Effects of Reversible Lesions on Resting fMRI in Awake Macaques

可逆性损伤对清醒猕猴静息功能磁共振成像的影响

• 批准号: 8397754
• 负责人: ERIC F MOOSHAGIAN
• 金额: $ 5.54万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8397754
• 关键词: Affect; Agonist; Animals; Architecture; Area; Arousal; Attention; Basal Ganglia; Bilateral; Brain; Brain imaging; Brain region; Cell Nucleus; Cerebral cortex; Cognition; Cognitive; Conscious; Deep Brain Stimulation; Disease; Dorsal; Functional Imaging; Functional Magnetic Resonance Imaging; Graph; Human; Lesion; Light; Link; Macaca; Maintenance; Monitor; Monkeys; Muscimol; Neurons; Pattern; Play; Positioning Attribute; Pulvinar structure; Rehabilitation therapy; Research; Rest; Role; Sensory; Signal Transduction; Site; Structure; Thalamic Nuclei; Thalamic structure; Tissues; Traumatic Brain Injury; Work; alertness; awake; base; improved; insight; interest; nonhuman primate; novel; prospective; relating to nervous system; research study

DESCRIPTION (provided by applicant): This project examines a new but poorly understood finding from functional magnetic resonance imaging (fMRI) that is providing new insights into fundamental brain architecture. Functional imaging studies show that similar sets of non-contiguous brain regions are co-activated across a wide range of tasks. Remarkably, many of these co-activations are recapitulated when subjects are at rest. Thus, resting state fMRI (rs-fMRI) is thought to reflect task-related functional networks. It is of great interest to know what principles are responsible for the maintenance of these intrinsic co-activations. Most functional connectivity studies have focused on the cortex. Deep brain structures, including the thalamus, have been implicated in these networks, but little is known regarding exactly what role they might play. Most input to the cerebral cortex passes through the thalamus, including many sensory signals, cerebelar and basal ganglia inputs. As a result, the thalamus is in an ideal anatomical position to regulate or maintain functional connectivity. Focal lesions can reveal much about the structure and maintenance of functional connectivity. Progress in this area has focused on naturally occurring lesions. Experimental lesions in non-human primates can be precisely placed, and repeated to obtain statistical confidence in their effects. Recently, rs-fMRI in non-human primates has revealed cortical networks similar to those observed in humans. Muscimol, a GABAA agonist, can be injected into tissue to inhibit local activity, and the sites of inactivation can be precisely monitored. We propose to use reversible inactivation in the macaque monkey to examine the roles of the thalamus and cortex in maintaining resting state network functional connectivity, and to characterize the effects of inactivation on the networks using novel graph theoretic approaches. This work will shed light on the neuronal underpinnings of cortical connectivity and more generally, may inform rehabilitation approaches for traumatic brain injury and other disorders. PUBLIC HEALTH RELEVANCE: The thalamus and its connections are critical for cognition. Reduced cognitive capacity due to traumatic brain injury is sometimes improved by deep brain stimulation of the thalamus. This work will elucidate the neuronal underpinnings of cortical connectivity underlying normal cognition and may inform rehabilitation approaches for traumatic brain injury.

描述（由申请人提供）：该项目研究了功能磁共振成像（fMRI）中一项新的但人们知之甚少的发现，该发现为基本大脑结构提供了新的见解。功能成像研究表明，相似的非连续大脑区域在各种任务中被共同激活。值得注意的是，当受试者休息时，许多这些共同激活都会重现。因此，静息态功能磁共振成像（rs-fMRI）被认为反映了与任务相关的功能网络。了解哪些原理负责维持这些内在的共同激活是非常有趣的。大多数功能连接研究都集中在皮质上。包括丘脑在内的深层大脑结构与这些网络有关，但人们对它们到底扮演什么角色知之甚少。大脑皮层的大部分输入都通过丘脑，包括许多感觉信号、小脑和基底神经节的输入。因此，丘脑处于调节或维持功能连接的理想解剖位置。 局灶性病变可以揭示很多有关功能连接的结构和维持的信息。该领域的进展主要集中在自然发生的病变上。非人类灵长类动物的实验损伤可以精确放置并重复，以获得其效果的统计可信度。最近，rs-fMRI 在非人类灵长类动物中发现的皮质网络与在人类中观察到的相似。蝇蕈醇是一种 GABAA 激动剂，可以注射到组织中抑制局部活性，并且可以精确监测失活部位。我们建议在猕猴中使用可逆失活来检查丘脑和皮层在维持静息状态网络功能连接中的作用，并使用新颖的图论方法来表征失活对网络的影响。这项工作将揭示皮质连接的神经元基础，更广泛地说，可能会为创伤性脑损伤和其他疾病的康复方法提供信息。 公共卫生相关性：丘脑及其连接对于认知至关重要。脑外伤导致的认知能力下降有时可以通过丘脑的深部脑刺激得到改善。这项工作将阐明正常认知的皮层连接的神经元基础，并可能为创伤性脑损伤的康复方法提供信息。