GENES CONTROLLING PUBERTY ONSET

控制青春期开始的基因

• 批准号: 8358199
• 负责人: Ei Terasawa-Grilley
• 金额: $ 11.57万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358199
• 关键词: Agonist; Animals; Behavioral; Biological Assay; Brain; Development; Disease; Estrogens; Female; Frequencies; Funding; Genes; Gonadotropin Hormone Releasing Hormone; Grant; KISS1R gene; Measures; Methods; Microdialysis; National Center for Research Resources; Ovarian; Peptides; Physiologic pulse; Play; Primates; Principal Investigator; Puberty; Research; Research Infrastructure; Resources; Role; Services; Source; Time; United States National Institutes of Health; Wisconsin; cost; kisspeptin; median eminence; nonhuman primate; prepuberty; response; Agonist; Animals; Behavioral; Biological Assay; Brain; Development; Disease; Estrogens; Female; Frequencies; Funding; Genes; Gonadotropin Hormone Releasing Hormone; Grant; KISS1R gene; Measures; Methods; Microdialysis; National Center for Research Resources; Ovarian; Peptides; Physiologic pulse; Play; Primates; Principal Investigator; Puberty; Research; Research Infrastructure; Resources; Role; Services; Source; Time; United States National Institutes of Health; Wisconsin; cost; kisspeptin; median eminence; nonhuman primate; prepuberty; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objective: To investigate genes controlling the timing of puberty. DESCRIPTION: Understanding the mechanism of puberty in the brain is important, as some diseases and behavioral disturbances occur in association with puberty. The aim of this study is to investigate the role of kisspeptins in the timing of puberty. PROGRESS: In this study the role of kisspeptin in the pubertal increase in GnRH release was examined. The results suggest that kisspeptin-54 release in the stalk-median eminence measured by a microdialysis method was pulsatile and mean kisspeptin-54 levels and pulse frequency, but not pulse amplitude, increased at the onset of puberty. Moreover, the pubertal increase in kisspeptin-54 release was independent from the pubertal changes in circulating ovarian estrogens, as the pubertal increase in kisspeptin-54 release was also observed in ovariectomized females. Finally, developmental changes in GPR54 sensitivity to KP were examined by assessing the GnRH response to a KP agonist and antagonist, KP-10 and peptide 234, respectively. Results that the rate of GnRH response to neither KP-10 nor peptide 234 was different between prepubertal and pubertal groups, indicate that GPR54 sensitivity does not undergo developmental changes. Collectively, these results are consistent with the hypothesis that kisspeptin plays a role in puberty. This research used WNPRC Animal Services and Assay Services. PUBLICATION: Terasawa, E., Kurian, J.R., Guerriero, K.A., Kenealy, B.P., Hutz, E.D., and Keen, K.L. Recent discoveries on the control of gonadotrophin-releasing hormone neuronesin nonhuman primates. J. Neuroendocrinol. 22:630-638, 2010. PMID: 20456608, PMCID: PMC2908205.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 目的：研究控制青春期时间的基因。 描述： 了解大脑中青春期的机制很重要，因为某些疾病 与青春期有关的行为障碍发生。这项研究的目的 是为了研究亲肽蛋白在青春期的时机中的作用。 进步： 在这项研究中 检查。结果表明Kisspeptin-54在Stalk-Median中释放 通过微透析法测得的杰出性是脉动和平均亲肽素-54 水平和脉搏频率，但不脉冲幅度，在开始时增加 青春期。此外，Kisspeptin-54发行的青春期增长是独立的 随着青春期的增长，循环卵巢雌激素的青春期变化 在卵巢切除的女性中也观察到了亲肽素54的释放。最后， 通过评估了GPR54对KP的敏感性的发展变化 GnRH对KP激动剂和拮抗剂KP-10和肽234的反应分别。 结果表明，GNRH对KP-10和肽234的响应速率均不是 青春期前和青春期组之间的不同，表明GPR54敏感性 不经历发展变化。总的来说，这些结果是一致的 假设亲吻肽在青春期中起作用。 这项研究使用了WNPRC动物服务和测定服务。 发布： 特拉索（E. 关于控制促性腺营养蛋白释放激素神经元的最新发现 非人类灵长类动物。 J. Neuroenodocrinol。 22：630-638，2010。PMID：20456608，PMCID： PMC2908205。