Novel nano-mechanical platform to investigate therapeutic sub-cellular mechanical

研究治疗性亚细胞力学的新型纳米力学平台

• 批准号: 8386103
• 负责人: Rupak M Rajachar
• 金额: $ 7.47万
• 依托单位: MICHIGAN TECHNOLOGICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8386103
• 关键词: Address; Adhesions; Affect; Area; Behavior; Biocompatible Materials; Biological; Cell Adhesion; Cells; Chemicals; Coupled; Deterioration; Development; Devices; Environment; Exploratory/Developmental Grant; Fibroblasts; Film; Frequencies; Gene Expression; Goals; Immune response; Implant; In Situ; In Vitro; Investigation; Lead; Mechanics; Modeling; Monitor; Morphology; Myofibroblast; Play; Property; Real-Time Systems; Research Project Grants; Role; Site; Stimulus; Surface; Surface Properties; System; Systems Development; Technology; Testing; Therapeutic; Therapeutic Effect; Time; Tissues; Ultrasonography; United States National Institutes of Health; base; cell behavior; cell type; clinical application; implantable device; implantation; improved; in vivo; interest; macrophage; nano; neutrophil; novel; novel strategies; research study; response; seal; submicron; surface coating; tool; vibration

DESCRIPTION (provided by applicant): This project seeks support for the development of a novel mechanically responsive material system to study the relationship between local cell-level mechanical perturbation and cell response to chemical-physical biomaterial surface properties. The coating is based on a magnetoelastic material that can be remotely set to vibrate at a predetermined amplitude and profile. We hypothesize that local sub-cellular mechanical stimulus can be used to optimize substrate-cellular interactions to improve the long-term stability at the tissue-implant interface. Ultimately we hope to use these coatings as a real-time system for modulation and monitoring the surface of implantable biomaterials. Preliminary experiments have indicated that submicron localized vibrations, such as those generated by the proposed vibration coating, could potentially be coupled with tailored biomaterial surface properties to selectively control cellular adhesion and gene expression to promote and maintain proper integration at the implant-tissue interface. The specific goal of this proposal is to characterize the relationship between small local vibrations, substrate material surface character, and concomitant cellular response to applied vibrations. PUBLIC HEALTH RELEVANCE: To date, there are no effective technologies for controlling the long-term deterioration of the biomaterial-tissue interface in implantable devices. Although this i an area of intense study with many novel approaches involving coatings and surface treatments, these approaches do not allow for in situ modulation of therapeutic effects. By developing the technology outlined in this proposal, it may be possible to monitor implant behavior as well as affect changes in the local mechanical and chemical environment. In addition, the results of this proposal will further impact the understanding of local vibrations asa therapeutic tool. Only recently has the mechanism of larger amplitude and frequency vibration (ultrasound) therapeutic effects on cell behavior become clearer. Our focal site-specific approach may lead to new strategies and applications for the use of these platform coatings in implantable biomaterials.

描述（由申请人提供）：该项目寻求支持开发一种新型机械响应材料系统，以研究局部细胞级机械扰动与细胞对化学物理生物材料表面特性的响应之间的关系。该涂层基于磁致弹性材料，可以远程设置以预定的幅度和轮廓振动。我们假设局部亚细胞机械刺激可用于优化基质-细胞相互作用，以提高长期稳定性 在组织-植入物界面处。最终我们希望将这些涂层用作实时系统来调节和监测可植入生物材料的表面。初步实验表明，亚微米局部振动（例如由所提出的振动涂层产生的振动）可能与定制的生物材料表面特性相结合，以选择性地控制细胞粘附和基因表达，从而促进和维持植入物-组织界面处的适当整合。该提案的具体目标是表征微小的局部振动、基底材料表面特征以及伴随的细胞对施加振动的响应之间的关系。 公共健康相关性：迄今为止，还没有有效的技术来控制植入式设备中生物材料-组织界面的长期恶化。尽管这是一个深入研究的领域，有许多涉及涂层和表面处理的新颖方法，但这些方法不允许对治疗效果进行原位调节。通过开发本提案中概述的技术，可以监测植入物行为并影响局部机械和化学环境的变化。此外，该提案的结果将进一步影响对局部振动作为治疗工具的理解。直到最近，较大振幅和频率的振动（超声波）对细胞行为的治疗作用的机制才变得更加清晰。我们的焦点特定位置方法可能会导致在可植入生物材料中使用这些平台涂层的新策略和应用。