STRUCTURE FUNCTION RELATIONSHIPS IN POLYSACCHARIDES

多糖的结构功能关系

• 批准号: 7956846
• 负责人: SRINIVAS JANASWAMY
• 金额: $ 0.47万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956846
• 关键词: Biopolymers; Carrageenan; Complex; Computer Retrieval of Information on Scientific Projects Database; Drug Carriers; Fiber; Food; Funding; Glycosaminoglycans; Goals; Grant; Institution; Lateral; Methods; Molecular Structure; Pattern; Pharmaceutical Preparations; Pharmacologic Substance; Plants; Polysaccharides; Process; Research; Research Personnel; Resources; Solvents; Source; Specimen; Structure; Structure-Activity Relationship; System; Testing; United States National Institutes of Health; Wood material; analog; galactoglucomannan; gellan; glucuronoxylomannan; insight; lambda Carrageenan; small molecule; solute; structural biology; three dimensional structure; xanthan gum

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our long term goals are to elucidate biopolymers molecular structures, especially polysaccharides, and their interactions with solvent and solute molecules and the synergistic interactions in mixed polysaccharides towards gaining insights about their structure-function relationships. In this regard, the main thrust of this proposal is centered on determining the three-dimensional structures of a number of biologically important and industrially useful polysaccharides. Our ongoing study will include, but not limited to (as need arises), the specimens from algal (iota, kappa and lambda carrageenans); bacterial (gellan analog and cepacian); fungal (glucuronoxylomannan); plant (rhamnogalacturonan); wood (galactoglucomannan) and a binary system (bacterial xanthan:plant glucomannan and bacterial acetan:plant glucomannan). Further, we are in the process of exploring the use of polysaccharide fibers as possible drug carriers. In this regard, initially, carrageenan fibers complexed with some known small molecules will be tested towards obtaining crystalline diffraction patterns and delineating the drug-carrageenan interactions. These polysaccharides or polysaccharide complexes have an inherent tendency to form helical structures with only limited lateral ordering and are not amenable for growing single crystals. Hence, fiber diffraction is the only method of choice for visualizing their three-dimensional organization. Such structural information is essential towards understanding their interactions with solvent and solute molecules as well as with other polysaccharides for their effective utilization in food and pharmaceutical applications. The results will further highlight the conformational space available to polysaccharides as they occur in glycosaminoglycans and cellular walls.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们的长期目标是阐明生物聚合物分子结构，特别是多糖，及其与溶剂和溶质分子的相互作用以及混合多糖中的协同相互作用，以获得对其结构功能关系的见解。在这方面，该提案的主旨集中在确定许多具有生物学意义和工业用途的多糖的三维结构。我们正在进行的研究将包括但不限于（根据需要）藻类标本（iota、kappa 和 lambda 角叉菜胶）；细菌（结冷胶类似物和洋葱）；真菌（葡萄糖醛酸木甘露聚糖）；植物（鼠李半乳糖醛酸）；木材（半乳葡甘露聚糖）和二元系统（细菌黄原胶：植物葡甘聚糖和细菌乙酰：植物葡甘聚糖）。此外，我们正在探索使用多糖纤维作为可能的药物载体。在这方面，首先将测试与一些已知小分子复合的卡拉胶纤维，以获得晶体衍射图案并描绘药物-卡拉胶相互作用。 这些多糖或多糖复合物具有形成仅具有有限横向排序的螺旋结构的固有倾向，并且不适合生长单晶。因此，纤维衍射是可视化其三维组织的唯一选择方法。这些结构信息对于理解它们与溶剂和溶质分子以及与其他多糖的相互作用以使其在食品和制药应用中的有效利用至关重要。结果将进一步强调多糖在糖胺聚糖和细胞壁中存在时可用的构象空间。