3D IMAGING OF GAS EXCHANGE USING HYPERPOLARIZED 129XE MRI

使用超偏振 129XE MRI 对气体交换进行 3D 成像

• 批准号: 8363169
• 负责人: ZACKARY I CLEVELAND
• 金额: $ 1.84万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363169
• 关键词: Alveolar; Animal Model; Blood gas; Chemicals; Development; Diffuse; Erythrocytes; Fibrosis; Funding; Gases; Grant; Health; Image; Lung; Lung diseases; Magnetic Resonance Imaging; Methods; Microscopy; National Center for Research Resources; Phase; Principal Investigator; Pulmonary Gas Exchange; Research; Research Infrastructure; Resources; Solubility; Source; Staging; Thick; Three-Dimensional Imaging; Time; Tissues; United States National Institutes of Health; Work; Xenon; base; cost; in vivo; research study; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The primary aim is to develop a hyperpolarized (HP) 129Xe MRI-based method to study three dimensional pulmonary gas exchange in vivo. This method will exploit the large chemical shift range of 129Xe and the reasonably high solubility of xenon in tissues to differentially image the uptake of gas-phase, alveolar 129Xe by both the pulmonary blood/gas barrier tissue and red blood cells. Before reaching the red blood cells, HP 129Xe must first diffuse across the barrier tissue. Since diffusive transport times scale with the barrier thickness squared, 129Xe MR is capable of detecting barrier thickness changes as small as several microns. The initial stages of this work will focus on developing 3D gas exchange imaging capabilities. These efforts may involve constructing a gas exchange phantom that produces 129Xe NMR spectra that are similar to those observed in vivo before performing experiments with health controls. Following the development of 3D imaging capabilities, gas exchange in the healthy controls will be compared with well-known small animal models of pulmonary diseases involving gas exchange abnormalities such as fibrosis.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 主要目的是开发基于超极化（HP）129XE MRI的方法来研究体内三维肺气交换。 该方法将利用肺血/气屏组织和红细胞的巨大化学移位范围和组织在组织中的较高的化学移位范围和氙气溶解度相当高，以差异化气相的吸收，肺泡129x。在到达红细胞之前，HP 129XE必须首先在屏障组织中扩散。由于具有屏障厚度平方的扩散传输时间尺度，因此129倍MR能够检测到屏障厚度的变化至少几微米。这项工作的初始阶段将着重于开发3D气体交换成像功能。这些努力可能涉及构建一个产生129 XE NMR光谱的气体交换幻影，该光谱与体内观察到的光谱相似，然后进行健康控制实验。在发展3D成像功能之后，将在健康对照中的气体交换与涉及气体交换异常（如纤维化）的肺部疾病的众所周知的小动物模型进行比较。