MOTOR EFFECTS DERMAL FIBROBLAST GRAFTS IN GLOBUS PALLIDUS- PARKINSONIAN PRIMATES

苍白球-帕金森病灵长类动物真皮成纤维细胞移植物的运动效应

• 批准号: 8357537
• 负责人: Stella M Papa
• 金额: $ 3.29万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357537
• 关键词: Animals; Basal Ganglia; Behavior; Biopsy; Cultured Cells; Dermal; Dyskinetic syndrome; Fibroblasts; Funding; Globus Pallidus; Grant; Levodopa; Location; Maps; Microelectrodes; Monkeys; Motor; National Center for Research Resources; Neurons; Parkinson Disease; Parkinsonian Disorders; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Rodent Model; Skin; Source; Symptoms; Testing; Transplantation; United States National Institutes of Health; behavior test; cost; improved; Animals; Basal Ganglia; Behavior; Biopsy; Cultured Cells; Dermal; Dyskinetic syndrome; Fibroblasts; Funding; Globus Pallidus; Grant; Levodopa; Location; Maps; Microelectrodes; Monkeys; Motor; National Center for Research Resources; Neurons; Parkinson Disease; Parkinsonian Disorders; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Rodent Model; Skin; Source; Symptoms; Testing; Transplantation; United States National Institutes of Health; behavior test; cost; improved

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Dermal fibroblasts grafted in the globus pallidus have been shown to modulate the neuronal activity and improve motor behavior in rodent models of Parkinson's disease. In this project, we will study the transplantation of fibroblasts into the globus pallidus of parkinsonian monkeys to evaluate effects on parkinsonian symptoms. We plan to obtain fibroblast from skin biopsy and subsequently culture the cells for grafting. In aim 1, we plan to test the accuracy of location of the graft using microelectrode-guided mapping of basal ganglia regions in a normal monkey. In Aim 2, the fibroblast transplantation will be carried out in MPTP parkinsonian monkeys. In these parkinsonian monkeys, tests of levodopa-induced dyskinesias will be preformed for three months after the transplantation. These animals will be sacrificed at the end of the behavioral testing period. This project has just started with Aim 1.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 已证明在帕利德斯球骨中移植的真皮成纤维细胞可调节神经元活性并改善帕金森氏病啮齿动物模型中的运动行为。在这个项目中，我们将研究将成纤维细胞移植到帕金森尼猴子的球pallidus，以评估对帕金森氏症状的影响。我们计划从皮肤活检中获得成纤维细胞，然后培养细胞进行嫁接。在AIM 1中，我们计划使用正常猴子中基底神经节区域的微电极引导的映射测试移植物位置的准确性。在AIM 2中，成纤维细胞移植将在MPTP Parkinsonian猴子进行。 在这些帕金森尼猴子中，移植后三个月将对左旋多巴诱导的运动障碍的测试进行预先形成。这些动物将在行为测试期结束时处死。该项目刚刚从AIM 1开始。