MOLECULAR MECHANISMS OF MRNA STABILITY IN HUMAN SALIVA

人类唾液中 mRNA 稳定性的分子机制

• 批准号: 8167773
• 负责人: Viswanathan Palanisamy
• 金额: $ 21.31万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167773
• 关键词: Biological Markers; Cancer Patient; Computer Retrieval of Information on Scientific Projects Database; Disease; Elements; Funding; Gene Expression Regulation; Goals; Grant; Human; Institution; Knowledge; MAP Kinase Activation Pathway; Messenger RNA; Molecular; Pathway interactions; Play; Proteins; RNA; Regulation; Research; Research Personnel; Resources; Role; Saliva; Source; United States National Institutes of Health; Viral; insight; mRNA Decay; mRNA Stability; malignant mouth neoplasm; Biological Markers; Cancer Patient; Computer Retrieval of Information on Scientific Projects Database; Disease; Elements; Funding; Gene Expression Regulation; Goals; Grant; Human; Institution; Knowledge; MAP Kinase Activation Pathway; Messenger RNA; Molecular; Pathway interactions; Play; Proteins; RNA; Regulation; Research; Research Personnel; Resources; Role; Saliva; Source; United States National Institutes of Health; Viral; insight; mRNA Decay; mRNA Stability; malignant mouth neoplasm

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Stabilization and destabilization of mRNA plays an important role in the regulation of gene expression. Our knowledge of presence of human mRNA and the stabilization that enables its presence in human saliva is very poor. The existence of viral, bacterial and human RNAs in saliva allows us to study the mechanisms and regulations of mRNA stability for various diseases. The challenges related to regulation of mRNA stability in human saliva, and how mRNA is stabilized by utilizing mRNA stability pathways is yet to uncover. We hypothesize that there are sequence elements and protein factors responsible for the differential presence of disease specific RNA biomarkers in saliva or oral cancer patients. This research will allow us to gain insights into the sequence elements and protein factors responsible for mRNA stability in human saliva. Therefore, the major goal of this proposal is to understand the mechanistic aspects of mRNA stability in human saliva. Two specific aims are proposed to accomplish these goals. I) Determine the fundamental mechanisms responsible for the stabilization of mRNA's in human saliva and II) Validate the AU-rich element containing mRNA decay in correlation with MAP kinase pathway activation.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 mRNA的稳定和不稳定在基因表达的调节中起重要作用。 我们对人类mRNA的存在以及使其在人类唾液中存在的稳定非常贫穷。唾液中病毒，细菌和人类RNA的存在使我们能够研究各种疾病的mRNA稳定性的机制和法规。 尚未发现与人类唾液中mRNA稳定性有关的挑战，以及如何通过使用mRNA稳定性途径稳定mRNA的挑战尚未发现。我们假设有一些序列元素和蛋白质因子，导致唾液或口腔癌患者中疾病特异性RNA生物标志物的差异存在。这项研究将使我们能够深入了解负责人唾液中mRNA稳定性的序列元素和蛋白质因子。因此，该提案的主要目标是了解人类唾液中mRNA稳定性的机械方面。提出了两个具体目标来实现这些目标。 i）确定负责人唾液中mRNA稳定的基本机制和ii）验证含有与MAP激酶途径激活的MAP激酶途径相关的富含AU元素。