NEUROANATOMICAL/FUNCTIONAL CORRELATES IN FASD MODEL

FASD 模型中的神经解剖学/功能相关性

• 批准号: 8171618
• 负责人: Scott Parnell
• 金额: $ 0.55万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171618
• 关键词: Adverse effects; Brain; Brain region; Cerebellum; Computer Retrieval of Information on Scientific Projects Database; Development; Diffusion Magnetic Resonance Imaging; Ethanol; First Pregnancy Trimester; Funding; Goals; Grant; Growth; Hippocampus (Brain); Image; Institution; Knowledge; Learning; Literature; Long-Term Effects; Modeling; Mus; Nervous system structure; Pregnancy; Reporting; Research; Research Personnel; Resolution; Resources; Source; Structure; Time; United States National Institutes of Health; Work; alcohol exposure; behavior test; fetal; postnatal; prenatal; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overall goal of the proposed research is to characterize the long-term effects on the mouse brain resulting from early gestational ethanol exposure. In spite of the tremendous literature describing the actions of ethanol on the developing nervous system, there is still much to learn, especially regarding the effects of ethanol exposure during early periods of pregnancy. Previous studies examining the effects of early gestational ethanol exposure (during parts of the first trimester equivalent) have demonstrated detrimental effects on specific brain regions (e.g. the cerebellum and hippocampus) and a general somatic growth retardation. To date, research concentration has been on the prenatal manifestations of ethanol dysmorphogenesis, with the long-term developmental profile of these adverse effects remaining unexplored. The work outlined in this proposal will utilize high-resolution magnetic resonance imaging (MRI), diffusion tensor imaging (DTI) and a battery of behavioral tests to more fully examine the sequelae of ethanol exposure on gestational day (GD) 8 in C57Bl/6J mice; an exposure time which previous studies have shown to be worthy of in-depth consideration. It is hypothesized that the regional deficits observed and previously reported by the candidate to occur in fetal mice will remain throughout postnatal development, in spite of a reversal of the somatic growth retardation. The results of the experiments proposed herein will greatly expand our knowledge of the long-term effects of early ethanol exposure on both the structure and function of the brain.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 拟议研究的总体目标是表征妊娠早期乙醇暴露对小鼠大脑的长期影响。尽管有大量文献描述了乙醇对发育中的神经系统的作用，但仍有很多东西需要学习，特别是关于怀孕早期接触乙醇的影响。之前的研究检查了妊娠早期接触乙醇（在妊娠前三个月的部分时间）的影响，结果表明对特定大脑区域（例如小脑和海马体）有有害影响，并且会导致全身生长迟缓。迄今为止，研究重点一直集中在乙醇畸形发生的产前表现上，而这些不良影响的长期发育特征仍未得到探索。该提案中概述的工作将利用高分辨率磁共振成像 (MRI)、扩散张量成像 (DTI) 和一系列行为测试来更全面地检查 C57Bl/6J 妊娠第 8 天 (GD) 暴露于乙醇的后遗症老鼠；先前的研究表明，暴露时间值得深入考虑。据推测，尽管体细胞生长迟缓得到了逆转，但候选者观察到和先前报告的在胎儿小鼠中发生的区域缺陷将在整个出生后发育过程中持续存在。本文提出的实验结果将极大地扩展我们对早期乙醇暴露对大脑结构和功能的长期影响的认识。