Effects of Reversible Lesions on Resting fMRI in Awake Macaques

可逆性损伤对清醒猕猴静息功能磁共振成像的影响

• 批准号: 8397754
• 负责人: ERIC F MOOSHAGIAN
• 金额: $ 5.54万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8397754
• 关键词: Affect; Agonist; Animals; Architecture; Area; Arousal; Attention; Basal Ganglia; Bilateral; Brain; Brain imaging; Brain region; Cell Nucleus; Cerebral cortex; Cognition; Cognitive; Conscious; Deep Brain Stimulation; Disease; Dorsal; Functional Imaging; Functional Magnetic Resonance Imaging; Graph; Human; Lesion; Light; Link; Macaca; Maintenance; Monitor; Monkeys; Muscimol; Neurons; Pattern; Play; Positioning Attribute; Pulvinar structure; Rehabilitation therapy; Research; Rest; Role; Sensory; Signal Transduction; Site; Structure; Thalamic Nuclei; Thalamic structure; Tissues; Traumatic Brain Injury; Work; alertness; awake; base; improved; insight; interest; nonhuman primate; novel; prospective; relating to nervous system; research study

DESCRIPTION (provided by applicant): This project examines a new but poorly understood finding from functional magnetic resonance imaging (fMRI) that is providing new insights into fundamental brain architecture. Functional imaging studies show that similar sets of non-contiguous brain regions are co-activated across a wide range of tasks. Remarkably, many of these co-activations are recapitulated when subjects are at rest. Thus, resting state fMRI (rs-fMRI) is thought to reflect task-related functional networks. It is of great interest to know what principles are responsible for the maintenance of these intrinsic co-activations. Most functional connectivity studies have focused on the cortex. Deep brain structures, including the thalamus, have been implicated in these networks, but little is known regarding exactly what role they might play. Most input to the cerebral cortex passes through the thalamus, including many sensory signals, cerebelar and basal ganglia inputs. As a result, the thalamus is in an ideal anatomical position to regulate or maintain functional connectivity. Focal lesions can reveal much about the structure and maintenance of functional connectivity. Progress in this area has focused on naturally occurring lesions. Experimental lesions in non-human primates can be precisely placed, and repeated to obtain statistical confidence in their effects. Recently, rs-fMRI in non-human primates has revealed cortical networks similar to those observed in humans. Muscimol, a GABAA agonist, can be injected into tissue to inhibit local activity, and the sites of inactivation can be precisely monitored. We propose to use reversible inactivation in the macaque monkey to examine the roles of the thalamus and cortex in maintaining resting state network functional connectivity, and to characterize the effects of inactivation on the networks using novel graph theoretic approaches. This work will shed light on the neuronal underpinnings of cortical connectivity and more generally, may inform rehabilitation approaches for traumatic brain injury and other disorders. PUBLIC HEALTH RELEVANCE: The thalamus and its connections are critical for cognition. Reduced cognitive capacity due to traumatic brain injury is sometimes improved by deep brain stimulation of the thalamus. This work will elucidate the neuronal underpinnings of cortical connectivity underlying normal cognition and may inform rehabilitation approaches for traumatic brain injury.

描述（由申请人提供）：该项目从功能磁共振成像（fMRI）中研究了一个新的但知之甚少的发现，该发现正在为基本的大脑结构提供新的见解。功能成像研究表明，在广泛的任务中共同激活类似的非连续大脑区域。值得注意的是，当受试者处于静止状态时，其中许多共同激活会概括。因此，静止状态fMRI（RS-FMRI）被认为反映了与任务相关的功能网络。了解哪些原则是为了维持这些内在共同激活的原因是非常有趣的。大多数功能连通性研究都集中在皮层上。包括丘脑在内的深层大脑结构已经与这些网络有关，但是关于它们可能扮演的角色的确切作用知之甚少。大脑对大脑皮层的输入通过丘脑，包括许多感觉信号，小脑和基底神经节输入。结果，丘脑处于调节或维持功能连通性的理想解剖位置。 焦点病变可以揭示功能连接性的结构和维护。该领域的进展集中在自然发生的病变上。可以精确地放置非人类灵长类动物的实验病变，并重复以对其作用获得统计信心。最近，RS-FMRI 在非人类灵长类动物中，揭示了类似于人类观察到的皮质网络。 Muscimol是一种GABAA激动剂，可以注入组织中以抑制局部活性，并且可以精确监测失活的部位。我们建议在猕猴中使用可逆的失活，以检查丘脑和皮层在维持静止状态网络功能连接性中的作用，并使用新颖的图理论方法来表征失活对网络的影响。这项工作将阐明皮质连通性的神经元基础，更普遍地可能为康复方法提供了脑损伤和其他疾病的康复方法。 公共卫生相关性：丘脑及其联系对于认知至关重要。由于丘脑的深脑刺激，有时会改善由于脑部损伤而导致的认知能力。这项工作将阐明正常认知基础的皮质连通性的神经元基础，并可能为创伤性脑损伤提供康复方法。