Engineering a new biomaterial for stem-cell mediated bone regeneration

设计用于干细胞介导的骨再生的新型生物材料

• 批准号: 8098914
• 负责人: Ching-Chang Ko
• 金额: $ 12.69万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8098914
• 关键词: Adhesions; Adult; Affect; Animal Model; Architecture; Area; Artificial Liver; Autologous Transplantation; Biochemistry; Biocompatible Materials; Biology; Biomechanics; Biomimetics; Bone Regeneration; Bone Tissue; Cell Count; Cell Differentiation process; Cell Therapy; Cells; Cephalic; Ceramics; Characteristics; Chemical Engineering; Chemistry; Clinical; Clinical Research; Clinical Treatment; Clinical Trials; Collagen; Compressive Strength; Congenital Abnormality; Country; Data; Defect; Deformity; Development; Differentiation Antigens; Drug Delivery Systems; Endothelial Cells; Engineering; Excision; FDA approved; Face; Failure; Family suidae; Fracture; Gelatin; Gene Expression; Gene Targeting; Genes; Genetic; Goals; Growth; Growth Factor; Hand; Hepatocyte; Hour; Human; Hydroxyapatites; Implant; In Vitro; Intervention; Jaw; Joints; Kinetics; Laboratories; Liposomes; Longitudinal Studies; Mechanics; Mediating; Mentors; Molecular; Multipotent Stem Cells; Natural regeneration; Non-Viral Vector; Open Fractures; Operative Surgical Procedures; Orthodontics; Osteoblasts; Osteoclasts; Patients; Penetration; Pharmaceutical Preparations; Pharmacy (field); Phase; Physiological; Plant Leaves; Polymers; Porosity; Powder dose form; Procedures; Process; Property; Proteins; Research; Research Personnel; Research Project Grants; ST14 gene; Science; Scientist; Solid; Solutions; Source; Stem cells; Structure; Technology; Testing; Time; Tissue Engineering; Tissues; Training; Training Programs; Translating; Transplanted tissue; Trauma; Vesicle; Water; Weight-Bearing state; Work; Wound Healing; Xenograft procedure; base; biodegradable polymer; bone; bone cell; bone morphogenic protein; career development; cell growth; cellular engineering; controlled release; craniofacial; craniofacial complex; dentofacial; design; experience; follow-up; gene therapy; improved; in vivo; innovation; nano; nanocomposite; novel; orthognathic; physical property; programs; repaired; scaffold; skills; stem cell biology; tumor; vector

DESCRIPTION (provided by applicant): In a 4-year training/career development program for this investigator who has a strong background in biomaterials science and more recent clinical training in orthodontics, three specific aims are: (1) the development of further skills in tissue engineering involving a porous scaffold for ingrowth of tissues, extending previous experience and expertise in this area; (2) new capabilities in use of MAPC (multipotent adult progenitor cells) in tissue engineering of this type; and (3) clinical research skills based on longitudinal follow-up of patients treated for various types of dentofacail and craniofacial deformities. The overall longterm goal is to develop a replacement for both soft and hard tissue defects in the craniofacial complex (and elsewhere in the body). The short-term goal is to determine whether a biomimetic hydroxyapatite-gelatin (HAP-GEL) nanocomposite can serve as a load bearing scaffold to carry MAPC and grow bone. The training program is built around interaction with four mentors, related to each specific aim above, and participation in courses and seminars developed in the clinical scientist program at UNC. The mentors are Drs. Mitsuo Yamauchi (application of collagen cross-linkage in scaffold development for tissue engineering), Wei-Shou Hu (stem cell biology) and Leaf Huang (application of growth factors) and William Proffit (longitudinal studies of patients requiring orthognathic surgery for correction of dentofacial and craniofacial defects). Proposed work toward the short-term goal involves (1) cultivation of MAPC on a nanocomposite HAPGEL matrix so that MAPC differentiate into bone cells; (2) design of the HAP-GEL matrix to allow controlled release of growth factors to produce multiple lineages of MAPC differentiation and maximize penetration of MAPC into the matrix; and (3) design of the matrix to maximize the mechanical strength of the MAPCcarrying scaffold so that it could become a load-carrying graft. Preliminary data show promising interactions between osteoblasts and the HAP-GEL matrix, and it appears that this offers a highly favorable way to offer MAPC-based therapy. The significance of this work is that it can provide a new way to replace missing tissues in patients with congenital, developmental or trauma-induced deformities. With a graft material of this type, it should be possible to eliminate multiple surgeries in the treatment of critical-size cranial and facial defects.

项目成果

Osteogenic Potential of Multipotent Adult Progenitor Cells for Calvaria Bone Regeneration.

• DOI: 10.1155/2016/2803081
• 发表时间: 2016
• 期刊: Advances in medicine
• 作者: Lee DJ;Park Y;Hu WS;Ko CC
• 通讯作者: Ko CC

Biological assessment of a calcium silicate incorporated hydroxyapatite-gelatin nanocomposite: a comparison to decellularized bone matrix.

• DOI: 10.1155/2014/837524
• 发表时间: 2014
• 期刊: BioMed research international
• 作者: Lee DJ;Padilla R;Zhang H;Hu WS;Ko CC
• 通讯作者: Ko CC

Effect of light-curing units on the thermal expansion of resin nanocomposites.

光固化单元对树脂纳米复合材料热膨胀的影响。

• 发表时间: 2010
• 期刊: American journal of dentistry
• 影响因子: 1.4
• 作者: Park,Jeong-Kil;Hur,Bock;Ko,Ching-Chang;García-Godoy,Franklin;Kim,Hyung-Il;Kwon,YongHoon
• 通讯作者: Kwon,YongHoon

Influence of DPSS laser on polymerization shrinkage and mass change of resin composites.

DPSS激光对树脂复合材料聚合收缩和质量变化的影响。

• DOI: 10.1089/pho.2010.2897
• 发表时间: 2011
• 期刊: Photomedicine and laser surgery
• 作者: Shin,Dong-Hee;Yun,Dong-In;Park,Mi-Gyong;Ko,Ching-Chang;Garcia-Godoy,Franklin;Kim,Hyung-Il;Kwon,YongHoon
• 通讯作者: Kwon,YongHoon

Polydopamine-Laced Biomimetic Material Stimulation of Bone Marrow Derived Mesenchymal Stem Cells to Promote Osteogenic Effects.

• DOI: 10.1038/s41598-017-13326-y
• 发表时间: 2017-10-11
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Lee DJ;Lee YT;Zou R;Daniel R;Ko CC
• 通讯作者: Ko CC