In Vivo Optical Imaging of Kidney Structure and Function

肾脏结构和功能的体内光学成像

• 批准号: 8334629
• 负责人: Yu Chen
• 金额: $ 11.42万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-20 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8334629
• 关键词: Acute Kidney Failure; Acute Renal Failure with Renal Papillary Necrosis; Animal Model; Animals; Basic Science; Biological Markers; Blood Vessels; Caliber; Clinical; Clinical Medicine; Clinical Research; Contrast Media; Creatinine; Data; Diagnosis; Disease; Disease Progression; Early Diagnosis; Filtration; Fluorescence; Functional Imaging; Functional disorder; Goals; Healthcare; Human; Image; Imaging Device; Imaging technology; Injury; Injury to Kidney; Intensive Care Units; Intervention; Ischemia; Kidney; Kidney Diseases; Kidney Transplantation; Laparoscopy; Measures; Monitor; Morbidity - disease rate; Morphology; Nephrectomy; Optical Coherence Tomography; Outcome; Pathology; Patients; Performance; Physiological; Procedures; Quality of life; Renal Blood Flow; Renal clearance function; Reperfusion Injury; Research Project Grants; Resolution; Serum; Severities; Structure; Supportive care; Technology; Time; Translating; Transplantation; Tubular formation; United States; Urologic Diseases; Warm Ischemia; bench to bedside; clinical practice; delayed graft function; fluorescence imaging; functional status; glomerular filtration; human subject; imaging probe; improved; in vivo; kidney imaging; minimally invasive; molecular imaging; mortality; new technology; novel; novel diagnostics; optical imaging; outcome forecast; renal ischemia; response; success; successful intervention; tool

DESCRIPTION (provided by applicant): The overall goal of this proposal is to develop a minimally-invasive optical imaging technology for the diagnosis of kidney diseases in vivo and in real time. Specifically, in this Pilot and Feasibility Clinical Research Grants in Kidney or Urologic Diseases (R21), we will image the ischemic injury in human subjects during laparoscopic partial nephrectomy procedures to investigate the feasibility of quantitative imaging of the kidney's morphological and functional parameters for assessment of the extent of injury in vivo using multi-parameter optical imaging. Acute kidney injury (AKI) represents a common and potentially devastating disease in clinical medicine with high mortality and morbidity rate. Currently, there is no objective tool for the early diagnosis and real time assessment of the status of AKI. However, timely and appropriate interventions are essential to the potential success and outcome of a therapy. Therefore, there is a critical need for developing new diagnostic tools which can assess the severity and extent of AKI, quantify the physiological parameters of disease, and evaluate disease progression or response to therapy, in vivo and in real time. Such a technology would represent a major advance in the diagnosis and treatment of kidney diseases. This proposal is built upon our unique multi-modal optical imaging platform combining high-resolution optical coherence tomography (OCT) and high-sensitivity fluorescence imaging for simultaneous structural and functional imaging. Our extensive preliminary data on both animal and human kidneys demonstrate the strong potential and promise for optical imaging of kidney pathology. Specifically, we have demonstrated that OCT can provide high-resolution images of the intact human kidney structures including vessels, tubules, and glomeruli; and fluorescence imaging can monitor the dynamics of the glomerular filtration. Together, multi- modal optical imaging provides comprehensive assessment of kidney structural and functional status for more accurate diagnosis and prognosis. To translate this new technology from bench to bedside, we propose to pursue the following three specific aims: 1) Develop a laparoscopic imaging probe for multi-parametric optical imaging of kidney structure and function in vivo. 2) Validate the performance of minimally-invasive multi- parametric imaging on animal model of kidney ischemia. 3) Translate the laparoscopic multi-parametric optical imaging to image human kidney structure and function in vivo during standard laparoscopic procedures. By the end of this proposal, the new minimally-invasive multi-parameter optical imaging technology will be fully developed and its clinical feasibility will be validated. This technology should be ready for the next step larger scale clinical study. This technology will open a new avenue for diagnosis and treatment of kidney diseases including AKI, improve the life quality of patients suffering from these diseases, and positively impact health care throughout the United States.

描述（由申请人提供）：该提案的总体目标是开发一种最小的侵入性光学成像技术来实时诊断肾脏疾病。具体而言，在肾脏或泌尿科疾病（R21）的这一试验和可行性临床研究中，我们将在腹腔镜部分肾切除术过程中成像人类受试者的缺血性损伤，以调查使用跨越多个损伤Insparemeter Inbarameter Inflains Paremeter Inbarame I Imagi-Parame的定量成像的可行性。急性肾脏损伤（AKI）代表了一种常见且潜在的毁灭性疾病，具有高死亡率和发病率的临床医学。当前，没有客观的工具可以对AKI状态进行早期诊断和实时评估。但是，及时和适当的干预措施对于治疗的潜在成功和结果至关重要。因此，迫切需要开发新的诊断工具，这些工具可以评估AKI的严重程度和程度，量化疾病的生理参数，并评估疾病的进展或对治疗，体内和实时的反应。这样的技术将代表肾脏疾病诊断和治疗的重大进步。该建议建立在我们独特的多模式光学成像平台上，结合了高分辨率光学相干断层扫描（OCT）和高敏感性荧光成像，用于同时进行结构和功能成像。我们关于动物和人类肾脏的广泛初步数据表明，肾脏病理学光学成像的强大潜力和希望。具体而言，我们已经证明OCT可以提供完整的人类肾脏结构的高分辨率图像，包括血管，小管和肾小球。荧光成像可以监测肾小球过滤的动力学。总之，多模态光学成像可全面评估肾脏结构和功能状态，以更准确地诊断和预后。为了将这项新技术从长凳转换为床边，我们建议追求以下三个特定目的：1）开发腹腔镜成像探针，用于在体内对肾脏结构和功能的多参数光学成像。 2）验证在肾脏缺血模型上微创多参数成像的性能。 3）将腹腔镜多参数光学成像转换为在标准腹腔镜程序中体内的人体结构和功能图像。 到本提案的结尾，新的最小侵入性多参数光学成像技术将得到充分开发，并将验证其临床可行性。该技术应该为下一步的大规模临床研究做好准备。这项技术将为包括AKI在内的肾脏疾病的诊断和治疗开辟新的途径，改善患有这些疾病的患者的生活质量，并对整个美国的医疗保健产生积极影响。