Canine Histocompatibility Typing

犬组织相容性分型

基本信息

批准号：
8240008
负责人：
SCOTT Stoll GRAVES
金额：
$ 11.75万
依托单位：
FRED HUTCHINSON CANCER RESEARCH CENTER
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-03-08 至 2014-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8240008
关键词：
Alleles Allogenic Allografting Amino Acids Antigens Bacteriophages Base Sequence Breeding Canis familiaris Cell Line Cell Transplantation Cell membrane Chimerism Chromosomes Clinical Complementary DNA Dendritic Cells Exons Frequencies Gene Structure Genes Genome Genomics Genotype HLA Antigens Hematopoietic Histocompatibility Antigens Class II Histocompatibility Testing Human Human Resources Immunoglobulin Variable Region In Vitro Introns K562 Cells Knowledge Libraries Location MHC Class I Genes MHC Class II Genes Major Histocompatibility Complex Mammals Methods Minor Modeling Molecular Molecular Analysis Molecular Probes Molecular Structure Mus Names Nucleotides Organ Transplantation Outcome Pre-Clinical Model Principal Investigator Program Research Project Grants Research Project Grants Restriction fragment length polymorphism Role Scientist Serological Services Solid Stem cells T-Lymphocyte Techniques United States National Institutes of Health Variant base design genome database human leukocyte antigen gene meetings programs prospective web site

项目摘要

CORE B: CANINE HISTOCOMPATIBILITY TYPING The Canine Histocompatibility Typing Core is designed to meet the needs of the two canine research Projects 1 and 2 on this Program Project Grant. The Core personnel consist of a Core Director, a Staff Scientist, and one technician. The primary role of the service consists of canine histocompatibility typing to identify Dog Leukocyte Antigen (DLA)-identical littermate pairs for hematopoietic cell transplantation and to select pairs of dogs with desired histocompatibility type for breeding. These efforts are critical in order for the dog to function as a translational model for human hematopoietic cell transplantation. While the typing effort is focused on the two most polymorphic class I and class II loci, DLA-88 and DRB1, additional efforts will involve developing methods for typing 3 additional polyallelic DLAclass I loci. This Core will also generate dendritic cells, develop genetically modified K562 cell lines for expression of selected DLA class I alleles, and develop tetramers for minor antigen-specific T cell identification and characterization.

核心B：犬组织兼容性打字犬类组织相容性打字核心旨在满足两个犬研究的需求该计划项目赠款的项目1和2。核心人员由核心主任，员工组成科学家和一名技术员。服务的主要作用是犬类组织相容性打字到识别狗白细胞抗原（DLA） - 相同的同窝仔对造血细胞移植和至选择具有所需组织相容性类型的狗对繁殖。这些努力至关重要该狗充当人类造血细胞移植的转化模型。在打字时努力集中在两个最多态的I类和II类位点，DLA-88和DRB1，额外的努力将涉及开发用于键入3个附加polyallic dlaclass I基因座的方法。这个核心也将产生树突状细胞，开发出遗传修饰的K562细胞系，以表达选定的DLA类I类等位基因，并开发用于次要抗原特异性T细胞鉴定和表征的四聚体。