SPF INDIAN RHESUS MONKEY COLONY FOR AIDS RESEARCH: HIV ANIMAL MODELS

SPF 印度恒河猴艾滋病研究群体：艾滋病毒动物模型

• 批准号: 8173555
• 负责人: JAMES L BLANCHARD
• 金额: $ 26.82万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173555
• 关键词: Acquired Immunodeficiency Syndrome; Animal Model; Animals; Artificial Insemination; Assisted Reproductive Techniques; Biological Assay; Computer Retrieval of Information on Scientific Projects Database; Contracts; Female; Funding; Genetic Variation; Grant; HIV; Housing; Institution; Laboratories; Macaca mulatta; Monitor; Monkeys; Productivity; Research; Research Personnel; Resources; SIV; Screening procedure; Simian Retroviruses; Source; Specific Pathogen Frees; Surface; Testing; Time; United States National Institutes of Health; Validation; Virus; germ free condition; male; pathogen; simian virus; social group; trait

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aim of this application is to establish a colony of Indian rhesus monkeys, free of Simian Immunodeficiency Virus (SIV), simian retrovirus (SRV), herpes b virus, and simian t-lymphotrophic virus (STLV1). This will be accomplished by recruitment of 100 specific pathogen free (SPF) female rhesus monkeys and 15 SPF males in the first year. An additional 50 SPF females and 10 males will be added to the founding colony in each of the subsequent 4 years. We project that by the end of the grant period the colony will have 625 animals. Monkeys will be screened for the presence of the specific pathogens 4 times in the first two years and twice a year after. Social groups will be kept small, 5 to 7 females, and the monkeys will be housed outdoors, in 20ft x 20ft enclosures on natural surfaces. Screening for the presence of the specific pathogens will be done at the Center with validation of assays utilizing commercial labs and NIH funded testing centers. We will incorporate assisted reproductive techniques such as artificial insemination to maximize the productivity of the colony. In addition, we have contracted with established laboratories to genetically monitor the colony for genetic diversity and to identify specific traits that are currently valuable in AIDS research.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 本申请的具体目的是建立一个不含猿免疫缺陷病毒 (SIV)、猿逆转录病毒 (SRV)、b 型疱疹病毒和猿 t 淋巴细胞病毒 (STLV1) 的印度恒河猴群体。这将通过在第一年招募 100 只无特定病原体 (SPF) 雌性恒河猴和 15 只 SPF 雄性恒河猴来实现。在接下来的 4 年里，每年都会有 50 只 SPF 雌性和 10 只雄性被添加到创始群体中。我们预计，到资助期结束时，该群体将拥有 625 只动物。猴子将在头两年内接受 4 次筛查，以确定是否存在特定病原体，之后每年接受两次筛查。社会群体将保持较小规模，即 5 至 7 只雌性，猴子将被安置在户外自然表面上 20 英尺 x 20 英尺的围栏中。特定病原体的存在筛查将在该中心进行，并利用商业实验室和美国国立卫生研究院资助的测试中心对化验进行验证。我们将采用人工授精等辅助生殖技术，以最大限度地提高蜂群的生产力。此外，我们还与已建立的实验室签订了合同，对群体的遗传多样性进行基因监测，并确定目前对艾滋病研究有价值的特定特征。