Using records of large mammal populations in the Urals to decipher the timing and effects of global climate change.

利用乌拉尔地区大型哺乳动物种群的记录来破译全球气候变化的时间和影响。

• 批准号: NE/C514766/1
• 负责人: Beth Shapiro
• 金额: $ 3.94万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FC514766%2F1
• 关键词: Using; records; large; mammal; populations

Current attempts to interpret the effects of global climate change hinge on the analysis of ice and sediment cores from places such as Greenland, Antarctica and the North Atlantic. However, while these records are very detailed and go back more than 750,000 years into the past, they are effectively a proxy record of the temperature of the sea surface in surrounding areas. Consequently, it is difficult to move from sea surface temperatures to an understanding of how land animals and plants are affected by the changes apparent in such records. Ancient DNA recovered from bones of large animals from the Late Pleistocene (c. 180-12,000 years ago) provides a completely new way to obtain detailed genetic records of how populations have changed through time (eg by becoming extinct, being replaced, or migrating elsewhere) as environmental conditions change. The Ural mountains happen to have an excellent set of caves at relatively high altitude, which contain thousands of well-preserved bones of the animals who occupied the area through the Late Pleistocene. We have shown that it is possible to extract DNA from these bones, and by carbon dating them it is possible to date the DNA sequences and work out when the population disruptions occur. By studying several different species (in this case horse, caribou, wolf and brown bear) we can survey broadly across the ecosystem and look for times when many species undergo major alteration in their genetic structure. This would suggest a major (ecosystem wide) event, and a prime candidate for the cause would be climate change. Our preliminary data suggests that major alterations can be seen at the same time in animal populations as far apart as Europe and Alaska/Canada, suggesting that planet-wide (or at least hemisphere-wide) events are responsible. While the animal DNA allows us to identify when things change, and to what extent populations move around or go extinct, it doesn't necessarily identify the cause of such agitation. To investigate this issue we will examine sediment (soil) samples from the same caves that the bones originate from. Recent work at Oxford have shown that soils contain DNA from animals and especially plants that occupied an area in the past, and can provide pictures of the range and type of vegetation at different times. Cave sediments are particularly useful for this form of study because they often have good stratigraphy (steady progression of younger to older soils down through the ground), and are preserved in a relatively intact state. By examining the vegetation records and comparing them to the animal populations, we should be able to identify what plant changes are occurring in periods of major upset, and perhaps why certain species are becoming extinct while others survive. We will then relate the changes in vegetation and animal populations to the physical ice and sediment records, to try and identify how signals in these records can be related to effects on the ground. It is already very interesting that several animal extinctions seem to relate to climatic fluctuations known as Heinrich events, when fresh water sheets on the Atlantic appear to alter current flows. It is possible that short sharp climatic alterations on land may not be readily apparent in the physical records, and it will be necessary to modify the way we interpret them. This information is critical for a better understanding of past climatic change, and in order to predict the future effects of current trends such as global warming.

目前解释全球气候变化影响的尝试取决于对格陵兰岛、南极洲和北大西洋等地的冰和沉积物岩心的分析。然而，虽然这些记录非常详细并且可以追溯到 75 万多年前，但它们实际上是周边地区海面温度的代理记录。因此，很难从海洋表面温度转向了解陆地动物和植物如何受到此类记录中明显变化的影响。从晚更新世（约 180-12,000 年前）大型动物骨骼中回收的古代 DNA 提供了一种全新的方法来获取种群随时间变化的详细遗传记录（例如灭绝、被取代或迁移到其他地方） ）随着环境条件的变化。乌拉尔山脉恰好在相对较高的海拔处拥有一组绝佳的洞穴，其中包含数千个保存完好的晚更新世动物骨骼。我们已经证明，可以从这些骨头中提取 DNA，通过碳测年，可以确定 DNA 序列的年代，并确定种群扰乱何时发生。通过研究几种不同的物种（在本例中为马、驯鹿、狼和棕熊），我们可以广泛调查整个生态系统，并寻找许多物种的遗传结构发生重大改变的时期。这表明发生了重大（生态系统范围内）事件，而其原因的主要候选者就是气候变化。我们的初步数据表明，在远至欧洲和阿拉斯加/加拿大的动物种群中，可以同时看到重大变化，这表明全球范围（或至少是半球范围）的事件是造成这一现象的原因。虽然动物 DNA 可以让我们识别事物何时发生变化，以及种群移动或灭绝的程度，但它不一定能识别出这种骚动的原因。为了研究这个问题，我们将检查来自骨头来源同一洞穴的沉积物（土壤）样本。牛津大学最近的研究表明，土壤中含有来自动物、尤其是过去占据某个地区的植物的 DNA，并且可以提供不同时期植被范围和类型的图片。洞穴沉积物对于这种形式的研究特别有用，因为它们通常具有良好的地层学（从较年轻的土壤到较古老的土壤在地下的稳定进展），并且以相对完整的状态保存。通过检查植被记录并将其与动物种群进行比较，我们应该能够确定在重大动荡时期发生的植物变化，以及为什么某些物种正在灭绝而其他物种却幸存下来。然后，我们将把植被和动物种群的变化与物理冰和沉积物记录联系起来，尝试确定这些记录中的信号如何与地面影响相关。非常有趣的是，一些动物的灭绝似乎与被称为海因里希事件的气候波动有关，当时大西洋上的淡水层似乎改变了水流。陆地上短暂的剧烈气候变化可能在物理记录中并不明显，因此有必要修改我们解释它们的方式。这些信息对于更好地了解过去的气候变化以及预测全球变暖等当前趋势的未来影响至关重要。

项目成果

Is amino acid racemization a useful tool for screening for ancient DNA in bone?

氨基酸外消旋化是筛选骨骼中古代 DNA 的有用工具吗？

• DOI: http://dx.10.1098/rspb.2009.0563
• 发表时间: 2009
• 期刊: Proceedings. Biological sciences
• 作者: Collins MJ

Bayesian estimation of sequence damage in ancient DNA.

古代 DNA 序列损伤的贝叶斯估计。

• DOI: http://dx.10.1093/molbev/msm062
• 发表时间: 2007
• 期刊: Molecular biology and evolution
• 影响因子: 10.7
• 作者: Ho SY