Laser Spinning Disc Confocal System for live-cell and live-organism microscopy

用于活细胞和活有机体显微镜的激光转盘共焦系统

• 批准号: 8243974
• 负责人: Martha C Soto
• 金额: $ 38.6万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8243974
• 关键词: Actins; Address; Aging; Animal Model; Beginning of Life; Biological; Biological Models; Biological Process; Caenorhabditis elegans; Cell physiology; Cells; Cellular biology; Cilia; Cytoskeleton; Data; Development; Disease; Drosophila genus; Embryo; Embryonic Development; Endocytosis; Event; Extracellular Matrix; Fertility; Fertilization; Funding; Germ Cells; Grant; Growth and Development function; Health; Heart; Homeostasis; Human; Image; Institution; Lasers; Life; Longevity; Manuscripts; Meiosis; Microscope; Microscopy; Morphogenesis; Natural regeneration; Nerve Degeneration; Neurons; New Jersey; Oocytes; Organism; Process; Proteins; Recovery; Regulation; Request for Applications; Research; Research Personnel; Research Project Grants; Resource Sharing; Role; Schools; System; Tissues; United States National Institutes of Health; Universities; Wood material; Work; axonal guidance; base; ciliopathy; data acquisition; egg; egg surface sperm receptor; improved; injured; instrument; interest; medical schools; migration; nervous system development; protein transport; receptor; trafficking

DESCRIPTION (provided by applicant): This application requests funds to purchase a new laser spinning disc microscope (LSDM) to be used as a shared resource for researchers at Rutgers University and Robert Wood Johnson Medical School (RWJMS), two schools on a shared campus in Piscataway, New Jersey. There is currently no laser spinning disc microscope (LSDM) available anywhere on the Rutgers or RWJMS campuses. The NIH-funded researchers requesting this instrument are studying fundamental problems in cell biology, taking advantage of model organisms to study conserved biological processes that are essential for healthy growth and development and that are compromised in disease conditions. C. elegans studies of longevity and neurodegeneration in the Driscoll lab are identifying the processes and the molecules that control lifespan and that become altered in injured neurons. The Wadsworth lab has identified conserved molecules required for proper axonal migrations across metazoan species. Studies of endocytosis in the Grant lab have identified key mechanisms for the regulation of receptor trafficking, identifying many new regulators in worms and humans. C. elegans studies of the regulation of the actin cytoskeleton by the Extracellular Matrix during embryonic development in the Soto Lab are identifying new roles for axonal guidance molecules organizing tissues, and identifying new components of the actin polarization machinery. The Barr Lab uses cilia in developing C. elegans as a model system to study human ciliopathies, or diseases of cilia. Drosophila studies of meiosis in the McKim lab have identified molecules required for proper fertility through the development of germ cells, while C. elegans studies in the Singson lab have identified the first sperm receptor and other molecules required for fertilization and the egg-to-oocyte transition. Live imaging of biological events is required to analyze these cellular processes, yet all of these researchers lack access to a local laser spinning disc confocal microscope. Having this instrument available would have an immediate impact: during a one-month demonstration of the Zeiss LSDM system three manuscripts incorporated images obtained from the demonstration instrument, and the instrument was in constant use. This instrument will clearly accelerate the acquisition of data by the 7 NIH-funded researchers listed in this proposal. The data obtained will dramatically enhance our understanding of such essential cellular phenomena as protein trafficking, polarization of the cytoskeleton and regeneration in neurons. PUBLIC HEALTH RELEVANCE: The addition of a laser spinning disc confocal microscopy system for the researchers at two adjacent universities on a shared campus, Rutgers University and Robert Wood Johnson Medical School, will allow these scientists to pursue research projects that will provide us with the insights necessary to understand how cells behave during normal development and in disease states. These researchers study essential processes of cells including how cells move, how cells like neurons are healed after injury, and how molecules are localized correctly to specific regions of cells.

描述（由申请人提供）：本申请要求资金购买新型激光旋转圆盘显微镜 (LSDM)，以用作罗格斯大学和罗伯特伍德约翰逊医学院 (RWJMS) 这两个共享校园的学校的研究人员的共享资源位于新泽西州皮斯卡塔韦。目前罗格斯大学或 RWJMS 校园内没有任何可用的激光旋转圆盘显微镜 (LSDM)。美国国立卫生研究院资助的研究人员需要该仪器来研究细胞生物学的基本问题，利用模式生物来研究对健康生长和发育至关重要以及在疾病条件下受到损害的保守生物过程。德里斯科尔实验室对线虫寿命和神经退行性变的研究正在确定控制寿命以及在受损神经元中发生改变的过程和分子。沃兹沃斯实验室已经确定了后生动物物种中轴突正确迁移所需的保守分子。格兰特实验室对内吞作用的研究已经确定了受体运输调节的关键机制，并确定了蠕虫和人类中的许多新调节因子。索托实验室在胚胎发育过程中对线虫细胞外基质对肌动蛋白细胞骨架的调节的研究正在确定轴突导向分子组织组织的新作用，并确定肌动蛋白极化机制的新组成部分。巴尔实验室在开发秀丽隐杆线虫时使用纤毛作为研究人类纤毛病或纤毛疾病的模型系统。 McKim 实验室的果蝇减数分裂研究已经确定了生殖细胞发育过程中正常生育所需的分子，而 Singson 实验室的线虫研究则确定了第一个精子受体以及受精和卵子到卵母细胞所需的其他分子过渡。分析这些细胞过程需要对生物事件进行实时成像，但所有这些研究人员都无法使用本地激光旋转圆盘共聚焦显微镜。拥有该仪器将产生立竿见影的效果：在蔡司 LSDM 系统为期一个月的演示中，三份手稿合并了从演示仪器获得的图像，并且该仪器一直在使用。该仪器显然将加速本提案中列出的 7 名 NIH 资助的研究人员获取数据的速度。获得的数据将极大地增强我们对蛋白质运输、细胞骨架极化和神经元再生等基本细胞现象的理解。 公共健康相关性：为位于同一校园的两所相邻大学（罗格斯大学和罗伯特伍德约翰逊医学院）的研究人员增加激光转盘共聚焦显微镜系统，将使这些科学家能够开展研究项目，为我们提供见解了解细胞在正常发育和疾病状态下的行为是必要的。这些研究人员研究细胞的基本过程，包括细胞如何移动、神经元等细胞如何在受伤后愈合，以及分子如何正确定位到细胞的特定区域。