Yellow Fever/Dengue Virus Competence in Aedes aegypt aegypti/formosus in Senegal

塞内加尔埃及伊蚊/台湾伊蚊的黄热病/登革热病毒能力

基本信息

批准号：
8291283
负责人：
WILLIAM C. BLACK
金额：
$ 35.76万
依托单位：
COLORADO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-15 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8291283
关键词：
Abdomen Address Adult Aedes Africa Africa South of the Sahara African Agreement Area Asians Behavior Burkina Faso Caribbean region Characteristics Chest Chromosome Mapping Chromosome inversion Chromosomes Chromosomes, Human, Pair 1 Chromosomes, Human, Pair 2 Chromosomes, Human, Pair 3 Collection Competence Complementary DNA Culicidae Data Dengue Dengue Virus Disease Funding Gambia Genes Genetic Genetic Markers Genetic Recombination Genetic Vectors Genome Genome Mappings Geographic Distribution Geographic Locations Ghana Goals Habitats Human Hybrids Individual Infection Island Isoenzymes Jamaica Knowledge Licensing Light Literature Location Maps Measures Midgut Mitochondria Mitochondrial DNA Mosquito Control Nuclear Paracentric Inversion Parents Pattern Penetration Poaceae Population Population Dynamics Population Genetics Predisposition Prevalence Publications Publishing Quantitative Trait Loci Relative (related person)Research Senegal Serotyping Single Nucleotide Polymorphism Site Solid South America Surveys Testing Time Vaccines Virus Visit Work Yellow Fever Yellow Fever Virus Infection Yellow fever virus base conditioning disorder control egg forest insight offspring research study trait vector vector control

项目摘要

Aedes aegypti sensu latu (s.l.) is the primary vector to humans of yellow fever virus (YFV) and the four serotypes of dengue virus (DENV1-4). Without a licensed vaccine for DENV, and despite the existence of a safe, effective YFV vaccine, control of these diseases still primarily relies on vector control. Our long term goal is to increase knowledge of local population dynamics to generate more focused, appropriate, and hopefully sustainable control strategies. There are two recognized subspecies of Ae. aegypti: the presumed ancestral form Ae. aegypti formosus (Aaf), a sylvan mosquito inhabiting forest habitats in sub-Saharan Africa; and Ae. aegypti aegypti (Aaa), found circumglobally in tropical and subtropical regions in close association with humans. We have been studying gene flow, vector competence and the distribution of Aaa and Aaf in Senegal. We present data that show a northwest-southeast cline in the abundance of the two subspecies. Northwest collections consist of only Aaa while collections in the southeast are all Aaf. The two subspecies were sympatric in six tall grass savanna and scrub sites in central Senegal. Consistent with the literature, competence for DENV2 is correlated with the distribution of the subspecies. Northwestern Aaa collections have a high disseminated infection rate (DIR) while southeast Aaf collections have a low DIR associated with a midgut escape barrier for DENV2. Curiously, our data and a recent publication suggest continuous gene flow across all geographic locations and subspecies in Senegal. This presents a paradox: How can the distribution of vector competence and subspecies be regional when gene flow is continuous? We present exciting data from a QTL mapping experiment gone wrong that demonstrate reduced recombination in Aaa/Aaf hybrids, consistent with an hypothesis that chromosomal inversions exist between the two subspecies. Inversions would allow for gene flow between subspecies while preserving associations in subspecies between morphological markers and vector competence. The possibility of inversions in Ae. aegypti s.l. has been discussed but never proven due to an inability to accurately resolve their polytene chromosomes. Our specific aims are to generate an intensive linkage map of Aaf to define presumptive inversions. Markers arising from this map will be used to define the geographic distribution of inversions in Senegal. We then propose to map Aaf QTL that condition susceptibility to DENV2 and YFV. We also plan to visit 50 additional sites in Senegal not covered in our initial survey to provide a more thorough coverage of vector competence and the prevalence of the subspecies.

埃及伊蚊 (Aedes aegypti sensu latu) (s.l.) 是人类黄热病病毒 (YFV) 的主要媒介，登革热病毒的四种血清型（DENV1-4）。没有获得许可的 DENV 疫苗，并且尽管存在安全、有效的 YFV 疫苗，但这些疾病的控制仍然主要依靠矢量控制。我们的长期目标是增加当地居民的知识动态产生更有针对性、适当且有望可持续的控制策略。伊蚊有两个公认的亚种。埃及伊蚊（aegypti）：推测的祖先形式 Ae。埃及伊蚊 formosus (Aaf)，一种栖息在撒哈拉以南非洲森林栖息地的森林蚊子；和艾。埃及伊蚊 (Aaa)，分布于全球热带和亚热带地区附近与人类的联系。我们一直在研究基因流、载体能力和 Aaa 和 Aaf 在塞内加尔的分布。我们提供的数据显示西北-东南斜线两个亚种的丰富程度。西北系列仅包含 Aaa，而东南部的藏品都是Aaf。这两个亚种共栖于六棵高草丛中塞内加尔中部的稀树草原和灌木丛地区。与文献一致，能力 DENV2与亚种的分布相关。西北 Aaa 收藏有传播性感染率 (DIR) 高，而 Aaf 东南部收集的 DIR 低与 DENV2 的中肠逃逸屏障有关。奇怪的是，我们的数据和最近的出版物表明，基因在所有地理位置和亚种之间持续流动塞内加尔。这就提出了一个悖论：矢量能力的分布和当基因流连续时亚种是区域性的吗？我们提供来自以下机构的令人兴奋的数据： QTL 作图实验出错，表明 Aaa/Aaf 重组减少杂种，与两者之间存在染色体倒位的假设一致亚种。倒置将允许亚种之间的基因流动，同时保留亚种形态标记与媒介能力之间的关联。这 Ae 中反转的可能性。埃及航空公司已经被讨论过但从未被证实，因为无法准确解析其多线染色体。我们的具体目标是产生 Aaf 的密集连锁图来定义假定的倒转。从此地图中产生的标记将用于定义塞内加尔逆温的地理分布。然后我们建议定位对 DENV2 和 YFV 易感性的 Aaf QTL。我们还计划参观 50 我们的初步调查未涵盖塞内加尔的其他地点，以提供更全面的覆盖范围媒介能力和亚种的流行程度。