Flow Cytometry

流式细胞仪

• 批准号: 7697011
• 负责人: Allan J Zajac
• 金额: $ 7.14万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-16 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7697011
• 关键词: Acquired Immunodeficiency Syndrome; Address; Arts; Budgets; Cell Separation; Cell physiology; Cells; Color; Communities; Consultations; Coupled; Development; Ensure; Equipment; Flow Cytometry; Goals; HIV; Immune system; Immunity; Individual; Internet; Investigation; Lasers; Measures; Operative Surgical Procedures; Phenotype; Population; Research; Research Activity; Research Personnel; Resources; Services; Techniques; Technology; Training; Training and Education; Update; Vaccines; Viral; Viral Pathogenesis; base; chemical property; cost; instrument; instrumentation; member; novel strategies; tool

The overall objective of the Flow Cytometry Core is to efficiently provide CFAR members with comprehensive flow cytometry services, including equipment, expertise and training at a reasonable cost. Flow cytometry is a unique and powerful technology for determining the phenotype and function of cells and also allows defined populations of cells to be isolated by cell sorting. In recent years the use of flow cytometric analysis in HIV research has become even more prominent as new approaches for dissecting the interactions between HIV and cells of the immune system have been developed. The CFAR Flow Cytometry Core began operation in 1990 and continues to provide flow cytometry services to the AIDS researchers in the UAB community. During the last budget period 82 individual investigators used the flow cytometry core, including 28 CFAR members for AIDS-related projects. The instrumentation in the core has also recently been upgraded and now enables investigators to analyze six different parameters using three laser beams with possible emissions at 360 nm, 407 nm, 488nm, 528 nm, 568 nm, or 633nm. In addition the CFAR Flow Cytometry Core now also has available a FACScan instrument for five parameter, three color analysis. The specific aims of the flow cytometry core are: 1. Service. To provide AIDS Center members with cell sorting and analytical flow cytometry services, which are capable of handling potentially infectious materials. 2. Education and Training. To provide AIDS Center members with up to date information regarding the use of flow cytometry in AIDS research. 3. Development. A major goal of the flow cytometry core is to address users' needs by ensuring that AIDS Center members can take advantage of current technological advances and employ state of the art techniques in their research activities. Overall the CFAR Flow Cytometry Core provides key resources, expertise and support to AIDS investigators at UAB and acts as a focal point for numerous scientific interactions.

流式细胞术核心的总体目标是有效地为 CFAR 成员提供 全面的流式细胞术服务，包括价格合理的设备、专业知识和培训。 流式细胞术是一种独特而强大的技术，用于确定细胞的表型和功能 还允许通过细胞分选分离特定的细胞群。近年来利用流量 随着剖析病毒的新方法的出现，细胞计数分析在艾滋病毒研究中变得更加重要。 HIV 和免疫系统细胞之间的相互作用已经得到发展。 CFAR 流式细胞仪 Core 于 1990 年开始运营，继续为艾滋病研究人员提供流式细胞术服务 阿拉巴马大学社区。在上一个预算期间，82 位独立研究人员使用了流式细胞术核心， 其中包括 28 名 CFAR 艾滋病相关项目成员。核心仪器最近也 已升级，现在使研究人员能够使用三个激光束分析六个不同的参数 可能发射波长为 360 nm、407 nm、488nm、528 nm、568 nm 或 633nm。此外，CFAR 流 Cytometry Core 现在还提供 FACScan 仪器，用于五参数、三色分析。 流式细胞仪核心的具体目标是： 1、服务。为艾滋病中心会员提供细胞分选和分析流式细胞术服务， 能够处理潜在的传染性材料。 2. 教育和培训。向艾滋病中心成员提供有关艾滋病的最新信息 流式细胞术在艾滋病研究中的应用。 3. 发展。流式细胞术核心的一个主要目标是通过确保艾滋病毒感染来满足用户的需求 中心成员可以利用当前的技术进步并采用最先进的技术 他们的研究活动中的技术。 总体而言，CFAR 流式细胞术核心为艾滋病提供了关键资源、专业知识和支持 阿拉巴马大学的研究人员，并充当众多科学互动的焦点。