InstaCortisol:A realtime and continuous assessment of Cortisol in ISF

InstaCortisol：ISF 中皮质醇的实时、连续评估

基本信息

批准号：
7992743
负责人：
Manju Grover Venugopal
金额：
$ 0.67万
依托单位：
GUIDED THERAPEUTICS
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-06-01 至 2010-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7992743
关键词：
Acquired Immunodeficiency Syndrome Adrenal Cortex Age Alkaline Phosphatase Antibodies Antineoplastic Agents Behavior Behavioral Sciences Benchmarking Biological Assay Biological Markers Biological Process Biosensor Calibration Categories Clinical Data Data Collection Detection Devices Diabetes Mellitus Diet Digestion Disease Drug Addiction Elderly Environment Enzymes Epinephrine Error Sources Etiology Evaluation Exercise Exposure to Frequencies Genetic Hand Harvest Healed Health Heart Diseases Hormones Hour Household Housing Human Hydrocortisone Hypertension Immune system Individual Intercellular Fluid Intervention Learning Life Life Style Major Depressive Disorder Malignant Neoplasms Marketing Measurement Measures Mental Depression Metabolism Methods Monitor Muscle Natural regeneration Nicotine Normal Cell Obesity Osteoporosis Participant Patient Self-Report Patients Pattern Performance Phase Physical environment Physiological Play Process Production Psychosocial Stress Public Health Reaction Research Research Personnel Role Salivary Sampling Sensitivity and Specificity Signal Transduction Sleep Smoker Social Interaction Socioeconomic Status Sports Medicine Steroids Stress Surveys System Technology Testing Time Weight Gain Wireless Technology Wrist base bone cross reactivity dehydroepiandrosterone design detector experience field study healing human disease improved innovation meetings minimally invasive operation physical conditioning psychosocial public health relevance research study sensor strength training stressor transmission process virtual

项目摘要

DESCRIPTION (provided by applicant): "StressSmart" Repeated exposures to psychosocial stress are associated with several human diseases (e.g., cancer, drug addiction, heart disease, depression) of tremendous public health burden. However, our progress is limited by the lack of a portable device that can enable rapid, reliable, non-invasive and continuous measurement of personal exposure to psychosocial stress in real-time, as experienced by individuals in their natural environments. In this interdisciplinary project, we propose to develop a new test called InstaCortisol(tm) that can be used to reliably, selectively and rapidly quantify personal exposures to stress by measuring Cortisol levels of subjects in their natural environments. Cortisol is the primary steroid produced and secreted by the adrenal cortex and is used as a reliable indicator of stress. This rapid test is based on immunoelectrochemical method where the reaction signal is measured by using cyclic voltammetry, when Cortisol sandwiches between the capture and the detector antibody, which is attached to Alkaline Phosphatase enzyme. There are four specific aims of this project. First is Sensor Fabrication: We will fabricate multiple Cortisol sensors in first two months. Second is Sensor Evaluation: we will test the sensors for sensitivity, specificity, enzyme/antibody stability, detection limit, linearity, accuracy, dynamic range, repeatability, precision, accuracy, interference, cross-reactivity, drift, and sensor calibration. We anticipate changing and/or optimizing fabrication methods to achieve benchmarks in each of these categories. Third is sensor performance: Establish sensor performance against commercially available Cortisol sensors. We aim to meet or exceed, given the need for continuous performance benchmarks of readily available Cortisol sensors. Fourth would be to Design integration feasibility experiments: A virtual evaluation of Cortisol sensor chip and its integration into a compact easy to use design. Relevance: The InstaCortisol(tm) test (being developed during Phase I), which will be part of StressSmart(tm) device during Phase II, will enable researchers to quantify personal exposure to psychosocial stress, as experienced by individuals in real-life situations. Such a capability will advance our understanding of how exposures to psychosocial stress interact with genetic factors in the etiology of prevalent human diseases of tremendous public health burden. PUBLIC HEALTH RELEVANCE: We are proposing to make a rapid test for detecting Cortisol, called "InstaCortisol", which will make real-time and continuous measurements of personal exposure to psychosocial stress quantitatively, selectively, objectively, reliably, and securely by detecting Cortisol in interstitial fluid (ISF). ISF will be harvested using our proprietary minimally invasive device called "LightAccess". The proposed product involves the integration of several cutting edge technologies. The product would require virtually no action on the part of the subject or patient thereby greatly reducing error associated with improper use. These innovations would provide a significantly improved method for assessing a molecule that plays an important role in many biological processes and disease states.

描述（由申请人提供）：“StressSmart” 反复承受社会心理压力与多种人类疾病（例如癌症、毒瘾、心脏病、抑郁症）相关，这些疾病造成巨大的公共健康负担。然而，我们的进展受到限制，因为缺乏一种便携式设备，该设备可以快速、可靠、非侵入性和连续地实时测量个人在自然环境中所经历的社会心理压力。在这个跨学科项目中，我们建议开发一种名为 InstaCortisol(tm) 的新测试，通过测量受试者在自然环境中的皮质醇水平，可以可靠、选择性和快速地量化个人承受的压力。皮质醇是肾上腺皮质产生和分泌的主要类固醇，被用作压力的可靠指标。这种快速测试基于免疫电化学方法，当皮质醇夹在捕获物和检测抗体之间时，使用循环伏安法测量反应信号，检测抗体附着在碱性磷酸酶上。该项目有四个具体目标。首先是传感器制造：我们将在前两个月制造多个皮质醇传感器。其次是传感器评估：我们将测试传感器的灵敏度、特异性、酶/抗体稳定性、检测限、线性、准确性、动态范围、重复性、精密度、准确性、干扰、交叉反应性、漂移和传感器校准。我们预计会改变和/或优化制造方法，以达到每个类别的基准。第三是传感器性能：根据市售皮质醇传感器确定传感器性能。鉴于对现成皮质醇传感器的连续性能基准的需求，我们的目标是达到或超过。第四是设计集成可行性实验：皮质醇传感器芯片的虚拟评估及其集成到紧凑的易于使用的设计中。相关性：InstaCortisol(tm) 测试（正在第一阶段开发）将成为第二阶段 StressSmart(tm) 设备的一部分，使研究人员能够量化个人所经历的社会心理压力，就像个人在现实生活中所经历的那样。这种能力将增进我们对社会心理压力如何与造成巨大公共卫生负担的人类流行疾病的病因学中的遗传因素相互作用的理解。公共健康相关性：我们提议进行一种名为“InstaCortisol”的快速检测皮质醇测试，该测试将通过检测人体中的皮质醇，定量、选择性、客观、可靠和安全地实时、连续地测量个人承受的心理社会压力。间质液（ISF）。 ISF 将使用我们名为“LightAccess”的专有微创设备进行收获。拟议的产品涉及多项尖端技术的集成。该产品几乎不需要受试者或患者采取任何行动，从而大大减少与不当使用相关的错误。这些创新将为评估在许多生物过程和疾病状态中发挥重要作用的分子提供一种显着改进的方法。