ALTERATIONS IN LIVER, MUSCLE, AND ADIPOSE TISSUE INSULIN SENSITIVITY

肝脏、肌肉和脂肪组织胰岛素敏感性的改变

• 批准号: 7355257
• 负责人: Dominic N Reeds
• 金额: $ 1.39万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7355257
• 关键词: ALTERATIONS; LIVER; MUSCLE; ADIPOSE; TISSUE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Alterations in liver, muscle, and adipose tissue insulin sensitivity in men with HIV infection and dyslipidemia: Dyslipidemia is common in patients with HIV infection. In this study, a two-stage euglycemic, hyperinsulinemic clamp, with infusion of stable isotopically labeled tracers, was used to evaluate insulin action in skeletal muscle, liver and adipose tissue in HIV-infected men with dyslipidemia (HIV-DL; plasma triglyceride >250mg/dl and HDL<45mg/dl; n=12), HIV-infected men without dyslipidemia (HIV w/o DL; n=12), and healthy men (n=6). Basal rates of glucose production (glucose Ra), glucose disposal (glucose Rd), and lipolysis (palmitate Ra) were similar between groups. The relative suppression of glucose Ra (63¿4%, 77¿2%, and 78¿3%; P=0.008) and palmitate Ra (49¿4%, 63¿3%, and 68¿3%; P=0.005) during low-dose insulin infusion (plasma insulin=30 ¿U/ml), and the relative stimulation of glucose Rd (214¿21%, 390¿25% and 393¿46%; P=0.001) during high-dose insulin infusion (plasma insulin=75¿U/ml) were lower in HIV-DL than in HIV w/o DL and healthy volunteers, respectively. Suppression of basal glucose Ra correlated with plasma adiponectin (r =0.44, P=0.02), and inversely with plasma interleukin-6 (r =-0.49, P<0.001). Stimulation of glucose Rd correlated directly with adiponectin (r=0.48, P<0.01) and inversely with IL-6 (r=-0.49, P=0.02). We conclude that dyslipidemia in HIV-infected men is indicative of multi-organ insulin resistance, and circulating adipokines may be important in the pathogenesis of impaired insulin action.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一，该子项目和研究者 (PI) 可能已从其他 NIH 来源获得主要资助，因此可以在其他 CRISP 机构中得到体现。列出的是该中心，该中心不一定是研究者所在的机构。 患有 HIV 感染组织和血脂异常的男性的肝脏、肌肉和脂肪胰岛素敏感性发生变化：血脂异常在 HIV 感染患者中很常见。研究采用两阶段血糖正常、高胰岛素钳夹，并注入稳定同位素标记的示踪剂，用于评估患有血脂异常的 HIV 感染男性（HIV-DL；血浆甘油三酯 >250mg/ dl 和 HDL<45mg/dl；n=12），无血脂异常的 HIV 感染男性（HIV w/o DL； n=12) 和健康男性 (n=6) 的基础葡萄糖生成率 (葡萄糖 Ra)、葡萄糖消耗率 (葡萄糖 Rd) 和脂肪分解率 (棕榈酸 Ra) 相似。葡萄糖 Ra 的相对抑制 (63)。 �4%、77�2% 和 78�3%；P=0.008) 和棕榈酸酯 Ra (49�4%、63�3% 和68¿3%；P=0.005) 在低剂量胰岛素输注期间（血浆胰岛素=30¿ U/ml），高剂量胰岛素输注（血浆胰岛素=75¿U/ml）期间葡萄糖Rd的相对刺激（214¿21%、390¿25%和393¿46%；P=0.001）较低HIV-DL 中的基础血糖 Ra 抑制与血浆脂联素相关（r = 0.44，葡萄糖刺激 Rd 与脂联素直接相关（r=0.48，P<0.01），与 IL-6 成反比（r=-0.49，P<0.01）。 0.49，P=0.02），我们得出结论，HIV 感染男性的血脂异常表明存在多器官胰岛素抵抗，并且循环脂肪因子可能在胰岛素作用受损的发病机制中发挥重要作用。