GENES CONTROLLING PUBERTY ONSET

控制青春期开始的基因

• 批准号: 8358199
• 负责人: Ei Terasawa-Grilley
• 金额: $ 11.57万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358199
• 关键词: Agonist; Animals; Behavioral; Biological Assay; Brain; Development; Disease; Estrogens; Female; Frequencies; Funding; Genes; Gonadotropin Hormone Releasing Hormone; Grant; KISS1R gene; Measures; Methods; Microdialysis; National Center for Research Resources; Ovarian; Peptides; Physiologic pulse; Play; Primates; Principal Investigator; Puberty; Research; Research Infrastructure; Resources; Role; Services; Source; Time; United States National Institutes of Health; Wisconsin; cost; kisspeptin; median eminence; nonhuman primate; prepuberty; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objective: To investigate genes controlling the timing of puberty. DESCRIPTION: Understanding the mechanism of puberty in the brain is important, as some diseases and behavioral disturbances occur in association with puberty. The aim of this study is to investigate the role of kisspeptins in the timing of puberty. PROGRESS: In this study the role of kisspeptin in the pubertal increase in GnRH release was examined. The results suggest that kisspeptin-54 release in the stalk-median eminence measured by a microdialysis method was pulsatile and mean kisspeptin-54 levels and pulse frequency, but not pulse amplitude, increased at the onset of puberty. Moreover, the pubertal increase in kisspeptin-54 release was independent from the pubertal changes in circulating ovarian estrogens, as the pubertal increase in kisspeptin-54 release was also observed in ovariectomized females. Finally, developmental changes in GPR54 sensitivity to KP were examined by assessing the GnRH response to a KP agonist and antagonist, KP-10 and peptide 234, respectively. Results that the rate of GnRH response to neither KP-10 nor peptide 234 was different between prepubertal and pubertal groups, indicate that GPR54 sensitivity does not undergo developmental changes. Collectively, these results are consistent with the hypothesis that kisspeptin plays a role in puberty. This research used WNPRC Animal Services and Assay Services. PUBLICATION: Terasawa, E., Kurian, J.R., Guerriero, K.A., Kenealy, B.P., Hutz, E.D., and Keen, K.L. Recent discoveries on the control of gonadotrophin-releasing hormone neuronesin nonhuman primates. J. Neuroendocrinol. 22:630-638, 2010. PMID: 20456608, PMCID: PMC2908205.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 目的：研究控制青春期时间的基因。 描述： 了解青春期大脑的机制很重要，因为某些疾病 行为障碍的发生与青春期有关。本研究的目的 目的是研究 Kisspeptins 在青春期时机中的作用。 进步： 在这项研究中，Kisspeptin 在青春期 GnRH 释放增加中的作用是 检查了。结果表明 Kisspeptin-54 在茎中位释放 通过微透析方法测量的隆起是脉动的并且平均 Kisspeptin-54 水平和脉冲频率，但不是脉冲幅度，在开始时增加 青春期。此外，青春期 Kisspeptin-54 释放的增加是独立的 来自青春期循环卵巢雌激素的变化，随着青春期的增加 在切除卵巢的女性中也观察到 Kisspeptin-54 的释放。最后， 通过评估 GPR54 对 KP 敏感性的发育变化 GnRH 分别对 KP 激动剂和拮抗剂 KP-10 和肽 234 做出反应。 结果表明，GnRH 对 KP-10 和肽 234 的反应率均为 青春期前组和青春期组之间存在差异，表明 GPR54 敏感性 不发生发育变化。总的来说，这些结果是一致的 假设 Kisspeptin 在青春期发挥作用。 本研究使用了 WNPRC 动物服务和化验服务。 发布： Terasawa, E.、Kurian, J.R.、Guerriero, K.A.、Kenealy, B.P.、Hutz, E.D. 和 Keen, K.L. 促性腺激素释放激素神经胶质蛋白控制的最新发现 非人类灵长类动物。 J.神经内分泌。 22:630-638，2010 年。PMID：20456608，PMCID： PMC2908205。