SENSORS FOR ANIONS OF ENVIRONMENTAL AND BIOMEDICAL RELEVANCE

环境和生物医学相关阴离子传感器

• 批准号: 8357070
• 负责人: Md. Alamgir Hossain
• 金额: $ 10.41万
• 依托单位: JACKSON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357070
• 关键词: Achievement; Acidity; Affinity; Amides; Anions; Binding; Binding Sites; Biological Assay; Biological Products; Biology; Bromides; Carcinoma; Cations; Cells; Charge; Chemistry; Chloroform; Complex; Crown Ethers; Data; Development; Devices; Dimensions; Dimethyl Sulfoxide; Electrostatics; Environment; Environment and Public Health; Environmental Health; Fluorescence; Foundations; Free Energy; Goals; Health; Human; Hydrogen Bonding; Inorganic Sulfates; Life; Ligands; Link; Liver; Malignant neoplasm of thyroid; Medical; Methanol; Mole the mammal; Molecular Mechanisms of Action; Naphthalene; Nitrates; Optics; Peptides; Perchlorates; Physiological; Play; Process; Property; Public Health; Pyrenes; Radial; Raman Spectrum Analysis; Research; Roentgen Rays; Role; Sensory; Series; Signal Transduction; Solutions; Solvents; Structure; Sulfur; System; Techniques; Thioamides; Titrations; Universities; Unspecified or Sulfate Ion Sulfates; Water; X-Ray Crystallography; base; cancer cell; carboxylate; cytotoxicity; design; fluorophore; functional group; improved; inorganic phosphate; optical sensor; prototype; pyridine; receptor; sensor; solid state

THIOAMIDE-BASED SENSOR FOR ANIONS OF ENVIRONMENTAL AND BIOMEDICAL RELEVANCE Selective binding of anions occurs in numerous physiological and enzymatic processes in life and is of paramount importance for many applications such as potential prototypes of new sensory devices, new extraction agents, and biological carriers. Surprisingly, synthetic receptors and sensors for anions are still very limited. The proposal will focus on developing new anion receptors, binders and sensors, which will have application in improving human health and the environment. The project is based on the hypothesis that the introduction of (C=S)NH, as a binding site to a synthetic receptor, enhances anion binding ability, and a fluorophore group if linked to the molecule displays a detectable optical change in presence of an anion. The long term goal of this project is to provide strategies for the design of highly selective sensor systems, and thus it will add a new dimension to the field of anion sensor chemistry. In Specific Aim 1, a new series of thioamide-based receptors with different spacers and variable rigidity will be synthesized, which include (1) acyclic thioamide receptors with TREN, aromatic and crown ether units, (ii) macrocyclic thioamide receptors with variable spacers, and (iii) optical sensors containing pyrene and dansyl groups. Series (i) and (ii) will bind anions through (C=S)NH...X- interactions, while the signaling unit(s) in series (iii) will serve as anion sensing. Specific Aim 2 is designed to investigate the binding properties of the synthesized molecules for anions in different solvents. The target anions are sulfate, phosphate, nitrate, perchlorate and halides, which are directly related to environment health and public health. Specific Aim 3 will focus on isolating complexes of anions bound to our synthetic ligands, and determining the structure of those complexes by X-ray crystallography. In Specific Aim 4, the cytotoxicity of thioamide-based ligands will be studied in both human liver carcinoma and human thyroid cancer cells using the MTT assay. Successful completion of this project will contribute greatly to the development of a framework for environmental and biomedical applications, and to the achievement the overall goals of RCMI at Jackson State University.

基于硫代酰胺的阴离子传感器 环境和生物医学相关性 阴离子的选择性结合发生在生命中的许多生理和酶促过程中，并且具有重要意义。 对于许多应用来说至关重要，例如新传感设备的潜在原型、新 提取剂和生物载体。令人惊讶的是，阴离子的合成受体和传感器是 仍然非常有限。该提案将重点开发新的阴离子受体、粘合剂和传感器， 这将应用于改善人类健康和环境。该项目基于 假设引入 (C=S)NH 作为合成受体的结合位点，可增强 阴离子结合能力，并且荧光团如果与分子连接则显示出可检测的光学 阴离子存在时发生变化。该项目的长期目标是为 高选择性传感器系统的设计，将为负离子领域增添新的维度 传感器化学。在特定目标 1 中，具有不同间隔基的新系列硫代酰胺基受体 将合成可变刚性，其中包括（1）带有TREN的无环硫代酰胺受体， 芳香族和冠醚单元，（ii）具有可变间隔基的大环硫代酰胺受体，和（iii） 含有芘和丹酰基的光学传感器。系列（i）和（ii）将通过结合阴离子 (C=S)NH...X-相互作用，而系列(iii)中的信号单元将充当阴离子传感。具体的 目标 2 旨在研究合成分子与阴离子的结合特性 不同的溶剂。目标阴离子是硫酸根、磷酸根、硝酸根、高氯酸根和卤化物，它们是 直接关系到环境卫生和公共卫生。具体目标 3 将侧重于隔离 与我们的合成配体结合的阴离子复合物，并确定这些复合物的结构 通过X射线晶体学。在具体目标 4 中，将研究硫代酰胺基配体的细胞毒性 使用 MTT 测定在人肝癌和人甲状腺癌细胞中进行检测。成功的 该项目的完成将极大地促进环境框架的制定 和生物医学应用，并实现杰克逊州立大学 RCMI 的总体目标 大学。