PURINERGIC RECEPTOR RESEARCH

嘌呤能受体研究

• 批准号: 7358044
• 负责人: PAUL A INSEL
• 金额: $ 1.22万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358044
• 关键词: PURINERGIC; RECEPTOR; RESEARCH

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Epithelial cells along the collecting duct regulate free water reabsorption and acid secretion through various hormones and neurotransmitters, including atrial natriuretic peptide, bradykinin, catecholamines, miner-alocorticoids, prostanoids, and vasopressin. Under certain circumstances, P2-purinoceptor agonists (ATP, UTP, ADP, and UDP) can also regulate ion transport in these cells by activating various signaling cascades. Two types of P2-purinoceptors have been described to date: P2X-purinoceptors that correspond to ligand-gated cation channels and P2Y-purinoceptors that are coupled to heterotrimeric G proteins. Both the P2X- and P2Y-purinoceptors are known to occur in several isoforms, most of which exhibit wide tissue distribution. These proteins are also diffusely distributed along various nephron segments. Previous work suggests striking differences in expression of G protein coupled receptors on polarized MDCK cells but information regarding endogenously expressed purinergic P2Y receptor subtypes is not available. We are using the Bio-rad Radiance confocal microscope and Imaris software in order to examine the localization of various purinergic receptors in MDCK D-1 cell in order to detect green fluorescent protein (GFP) labeled P2Y purinergic receptors. Specifically, we are attempting to image sagital and transverse images of confluent cell layers.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。集合管上皮细胞通过各种激素和神经递质（包括心房钠尿肽、缓激肽、儿茶酚胺、矿皮质激素、前列腺素类和加压素）调节自由水重吸收和酸分泌。在某些情况下，P2-嘌呤受体激动剂（ATP、UTP、ADP 和 UDP）还可以通过激活各种信号级联来调节这些细胞中的离子转运。迄今为止，已描述了两种类型的 P2-嘌呤受体：对应于配体门控阳离子通道的 P2X-嘌呤受体和与异三聚体 G 蛋白偶联的 P2Y-嘌呤受体。已知 P2X 和 P2Y 嘌呤受体均以多种亚型存在，其中大多数表现出广泛的组织分布。这些蛋白质也沿着各个肾单位节段广泛分布。先前的研究表明极化 MDCK 细胞上 G 蛋白偶联受体的表达存在显着差异，但尚未获得有关内源表达的嘌呤能 P2Y 受体亚型的信息。我们使用 Bio-rad Radiance 共焦显微镜和 Imaris 软件来检查 MDCK D-1 细胞中各种嘌呤能受体的定位，从而检测绿色荧光蛋白 (GFP) 标记的 P2Y 嘌呤能受体。具体来说，我们正在尝试对汇合细胞层的矢状和横向图像进行成像。