PALM (PHOTOACTIVATED LOCALIZATION MICROSCOPY)

PALM（光激活定位显微镜）

• 批准号: 8365750
• 负责人: ENRICO GRATTON
• 金额: $ 5.62万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-20 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365750
• 关键词: Computer software; Fluorescence; Funding; Grant; Laboratories; Microscopy; National Center for Research Resources; Principal Investigator; Proteins; Research; Research Infrastructure; Resolution; Resources; Source; United States National Institutes of Health; Writing; cost; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. My project consists in setting up both the hardware and the software for doing PALM. I will need to combine the optimal wavelengths for the photoactivatable protein we have (tdEosFP), study the the most suitable combination of dichroic mirrors and filters in order to acquire most informations with the camera. Meanwhile I will write a program in Matlab that will allow me to localize the photoactivated molecules and reach the super-resolution.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 我的项目包括设置用于 PALM 的硬件和软件。我需要结合我们拥有的光激活蛋白（tdEosFP）的最佳波长，研究二向色镜和滤光片的最合适组合，以便用相机获取大部分信息。同时我将在 Matlab 中编写一个程序，使我能够定位光激活分子并达到超分辨率。