Microarray & Genomics

微阵列

• 批准号: 8375980
• 负责人: Norma Jean Nowak
• 金额: $ 28.88万
• 依托单位: ROSWELL PARK CANCER INSTITUTE CORP
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8375980
• 关键词: Antibodies; Automation; Award; Bioinformatics; Biological Assay; Budgets; Cancer Center Support Grant; Color; Complementary DNA; Contracts; Custom; DNA; Data Analyses; Development; Faculty; Foundations; Funding; Gene Expression; Gene Frequency; Generations; Genes; Genome; Genomic Library; Genomics; Genotype; Grant; Human; Human Genome; Human Genome Project; Hybridization Array; Image; Image Analysis; Leadership; Libraries; Ligands; MALDI-TOF Mass Spectrometry; Maintenance; Methylation; Mus; Oligonucleotide Microarrays; Oligonucleotides; Peer Review; Preparation; Printing; Publications; RNA; Rattus; Recording of previous events; Research Infrastructure; Research Personnel; Resources; Robotics; Roswell Park Cancer Institute; SNP genotyping; Sampling; Scanning; Screening procedure; Services; Slide; Strategic Planning; Technology; Work; base; cDNA Library; cyanine dye 5; design; flexibility; high throughput technology; member; mouse genome; programs; technology development

The Microarray & Genomics Resource provides a full range of service in technologies for applied genomics. The importance of the Resource to the CCSG is reflected by a strong track record of utilization, publications and awarded grants and contracts. The Resource has a rich history of expertise and accomplishment in the Human Genome Project that laid the foundation for array technology development and analysis, development and implementation of high-throughput technologies and distribution of the RPCI BAC/PAC resources worldwide. The Microarray & Genomics Resource is composed of three complementary divisions: (1) The Genomics Division contributes to the application of human and mouse BAG clones for downstream functional analysis. It provides the infrastructure for automation through robotics for large-scale projects. Fifteen BAC/PAC genomic libraries and two cDNA libraries are available for screening, clone selection, characterization and distribution within RPCI. (2) The Microarray Division provides custom array design and analysis such as BAC-based array CGH for the human and mouse genomes, and gene expression, commercial platforms for two-color human, mouse and rat gene expression. Copy number studies are also available using Agilent and NimbleGen whole-genome oligonucleotide arrays. Investigators can request microarray services, including RNA/DNA isolation, amplification, Cy3 and Cy5 probe generation, hybridizations, custom-made cDNA, antibody, ligand and genomic arrays, scanning of microarrayed slides, image acquisition, normalization of data and analysis. (3) The Genotyping Division provides high-throughput genotyping of SNPs, SNP discovery and quantitative methylation services utilizing the Sequenom MALDI-TOF mass spectrometry platform. Investigators can request flexible assays designed for focused genotyping studies on large sample sets for as few as ten to as many as several hundred SNPs or genes. Over the project period, 54 CCSG Program members (43 with peer-reviewed funding) from five CCSG programs utilized the Resource. The Strategic Plan is to continue to provide access to state-of-theart technology facilitating large-scale and/or global analyses for applied genomics. The Director works closely with the CCSG leadership and members to guide, educate and support genomic endeavors. With the recruitment of new leadership and faculty in the CSBT, Til and MTET Programs, it is anticipated that CCSG members in all six CCSG programs will utilize this Resource. The Resource is used by five Programs and 46% of users are CCSG members. $121,671 in CCSG support is requested, representing 9% of the total operating budget.

微阵列和基因组学资源为应用的技术提供了全方位的服务 基因组学。资源对CCSG的重要性反映了利用的强烈记录， 出版物并授予赠款和合同。该资源具有丰富的专业历史和 人类基因组项目的成就，奠定了阵列技术开发基础的基础 以及高通量技术的分析，开发和实施以及RPCI的分布 BAC/PAC资源在全球。微阵列和基因组资源由三个 互补部门： （1）基因组学部门有助于人类和鼠标袋克隆的应用 下游功能分析。它提供了通过机器人技术自动化的基础架构 项目。 15个BAC/PAC基因组库和两个cDNA库可用于筛选，克隆 RPCI中的选择，表征和分布。 （2）微阵列部提供自定义阵列设计和分析，例如基于BAC的阵列 人类和小鼠基因组的CGH以及基因表达，两色人的商业平台， 小鼠和大鼠基因表达。还可以使用Agilent和Nimblegen进行拷贝数研究 全基因组寡核苷酸阵列。调查人员可以请求微阵列服务，包括RNA/DNA 分离，扩增，CY3和CY5探针产生，杂交，定制cDNA，抗体，配体 以及基因组阵列，微阵列幻灯片的扫描，图像采集，数据归一化和分析。 （3）基因分型分裂提供了SNP，SNP发现和 利用序列化MALDI-TOF质谱平台的定量甲基化服务。 调查人员可以要求为针对大型样本集的集中基因分型研究而设计的灵活测定法 只有多达十到几百个SNP或基因。 在整个项目期间，有54名CCSG计划成员（43位由同行评审的资金）五个 CCSG程序利用了资源。战略计划是继续提供最先进的访问权限 技术促进应用基因组学的大规模和/或全球分析。导演工作 与CCSG领导力和成员紧密联系，以指导，教育和支持基因组努力。和 在CSBT，TIL和MTET计划中招募新的领导力和教师，预计 CCSG成员在所有六个CCSG程序中都将利用此资源。 该资源由五个程序使用，46％的用户是CCSG成员。 CCSG $ 121,671 请求支持，占总运营预算的9％。