Tissue Culture Core Facility

组织培养核心设施

• 批准号: 8376351
• 负责人: Steve Oglesbee
• 金额: $ 20.36万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8376351
• 关键词: Accounting; Area; B lymphocyte immortalization; B-Lymphocytes; Baculovirus Expression System; Basic Science; Bioreactors; Cancer Center; Cancer Center Support Grant; Cell Culture Techniques; Cell Line; Cells; Cellular biology; Clinical; Clinical Research; Collaborations; Complex; Consultations; Core Facility; Cost Savings; Country; Cryopreservation; Dedications; Development; Endotoxins; Fiber; Fingerprint; Funding; Future; Goals; Grant; Growth; Human; In Vitro; Karyotype determination procedure; Licensing; Membrane; Methods; Monoclonal Antibodies; Mycoplasma; Pathology; Peer Review; Procedures; Production; Quality Control; Reagent; Research; Research Personnel; Research Project Grants; Research Support; Resource Sharing; Serum Proteins; Services; Site; Supervision; Suspension substance; Suspensions; Techniques; Testing; Tissue Microarray; Tissues; Training; UNC Lineberger Comprehensive Cancer Center; cancer cell; cell bank; cell transformation; cell type; cost; design; discount; experience; high standard; innovation; member; monoclonal antibody production; operation; programs; research study; response; sterility testing; tissue culture

Tissue Culture Core Facility The goal of the this core is to provide UNC Lineberger Comprehensive Cancer Center members with expert services and quality controlled and innovative materials required for basic and clinical research involving in vitro cell culture and related testing services. This core provides services and support not found elsewhere at UNC. The facility has two main "divisions." The first is Cell Culture, with all of its services, products, and testing. Services are designed and implemented to cover a wide range of user needs, such as large scale cell and metabolite production from cell propagation, monoclonal antibody (Mab) production using standard procedures, and production of gram quantities of Mab using hollow fiber, bioreactor and membrane designed units. Many ofthe Mabs produced are serum- and protein-free and can be used without further concentration or purification. Cell cryopreservation services are available using a programmable rate freezer. Testing for sterility, endotoxin, and mycoplasma are routinely provided. The other "division" of the core focuses on clinical support through its immortalization of human B-cells using EBV. This area is being increased to Include cell line identification by fingerprinting and karyotyping where required. The facility is led by Stephen Oglesbee, Director, with over 34 years of direct experience. The Core adds value to the Center by providing a range of specialized services, centralized functions, and cost savings and convenience. Highlights of research supported by the core include: high human B-cell transformation rates (over 98%) and the development and testing of new specialized media either already licensed or soon to be managed for the good ofthe UNC LCCC. There are currently >900 cell lines kept on site. Peer-reviewed members (159) account for 80% of the total facility use. In 2009,172 cancer center members (all but 13 are peer -reviewed) from all programs used the core. Future plans include expansion of its human B-cell immortalization program in support of numerous projects with other methods of immortalization of other cell types. Cell line fingerprinting is becoming a newly added service. CCSG funds of $66,895 are requested, representing 5% of total operating costs.

组织培养核心设施 该核心的目的是为UNC Lineberger综合癌症中心成员提供 基础和临床研究所需的专家服务和质量控制和创新材料 涉及体外细胞培养和相关测试服务。该核心提供服务和支持找不到 在UNC的其他地方。 该设施有两个主要的“分区”。首先是细胞培养，并进行所有服务，产品和测试。 服务的设计和实施是为了满足广泛的用户需求，例如大型单元和 使用标准的细胞传播，单克隆抗体（MAB）产生的代谢产物产生 使用空心纤维，生物反应器和设计 单位。生产的许多单元不含血清和无蛋白质，并且可以不进一步使用 浓度或纯化。使用可编程速度可获得细胞冷冻保存服务 冰箱。常规提供对无菌，内毒素和支原体的测试。 核心的另一个“分裂”重点是通过其永生化人类B细胞的临床支持 使用EBV。该区域正在增加，包括通过指纹和核分型来识别细胞系。 需要的地方。 该设施由董事Stephen Oglesbee领导，拥有超过34年的直接经验。核心添加 通过提供一系列专业服务，集中职能和节省成本以及 方便。核心支持的研究的亮点包括：高人类B细胞转换率 （超过98％）以及已经获得许可或即将成为的新专业媒体的开发和测试 管理UNC LCCC的利益。目前，现场保留了> 900个单元线。同行评审 成员（159）占设施总使用的80％。在2009年，172名癌症中心成员（13岁以外的所有人都是 所有程序都使用核心。未来的计划包括扩展其人类B细胞 永生化计划支持许多其他细胞的永生方法 类型。细胞系指纹正在成为新添加的服务。要求CCSG资金为66,895美元， 代表总运营成本的5％。