DERIVING MOLECULAR MOTION FROM CRYOEM MAP

从 CryOEM 图推导出分子运动

• 批准号: 8361090
• 负责人: Lydia E. Kavraki
• 金额: $ 2.45万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361090
• 关键词: Behavior; Biochemical; Biological; Cryoelectron Microscopy; Electron Microscopy; Funding; Grant; Image; Individual; Maps; Molecular; Molecular Conformation; Motion; National Center for Research Resources; Principal Investigator; Process; Research; Research Infrastructure; Resources; Sampling; Series; Source; Structure; United States National Institutes of Health; cost; macromolecular assembly; macromolecule; reconstruction; Behavior; Biochemical; Biological; Cryoelectron Microscopy; Electron Microscopy; Funding; Grant; Image; Individual; Maps; Molecular; Molecular Conformation; Motion; National Center for Research Resources; Principal Investigator; Process; Research; Research Infrastructure; Resources; Sampling; Series; Source; Structure; United States National Institutes of Health; cost; macromolecular assembly; macromolecule; reconstruction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Perhaps the most outstanding contribution of cryoEM is the ability to image macromolecular assemblies under nearly native conditions. However, the drawback to this is that the sample is often a mixture of macromolecules in different conformations, reflecting the natural and intrinsic behavior of a biological machine. Computational and biochemical approaches have sought to isolate these individual conformations, allowing for the processing and eventual reconstruction of several structures in the different conformations. This will provide us with a unique opportunity to visualize the structural dynamics of these machines in a series of snapshots, but stops short of answering more fundamental questions about the motions associated with the different conformations.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 冷冻人最杰出的贡献也许是能够成像大分子 在几乎本地条件下的组装。但是，对此的缺点是样本 通常是不同构象中的大分子的混合物，反映了自然和 生物机器的内在行为。计算和生化方法具有 试图隔离这些个体构象，允许处理和最终 在不同构象中重建几个结构。这将为我们提供 独特的机会，可视化这些机器的结构动力学 快照，但没有回答有关动作的更多基本问题 与不同的构象相关。