InstaCortisol:A realtime and continuous assessment of Cortisol in ISF

InstaCortisol：ISF 中皮质醇的实时、连续评估

• 批准号: 7992743
• 负责人: Manju Grover Venugopal
• 金额: $ 0.67万
• 依托单位: GUIDED THERAPEUTICS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7992743
• 关键词: Acquired Immunodeficiency Syndrome; Adrenal Cortex; Age; Alkaline Phosphatase; Antibodies; Antineoplastic Agents; Behavior; Behavioral Sciences; Benchmarking; Biological Assay; Biological Markers; Biological Process; Biosensor; Calibration; Categories; Clinical; Data; Data Collection; Detection; Devices; Diabetes Mellitus; Diet; Digestion; Disease; Drug Addiction; Elderly; Environment; Enzymes; Epinephrine; Error Sources; Etiology; Evaluation; Exercise; Exposure to; Frequencies; Genetic; Hand; Harvest; Healed; Health; Heart Diseases; Hormones; Hour; Household; Housing; Human; Hydrocortisone; Hypertension; Immune system; Individual; Intercellular Fluid; Intervention; Learning; Life; Life Style; Major Depressive Disorder; Malignant Neoplasms; Marketing; Measurement; Measures; Mental Depression; Metabolism; Methods; Monitor; Muscle; Natural regeneration; Nicotine; Normal Cell; Obesity; Osteoporosis; Participant; Patient Self-Report; Patients; Pattern; Performance; Phase; Physical environment; Physiological; Play; Process; Production; Psychosocial Stress; Public Health; Reaction; Research; Research Personnel; Role; Salivary; Sampling; Sensitivity and Specificity; Signal Transduction; Sleep; Smoker; Social Interaction; Socioeconomic Status; Sports Medicine; Steroids; Stress; Surveys; System; Technology; Testing; Time; Weight Gain; Wireless Technology; Wrist; base; bone; cross reactivity; dehydroepiandrosterone; design; detector; experience; field study; healing; human disease; improved; innovation; meetings; minimally invasive; operation; physical conditioning; psychosocial; public health relevance; research study; sensor; strength training; stressor; transmission process; virtual; Acquired Immunodeficiency Syndrome; Adrenal Cortex; Age; Alkaline Phosphatase; Antibodies; Antineoplastic Agents; Behavior; Behavioral Sciences; Benchmarking; Biological Assay; Biological Markers; Biological Process; Biosensor; Calibration; Categories; Clinical; Data; Data Collection; Detection; Devices; Diabetes Mellitus; Diet; Digestion; Disease; Drug Addiction; Elderly; Environment; Enzymes; Epinephrine; Error Sources; Etiology; Evaluation; Exercise; Exposure to; Frequencies; Genetic; Hand; Harvest; Healed; Health; Heart Diseases; Hormones; Hour; Household; Housing; Human; Hydrocortisone; Hypertension; Immune system; Individual; Intercellular Fluid; Intervention; Learning; Life; Life Style; Major Depressive Disorder; Malignant Neoplasms; Marketing; Measurement; Measures; Mental Depression; Metabolism; Methods; Monitor; Muscle; Natural regeneration; Nicotine; Normal Cell; Obesity; Osteoporosis; Participant; Patient Self-Report; Patients; Pattern; Performance; Phase; Physical environment; Physiological; Play; Process; Production; Psychosocial Stress; Public Health; Reaction; Research; Research Personnel; Role; Salivary; Sampling; Sensitivity and Specificity; Signal Transduction; Sleep; Smoker; Social Interaction; Socioeconomic Status; Sports Medicine; Steroids; Stress; Surveys; System; Technology; Testing; Time; Weight Gain; Wireless Technology; Wrist; base; bone; cross reactivity; dehydroepiandrosterone; design; detector; experience; field study; healing; human disease; improved; innovation; meetings; minimally invasive; operation; physical conditioning; psychosocial; public health relevance; research study; sensor; strength training; stressor; transmission process; virtual

DESCRIPTION (provided by applicant): "StressSmart" Repeated exposures to psychosocial stress are associated with several human diseases (e.g., cancer, drug addiction, heart disease, depression) of tremendous public health burden. However, our progress is limited by the lack of a portable device that can enable rapid, reliable, non-invasive and continuous measurement of personal exposure to psychosocial stress in real-time, as experienced by individuals in their natural environments. In this interdisciplinary project, we propose to develop a new test called InstaCortisol(tm) that can be used to reliably, selectively and rapidly quantify personal exposures to stress by measuring Cortisol levels of subjects in their natural environments. Cortisol is the primary steroid produced and secreted by the adrenal cortex and is used as a reliable indicator of stress. This rapid test is based on immunoelectrochemical method where the reaction signal is measured by using cyclic voltammetry, when Cortisol sandwiches between the capture and the detector antibody, which is attached to Alkaline Phosphatase enzyme. There are four specific aims of this project. First is Sensor Fabrication: We will fabricate multiple Cortisol sensors in first two months. Second is Sensor Evaluation: we will test the sensors for sensitivity, specificity, enzyme/antibody stability, detection limit, linearity, accuracy, dynamic range, repeatability, precision, accuracy, interference, cross-reactivity, drift, and sensor calibration. We anticipate changing and/or optimizing fabrication methods to achieve benchmarks in each of these categories. Third is sensor performance: Establish sensor performance against commercially available Cortisol sensors. We aim to meet or exceed, given the need for continuous performance benchmarks of readily available Cortisol sensors. Fourth would be to Design integration feasibility experiments: A virtual evaluation of Cortisol sensor chip and its integration into a compact easy to use design. Relevance: The InstaCortisol(tm) test (being developed during Phase I), which will be part of StressSmart(tm) device during Phase II, will enable researchers to quantify personal exposure to psychosocial stress, as experienced by individuals in real-life situations. Such a capability will advance our understanding of how exposures to psychosocial stress interact with genetic factors in the etiology of prevalent human diseases of tremendous public health burden. PUBLIC HEALTH RELEVANCE: We are proposing to make a rapid test for detecting Cortisol, called "InstaCortisol", which will make real-time and continuous measurements of personal exposure to psychosocial stress quantitatively, selectively, objectively, reliably, and securely by detecting Cortisol in interstitial fluid (ISF). ISF will be harvested using our proprietary minimally invasive device called "LightAccess". The proposed product involves the integration of several cutting edge technologies. The product would require virtually no action on the part of the subject or patient thereby greatly reducing error associated with improper use. These innovations would provide a significantly improved method for assessing a molecule that plays an important role in many biological processes and disease states.

描述（由申请人提供）：“压力”反复暴露于社会心理压力与多种人类疾病（例如癌症，药物成瘾，心脏病，抑郁症）有关，巨大的公共健康负担。但是，我们的进度受到缺乏便携式设备的限制，该设备可以实时快速，可靠，无创和连续地测量个人对社会心理压力的暴露，就像个人在自然环境中所经历的那样。在这个跨学科项目中，我们建议开发一种称为Instacortisol（TM）的新测试，该测试可通过在自然环境中测量皮质醇的受试者水平来可靠，有选择和快速地量化个人压力。皮质醇是由肾上腺皮质产生和分泌的主要类固醇，用作可靠的胁迫指标。该快速测试基于免疫电化学方法，其中当捕获和检测器抗体之间的皮质醇三明治在碱性磷酸酶酶附加时，通过使用环状伏安法测量反应信号。该项目有四个具体目标。首先是传感器制造：我们将在开始两个月内制造多个皮质醇传感器。第二是传感器评估：我们将测试传感器的灵敏度，特异性，酶/抗体稳定性，检测极限，线性，准确性，动态范围，可重复性，精度，准确性，干扰性，交叉反应性，漂移和传感器校准。我们预计会更改和/或优化制造方法，以实现这些类别中的每个类别中的基准。第三是传感器性能：针对市售皮质醇传感器建立传感器性能。考虑到需要持续可用的皮质醇传感器的连续性能基准，我们的目标是满足或超过。第四是设计集成可行性实验：对皮质醇传感器芯片的虚拟评估及其整合到易于使用的紧凑型。相关性：Instacortisol（TM）测试（在I期中开发），这将是第二阶段的压力玛特（TM）设备的一部分，将使研究人员能够量化个人在现实生活中所经历的个人暴露于社会心理压力。这样的能力将提高我们对心理压力的暴露方式与遗传因素如何与巨大公共卫生负担的人类疾病的病因相互作用。公共卫生相关性：我们建议对检测皮质醇的快速测试，称为“ Instacortisol”，这将实时，连续地测量个人在定量，客观上，客观，可靠，可靠，可靠地检测性质溶酶体（ISF），以定量，客观，客观，可靠，可靠，可靠地检测皮质醇（ISF）来进行实时和连续测量。 ISF将使用我们专有的微创装置收获，称为“ LightAccess”。提出的产品涉及几种尖端技术的整合。该产品几乎不需要对受试者或患者的一部分行动，从而大大减少了与使用不当相关的错误。这些创新将为评估在许多生物过程和疾病状态中起重要作用的分子提供明显改进的方法。