SENSORS FOR ANIONS OF ENVIRONMENTAL AND BIOMEDICAL RELEVANCE

环境和生物医学相关阴离子传感器

• 批准号: 8357070
• 负责人: Md. Alamgir Hossain
• 金额: $ 10.41万
• 依托单位: JACKSON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357070
• 关键词: Achievement; Acidity; Affinity; Amides; Anions; Binding; Binding Sites; Biological Assay; Biological Products; Biology; Bromides; Carcinoma; Cations; Cells; Charge; Chemistry; Chloroform; Complex; Crown Ethers; Data; Development; Devices; Dimensions; Dimethyl Sulfoxide; Electrostatics; Environment; Environment and Public Health; Environmental Health; Fluorescence; Foundations; Free Energy; Goals; Health; Human; Hydrogen Bonding; Inorganic Sulfates; Life; Ligands; Link; Liver; Malignant neoplasm of thyroid; Medical; Methanol; Mole the mammal; Molecular Mechanisms of Action; Naphthalene; Nitrates; Optics; Peptides; Perchlorates; Physiological; Play; Process; Property; Public Health; Pyrenes; Radial; Raman Spectrum Analysis; Research; Roentgen Rays; Role; Sensory; Series; Signal Transduction; Solutions; Solvents; Structure; Sulfur; System; Techniques; Thioamides; Titrations; Universities; Unspecified or Sulfate Ion Sulfates; Water; X-Ray Crystallography; base; cancer cell; carboxylate; cytotoxicity; design; fluorophore; functional group; improved; inorganic phosphate; optical sensor; prototype; pyridine; receptor; sensor; solid state

THIOAMIDE-BASED SENSOR FOR ANIONS OF ENVIRONMENTAL AND BIOMEDICAL RELEVANCE Selective binding of anions occurs in numerous physiological and enzymatic processes in life and is of paramount importance for many applications such as potential prototypes of new sensory devices, new extraction agents, and biological carriers. Surprisingly, synthetic receptors and sensors for anions are still very limited. The proposal will focus on developing new anion receptors, binders and sensors, which will have application in improving human health and the environment. The project is based on the hypothesis that the introduction of (C=S)NH, as a binding site to a synthetic receptor, enhances anion binding ability, and a fluorophore group if linked to the molecule displays a detectable optical change in presence of an anion. The long term goal of this project is to provide strategies for the design of highly selective sensor systems, and thus it will add a new dimension to the field of anion sensor chemistry. In Specific Aim 1, a new series of thioamide-based receptors with different spacers and variable rigidity will be synthesized, which include (1) acyclic thioamide receptors with TREN, aromatic and crown ether units, (ii) macrocyclic thioamide receptors with variable spacers, and (iii) optical sensors containing pyrene and dansyl groups. Series (i) and (ii) will bind anions through (C=S)NH...X- interactions, while the signaling unit(s) in series (iii) will serve as anion sensing. Specific Aim 2 is designed to investigate the binding properties of the synthesized molecules for anions in different solvents. The target anions are sulfate, phosphate, nitrate, perchlorate and halides, which are directly related to environment health and public health. Specific Aim 3 will focus on isolating complexes of anions bound to our synthetic ligands, and determining the structure of those complexes by X-ray crystallography. In Specific Aim 4, the cytotoxicity of thioamide-based ligands will be studied in both human liver carcinoma and human thyroid cancer cells using the MTT assay. Successful completion of this project will contribute greatly to the development of a framework for environmental and biomedical applications, and to the achievement the overall goals of RCMI at Jackson State University.

基于硫酰胺的传感器 环境和生物医学相关性 阴离子的选择性结合发生在生活中的许多生理和酶促过程中 对于许多应用的重要性，例如新感觉设备的潜在原型，新的 提取剂和生物载体。令人惊讶的是，阴离子的合成受体和传感器是 仍然非常有限。该提案将着重于开发新的阴离子受体，粘合剂和传感器， 它将在改善人类健康和环境中应用。该项目基于 假设（C = S）NH作为合成受体的结合位点的引入增强了 阴离子结合能力，如果链接到分子，则显示可检测的光学 在存在阴离子的情况下变化。该项目的长期目标是为 高度选择性传感器系统的设计，因此它将为阴离子的领域添加一个新的维度 传感器化学。在特定目标1中，一系列具有不同垫片的新系列基于硫酰胺的受体 将合成可变的刚度，其中包括（1）带有Tren的无环硫酰胺受体， 芳族和冠醚单元，（ii）带有变速剂的大环硫酰胺受体，以及（iii） 含有吡啶和丹苯基的光学传感器。系列（i）和（ii）将通过 （C = S）NH ... X-相互作用，而（III）中的信号单元（S）将作为阴离子传感。具体的 AIM 2旨在研究阴离子中合成分子的结合特性 不同的溶剂。目标阴离子是硫酸盐，磷酸盐，硝酸盐，高氯酸盐和卤化物，它们是 与环境健康和公共卫生直接相关。特定目标3将集中于隔离 阴离子的复合物与我们的合成配体结合，并确定这些复合物的结构 X射线晶体学。在特定的目标4中，将研究基于硫酰胺的配体的细胞毒性 在人肝癌和人类甲状腺癌细胞中，使用MTT分析。成功的 该项目的完成将有助于开发环境框架 和生物医学应用，以及成就杰克逊州RCMI的总体目标 大学。