EFFECT OF OXIDATIVE STRESS ON SULFATE AND MOLYBDENUM IN GREEN ALGAE

氧化应激对绿藻中硫酸盐和钼的影响

• 批准号: 8167623
• 负责人: ERICA ODUARAN
• 金额: $ 0.58万
• 依托单位: UNIVERSITY OF RHODE ISLAND
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167623
• 关键词: Acetates; Algae; Assimilations; Carbon; Carbon Dioxide; Cell Aging; Cells; Chlamydomonas reinhardtii; Chloroplasts; Complex; Computer Retrieval of Information on Scientific Projects Database; Environment; Funding; Grant; Green Algae; Inorganic Sulfates; Institution; Metabolic; Metals; Micronutrients; Mitochondria; Molybdenum; Organism; Oxidative Stress; Research; Research Personnel; Resources; Source; Sunlight; Temperature; United States National Institutes of Health; Unspecified or Sulfate Ion Sulfates; cell age; cell injury; design; light intensity; oxidative damage; repaired; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Because oxidative damage results in aging, cells have a variety of mechanisms to protect against oxidative damage and to repair themselves once damage is done. Proper storage and sequestration of micronutrients, such as metals, is essential to protecting cells from damage when they come under stressful conditions. Since the transport, storage and use of micronutrients is more complex in multicellular organisms, the tactics used by unicellular organisms are being investigated in this study with hopes of extrapolating the findings to larger more complex organisms. The green algae Chlamydomonas reinhardtii adapts well to its environment and can grow under various conditions of salinity, moisture, temperature, light intensity and micronutrient availability. Part of its versatility comes from that fact that it is able to grow heterotrophically in the dark with acetate as its reduced carbon source or to grow phototrophically with sunlight and carbon dioxide. This demonstrates the fact that C. reinhardtii can use either the chloroplasts or mitochondria in response to metabolic demands and raises the question of how the micronutrients are allocated in the cell with respect to metabolic demand. The algae have several protective and preventative mechanisms for avoiding oxidative damage when faced with adverse conditions. What is not understood is how the essential metals and micronutrients are transported, sequestered, stored or re-allocated when cells are confronted with these stressful conditions. This project is designed to probe the assimilation of the micronutrient molybdenum when C. reinhardtii is under conditions of oxidative stress.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 由于氧化损伤会导致衰老，因此细胞有多种机制来防止氧化损伤，并在损伤发生后进行自我修复。适当储存和隔离金属等微量营养素对于保护细胞在压力条件下免受损害至关重要。由于多细胞生物体中微量营养素的运输、储存和使用更加复杂，因此本研究正在研究单细胞生物体使用的策略，希望将研究结果外推到更大更复杂的生物体。 绿藻莱茵衣藻能够很好地适应其环境，可以在各种盐度、湿度、温度、光照强度和微量营养素可用性条件下生长。其多功能性部分来自于这样的事实：它能够在黑暗中以醋酸盐作为其减少的碳源异养生长，或者以阳光和二氧化碳光养生长。这表明莱茵衣藻可以利用叶绿体或线粒体来响应代谢需求，并提出了微量营养素如何根据代谢需求在细胞中分配的问题。藻类具有多种保护和预防机制，可以在面临不利条件时避免氧化损伤。目前尚不清楚的是，当细胞面临这些压力条件时，必需金属和微量营养素是如何运输、隔离、储存或重新分配的。该项目旨在探讨莱茵衣藻处于氧化应激条件下时微量营养素钼的同化情况。