 FDA 批准的一种仪器，用于确定癌症患者的预后和治疗效果。基于极少数捕获的 CTC 的乳腺癌。一项重大突破是证明乳腺癌细胞过度表达TMs，使其能够与正常乳腺细胞区分开来，而正常乳腺细胞的TMs过度表达始终较少。因此，在捕获 CTC、通过 HMI 评估其 TM 表达以及病理学家关于恶性肿瘤的结论后，我们可以制定 TM 过度表达的数量和程度的标准，以最大限度地减少假阳性，但不会以牺牲增加假阴性。特定 TM 过度表达的程度和模式对于预后和治疗决策也具有相当大的价值。我们将把乳腺癌作为其他癌症的原型来研究，主要是因为恶性肿瘤可以通过放射学精确测量，而且我们有大量的患者供应。我们计划重点关注我们的仪器在小乳腺癌患者中发现 CTC 的能力。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Emerging micro- and nanotechnologies in cancer diagnosis and therapy.

癌症诊断和治疗中的新兴微米和纳米技术。

DOI：
10.1007/s10544-013-9785-5
发表时间：
2013
期刊：
Biomedical microdevices
影响因子：
2.8
作者：
Zhang,JohnXJ;Khademhosseini,Ali
通讯作者：
Khademhosseini,Ali

Advances in liquid biopsy on-chip for cancer management: Technologies, biomarkers, and clinical analysis.

DOI：
10.1080/10408363.2018.1425976
发表时间：
2018-05
期刊：
Critical reviews in clinical laboratory sciences
影响因子：
10
作者：
Tadimety A;Closson A;Li C;Yi S;Shen T;Zhang JXJ
通讯作者：
Zhang JXJ

Microchip-based immunomagnetic detection of circulating tumor cells.

DOI：
10.1039/c1lc20270g
发表时间：
2011-10-21
期刊：
Lab on a chip
影响因子：
6.1
作者：
Hoshino K;Huang YY;Lane N;Huebschman M;Uhr JW;Frenkel EP;Zhang X
通讯作者：
Zhang X

Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system.

DOI：
10.1007/s10544-012-9718-8
发表时间：
2013-08
期刊：
BIOMEDICAL MICRODEVICES
影响因子：
2.8
作者：
Huang, Yu-yen;Hoshino, Kazunori;Chen, Peng;Wu, Chun-hsien;Lane, Nancy;Huebschman, Michael;Liu, Huaying;Sokolov, Konstantin;Uhr, Jonathan W.;Frenkel, Eugene P.;Zhang, John X. J.
通讯作者：
Zhang, John X. J.

Multi-Dimensional Nanostructures for Microfluidic Screening of Biomarkers: From Molecular Separation to Cancer Cell Detection.