NPC-16 Patchliner Octo (8 Channels)

NPC-16 Patchliner Octo（8 通道）

• 批准号: 8247584
• 负责人: Gail A Robertson
• 金额: $ 41.31万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-15 至 2013-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247584
• 关键词: Arrhythmia; Biological Assay; Cells; Collaborations; Collection; Communication; Data; Data Quality; Devices; Dialysis procedure; Drug effect disorder; Electrophysiology (science); Equipment; Funding; Grant; Hand; Inherited; Ion Channel; Manuals; Molecular; Nuclear Pore Complex; Perfusion; Physiological; Primary Cell Cultures; Productivity; Proteins; Research; Resistance; Safety; Technology; Temperature; Therapeutic; United States National Institutes of Health; Universities; Wisconsin; base; drug development; experience; extracellular; new technology; operation; patch clamp; research study

DESCRIPTION (provided by applicant): A significant bottleneck in ion channel research today is the need to use manual patch clamp recording to obtain high quality data. Research at the University of Wisconsin on safety assays for drug development, mechanisms of therapeutic drug action and the molecular basis for inherited and acquired arrhythmias would be significantly enhanced with the acquisition of new technology that dramatically increases experimental throughput. Whereas just a few data points can be reliably collected per day at the hands of a skilled manual patch technician, the Nanion Patchliner NPC-16 is an automated patch clamp device that allows the collection of up to 500 data points per day, largely unattended. Capabilities include gigaseal recordings with stable access resistance, temperature control to achieve physiologically-relevant conditions, perfusion of both intra- and extracellular compartments, compatibility with cultured cells, primary cells and bilayers containing purified proteins, and the option of using perforated patch to avoid dialysis of intracellular components. Many of these capabilities cannot be replicated using the manual patch (e.g., simultaneous application to extra- and intracellular compartments) or are extraordinarily difficult (patching at physiological temperatures). The users all have extensive experience with a wide range of electrophysiology approaches including patch clamp, as does the operating technician who will oversee daily operations. A management plan is developed allowing for online registration for equipment use and communication among primary users. It is expected the device will not only enhance the productivity toward grant aims of the five NIH-funded users, but will open opportunities for collaborations and for experiments not previously conceivable with traditional technologies.

描述（由申请人提供）：当今离子通道研究的一个重要瓶颈是需要使用手动膜片钳记录来获得高质量的数据。 威斯康星大学在药物开发安全测定、治疗药物作用机制以及遗传性和获得性心律失常的分子基础方面的研究将随着新技术的获得而显着增强，从而大大提高实验通量。 虽然熟练的手动贴片技术人员每天只能可靠地收集几个数据点，但 Nanion Patchliner NPC-16 是一种自动膜片钳设备，每天可以收集多达 500 个数据点，基本上无需有人值守。 功能包括具有稳定接入电阻的千兆密封记录、实现生理相关条件的温度控制、细胞内和细胞外区室的灌注、与培养细胞、原代细胞和含有纯化蛋白质的双层的兼容性，以及使用穿孔贴片以避免透析的选项细胞内成分。 其中许多功能无法使用手动补丁来复制（例如，同时应用于细胞外和细胞内区室）或者非常困难（在生理温度下进行补丁）。 用户都对包括膜片钳在内的各种电生理学方法拥有丰富的经验，监督日常操作的操作技术人员也是如此。 制定了一项管理计划，允许在线注册设备使用和主要用户之间的沟通。 预计该设备不仅将提高 NIH 资助的五个用户的资助目标的生产力，而且将为传统技术以前无法想象的合作和实验提供机会。