Core--Viral, Cellular and Molecular Biology Facility

核心——病毒、细胞和分子生物学设施

• 批准号: 7456544
• 负责人: Ronald G Collman
• 金额: $ 31.68万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7456544
• 关键词: Acquired Immunodeficiency Syndrome; Biological; Biological Assay; CCR5 gene; Cells; Collaborations; Communities; Computer software; Containment; Core Facility; Custom; Data Analyses; Development; Ensure; Enzyme-Linked Immunosorbent Assay; Floor; Genotype; Goals; Growth; HIV; HIV Core Protein p24; HIV-1; Human Resources; Individual; Institutes; Laboratories; Location; Molecular; Molecular and Cellular Biology; Pathogenesis; Performance; Peripheral Blood Mononuclear Cell; Polymerase Chain Reaction; Productivity; Purpose; Reagent; Research; Research Personnel; SIV; Services; Specific qualifier value; Standards of Weights and Measures; Supervision; Techniques; Technology; Time; Titrations; Training; Training Programs; Use of New Techniques; Viral; Virus; Virus-Cell Membrane Interaction; base; biosafety level 3 facility; cost; expectation; mutant; p27 Cell Cycle Protein; p27 Enzyme Inhibitor; programs; prototype; repository; research study

The purpose of the Viral/Cell/Molecular Core is to provide comprehensive support for virological, molecular and primary cell-based studies, in order enhance the productivity and efficiency of established HIV investigators within the Penn/Wistar/CHOP community, facilitate the entrance of new investigators into the field of AIDS research, and promote scientific collaborations among these investigators. The Core provides: (1) a carefully supervised facility for Biosafety Level 3 facility for the performance of containment experiments by investigators without access to individual containment facilities or who require a level of containment greater than currently available; (2) a central repository of prototype, mutant, and well-characterized primary HIV-1 isolates representing different aspects of pathogenesis; (3) a service for primary HIV-1 isolation, titration and characterization; (4) additional virologic services including growth, titration and QA of viral stocks, and cost-efficient HIV p24 & SIV p27 ELISA assay; (5) high volume primary PBMC of specified genotype (ie, CCR5 delta32 homozygous) by leukapharesis, and; (6) support for the use of quantitative real-time PCR technology including machine use and cost-efficient optimized primer/probe sets and reagents prepared in bulk for Core users. The Core also provides training programs in (a) HIV biosafety practices for lab personnel new to HIV research or refresher programs for current research personnel; (b) training in use of realtime quantitative PCR technology including custom primer development & data analysis software, and; (c) assistance to new investigators with practical aspects of virological techniques. It is our expectation that this Core will continue to enhance productivity and collaborations among existing HIV investigators and facilitate the entrance of non-HIV investigators into the field, while providing a mechanism to enhance laboratory biosafety.

病毒/细胞/分子核心的目的是为病毒学、 基于分子和原代细胞的研究，以提高 Penn/Wistar/CHOP 社区内已建立的 HIV 研究人员的生产力和效率，促进新研究人员进入艾滋病研究领域，并促进这些研究人员之间的科学合作。核心提供：(1) 一个受到严格监督的生物安全 3 级设施，供无法进入个别收容设施或需要高于现有收容水平的调查人员进行收容实验； (2) 代表发病机制不同方面的原型、突变体和特征明确的初级 HIV-1 分离株的中央储存库； (3) HIV-1 初级分离、滴定和表征服务； (4) 其他病毒学服务，包括病毒库存的生长、滴定和 QA，以及经济高效的 HIV p24 和 SIV p27 ELISA 测定； (5)通过白细胞去除术获得指定基因型（即CCR5 delta32纯合子）的高容量原代PBMC， 和; (6) 支持使用定量实时 PCR 技术，包括机器使用和为核心用户批量制备的经济高效的优化引物/探针组和试剂。该核心还为刚接触艾滋病毒研究的实验室人员提供艾滋病毒生物安全实践方面的培训计划，或为现有研究人员提供进修计划； (b) 使用实时定量 PCR 技术（包括定制引物开发和数据分析软件）的培训； (c) 向新研究者提供病毒学技术实用方面的帮助。我们期望该核心将继续提高现有艾滋病毒研究人员之间的生产力和合作，并促进非艾滋病毒研究人员进入该领域，同时提供加强实验室生物安全的机制。