Project 6: Assessing Adverse Effects of Environmental Hazards on Reproductive

项目 6：评估环境危害对生殖的不利影响

• 批准号: 7936574
• 负责人: BRUCE D HAMMOCK
• 金额: $ 14.82万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7936574
• 关键词: Address; Adrenal Glands; Adult; Adverse effects; Androgens; Animal Model; Area; Biological; Biological Assay; Biological Markers; Breast; Budgets; Clinical; Collaborations; Development; Dibutyl Phthalate; Endocrine Disruptors; Environment; Environmental Hazards; Environmental Risk Factor; Epidemiologic Studies; Estrogen Receptor beta; Evaluation; Exposure to; Future; Growth; Health; Human; Human Cell Line; In Vitro; Individual; Laboratories; Ligands; Macaca; Mammary Gland Parenchyma; Mediating; Modeling; Modification; Nuclear Receptors; Pattern; Perinatal Exposure; Population; Primates; Production; Progesterone Receptors; Receptor Mediated Signal Transduction; Reproduction; Reproductive Health; Research; Risk; Role; Sampling; Screening procedure; Serum; Signal Transduction; System; Thyroid Gland; Toxic Environmental Substances; Toxic effect; Validation; Woman; fetal; hazard; in vivo; nonhuman primate; population based; postnatal; prenatal; prospective; receptor; reproductive; research study; toxicant; triclocarban; Address; Adrenal Glands; Adult; Adverse effects; Androgens; Animal Model; Area; Biological; Biological Assay; Biological Markers; Breast; Budgets; Clinical; Collaborations; Development; Dibutyl Phthalate; Endocrine Disruptors; Environment; Environmental Hazards; Environmental Risk Factor; Epidemiologic Studies; Estrogen Receptor beta; Evaluation; Exposure to; Future; Growth; Health; Human; Human Cell Line; In Vitro; Individual; Laboratories; Ligands; Macaca; Mammary Gland Parenchyma; Mediating; Modeling; Modification; Nuclear Receptors; Pattern; Perinatal Exposure; Population; Primates; Production; Progesterone Receptors; Receptor Mediated Signal Transduction; Reproduction; Reproductive Health; Research; Risk; Role; Sampling; Screening procedure; Serum; Signal Transduction; System; Thyroid Gland; Toxic Environmental Substances; Toxic effect; Validation; Woman; fetal; hazard; in vivo; nonhuman primate; population based; postnatal; prenatal; prospective; receptor; reproductive; research study; toxicant; triclocarban

The over-arching objectives of this project are to develop and apply biomarker assays for evaluating human reproductive health at the population level. Risk to reproductive health is an area of growing public concern. Initially, this project focuses on the development of biomarker assays that can be applied to non-clinical situations and permit epidemiological studies to include prospective evaluations of individual women's reproductive health. The same biomarker assays validated for use in humans are also validated for use in the nonhuman primate animal model because the species-specific aspects of human reproduction often require the use of the laboratory macaque. These assays are then used to conduct in vivo experiments using the non-human primate animal model to fill important gaps in our understanding of specific environmental toxicants including targets of toxicity and exposure risks. The development of biomarkers for effect has subsequently led to the development and validation of biomarker assays for exposure to reproductive health risks and the identification of new environmental toxicants. All assays are adapted to automated platforms so that they are immediately available for use in all research and clinical centers, in vivo experiments conducted to confirm and characterize newly identified reproductive hazards and in vitro experiments using human cells lines are used to develop a deeper understanding of their mechanism(s) of action. Whenever possible, archival biological samples from previous or concurrent epidemiological studies are used in order to pose population-based queries that otherwise could not be addressed within the budget of a single project. Cooperation and collaboration with other projects is emphasized.

该项目的整个目标是开发和应用生物标志物测定法，以评估人群水平的人类生殖健康。生殖健康的风险是公众日益关注的领域。最初，该项目着重于生物标志物测定法的开发，这些测定法可以应用于非临床情况，并允许流行病学研究包括对个别女性生殖健康的前瞻性评估。同样的生物标志物测定法用于人类使用，也经过验证用于非人类灵长类动物模型，因为人类繁殖的物种特定方面经常 需要使用实验室猕猴。然后，这些测定法用于使用非人类灵长类动物模型进行体内实验，以填补我们对特定环境毒物的理解，包括毒性和暴露风险。生物标志物的生物标志物的发展随后导致了生物标志物测定的开发和验证，以暴露于生殖健康风险和鉴定新的环境有毒物质。所有测定都适用于 自动化平台，以便在所有研究和临床中心，在体内实验中可立即使用，以确认和表征新鉴定的新鉴定的生殖危害以及使用人类细胞系的体外实验来对其作用机制有更深入的了解。只要有可能，就使用以前或同时流行病学研究的档案生物学样本，以提出基于人群的查询，否则这些查询无法在单个项目的预算中解决。强调与其他项目的合作与合作。