X-RAY FIBER DIFFRACTION OF THE OPTIC NERVE HEAD & ITS MICROFIBRILLAR COMPONENTS

视神经乳头的 X 射线纤维衍射

• 批准号: 8168632
• 负责人: JENNIFER R LEWIS
• 金额: $ 0.54万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168632
• 关键词: Age; Back; Biomechanics; Blindness; Collagen; Computer Retrieval of Information on Scientific Projects Database; Connective Tissue; Data; Elements; Eye; Fiber; Funding; Glaucoma; Grant; Institution; Intervention; Maps; Modeling; Molecular; Optic Disk; Optic Nerve; Progressive Myopia; Property; Research; Research Personnel; Resources; Risk; Roentgen Rays; Source; Structure; Techniques; Testing; Tissues; United States National Institutes of Health; Vision; X ray diffraction analysis; X-Ray Diffraction; improved; optic nerve disorder

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this project is to use X-Ray fiber diffraction techniques to characterize and map the fibrous connective tissue structure of the back of the eye including the junction with the optic nerve. Comparison of the fiber ultrastructures of healthy (non-progressive myopia and non-glaucoma) versus age-matched tissue with known progressive myopia, glaucoma and optic neuropathy will be examined. The specific aim is to quantify the collagen intermolecular and interfibrillar spacings. The preferred fiber orientation, abundance and organization will be characterized and compared to test for a correlation between these parameters and changes in ocular function in terms of refraction (progressive myopia) or loss of vision (glaucoma and optic neuropathy). Gross changes in the ultrastructures within the tissues of the back of the eye and optic nerve are associated with glaucoma, however the correlation with vision loss is not well understood. Understanding changes in these tissues at the molecular level provided by X-Ray diffraction could improve our understanding of how these collagenous tissues support healthy vision and how any changes could cause vision loss. Characterizing the tissue at this new level could improve our understanding of the structure of the optic nerve and surrounding scleral tissue, as well as identify targets to develop new treatments or interventions, or identify those at risk. These quantitative data will also benefit Finite Element Models that are being developed to understand the biomechanical properties of the back of the eye (Downs et al.).

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 该项目的目的是使用X射线纤维衍射技术来表征和绘制眼睛背面的纤维结缔组织结构，包括与视神经的连接。将检查健康（非促进性近视和非云糖瘤）与具有已知进行性近视，青光眼和视神经神经病变的年龄匹配的组织的纤维超微结构与年龄匹配的组织的比较。具体的目的是量化胶原蛋白间分子间和间间间距。首选的纤维取向，丰度和组织将被表征并进行比较，以测试这些参数与眼功能的相关性，而眼功能的变化在折射（进行性近视）或视力丧失方面（青光眼和视力神经病）。 眼睛背面和视神经的组织内的超微结构的总变化与青光眼有关，但是与视力丧失的相关性尚不清楚。了解这些组织在X射线衍射提供的分子水平上的变化可以提高我们对这些胶原组织如何支持健康视力以及任何变化如何导致视力丧失的理解。 在这个新水平上表征组织可以提高我们对视神经和周围巩膜组织结构的理解，并确定靶标以开发新的治疗方法或干预措施，或者识别有风险的目标。这些定量数据还将受益于正在开发的有限元模型，以了解眼睛背面的生物力学特性（Downs等人）。