Ceramic Electron Microscopy Grids for Cell Culturing and Multiscale Imaging

用于细胞培养和多尺度成像的陶瓷电子显微镜网格

• 批准号: 8326089
• 负责人: Oleg G. Polyakov
• 金额: $ 39.45万
• 依托单位: SYNKERA TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8326089
• 关键词: Address; Architecture; Automation; Biological; Biological Sciences; Biophysics; Case Study; Cell Culture Techniques; Cells; Cellular Structures; Cellular biology; Ceramics; Colorado; Communities; Cryoelectron Microscopy; Development; Electron Microscopy; Evaluation; Film; Freezing; Generations; Generic Drugs; Goals; Image; Imaging technology; Joints; Laboratories; Mainstreaming; Methods; Microscopy; National Institute of General Medical Sciences; Optics; Performance; Phase; Preparation; Process; Protocols documentation; Publications; Research; Research Institute; Research Personnel; Sampling; Specimen; Structure; Techniques; Technology; Time; Universities; base; bioimaging; cellular imaging; comparative; cost; design; electron tomography; imaging modality; improved; light microscopy; meetings; molecular imaging; molecular scale; novel; prototype; scale up; single molecule; success; three-dimensional modeling; Address; Architecture; Automation; Biological; Biological Sciences; Biophysics; Case Study; Cell Culture Techniques; Cells; Cellular Structures; Cellular biology; Ceramics; Colorado; Communities; Cryoelectron Microscopy; Development; Electron Microscopy; Evaluation; Film; Freezing; Generations; Generic Drugs; Goals; Image; Imaging technology; Joints; Laboratories; Mainstreaming; Methods; Microscopy; National Institute of General Medical Sciences; Optics; Performance; Phase; Preparation; Process; Protocols documentation; Publications; Research; Research Institute; Research Personnel; Sampling; Specimen; Structure; Techniques; Technology; Time; Universities; base; bioimaging; cellular imaging; comparative; cost; design; electron tomography; imaging modality; improved; light microscopy; meetings; molecular imaging; molecular scale; novel; prototype; scale up; single molecule; success; three-dimensional modeling

DESCRIPTION (provided by applicant): Improved supporting technologies for imaging of molecular and supramolecular structures within cells are needed to facilitate cell biology research, and are sought by the National Institute of General Medical Sciences (specifically, its Division of Cell Biology and Biophysics). Multiscale imaging, using cryo-electron tomography (cryo-ET) on supramolecular structures and single molecules, has proven in recent years to be a unique and invaluable method for highthroughput characterization of the dynamic 3D architecture of cells. Electron microscopy (EM) grids, used as substrates for supporting the biological and biomolecular specimens being imaged, are a critical component associated with this imaging method. New EM grid technology that decreases sample preparation cost and time, improves sample generation from culturing to freezing for cryo-ET, and increases imaging quality will allow researchers to more efficiently explore cellular architecture, at higher throughput. Synkera proposes a novel class of ceramic EM grids that feature an integrated thin support film that is highly compatible with cell culturing, light microscopy and cryo-ET. The grids will facilitate high-throughput, multiscale imaging of sub-cellular architecture and offer key advantages over state-of-the art products. The grids are also expected to be a competitive alternative in many other EM and culturing applications a 6-month Phase I project successfully demonstrated feasibility of the proposed grids architecture, as well as their potential in culturing, cryo-ET and multiscale imaging. Phase II aims to build on this success by further developing fabrication processes and ceramic EM grids designs, to fully realize the potential for multiscale imaging in functional prototypes. At least four academic partners will aide in this development process. The ultimate goal of the proposed project is a complete line of ceramic EM grids for a broad range of EM applications, from bioimaging to materials characterization.

描述（由申请人提供）：需要改进细胞内分子和超分子结构成像的支持技术，以促进细胞生物学研究，并由国家一般医学科学研究所（特别是其细胞生物学和生物物理学的划分）寻求。 在超分子结构和单分子上使用冷冻电子层析成像（Cryo-ET）的多尺度成像已证明是对细胞动态3D体系结构的高截止表征的独特而宝贵的方法。电子显微镜（EM）网格，用作支撑正在成像的生物学和生物分子标本的底物，是与这种成像方法相关的关键成分。降低样品制备成本和时间的新EM网格技术，改善了从培养到冷冻-ET培养的样本生成，并提高成像质量将使研究人员在更高的吞吐量下更有效地探索细胞体系结构。 Synkera提出了一类新型的陶瓷EM网格，其中具有与细胞培养，光学显微镜和冷冻-ET高度兼容的综合薄载膜。网格将促进高通量，多尺度的亚细胞体系结构，并提供与最先进的产品相比的关键优势。在许多其他EM和培养应用中，也有竞争性的替代方法，该网格成功地证明了拟议的网格体系结构的可行性6个月，以及它们在培养，冷冻-ET和多尺度成像方面的潜力。第二阶段的目的是通过进一步开发制造过程和陶瓷电网设计来建立这一成功，以充分实现功能原型中多尺度成像的潜力。至少有四个学术合作伙伴将有助于此开发过程。拟议项目的最终目标是陶瓷EM网格的完整线，用于广泛的EM应用，从生物成像到材料表征。