COMPUTATIONAL MODELS OF CELL MOTILITY

细胞运动的计算模型

• 批准号: 7956388
• 负责人: ALEXANDER MOGILNER
• 金额: $ 8.14万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956388
• 关键词: 3-Dimensional; Accounting; Actin-Binding Protein; Actins; Adhesives; Biochemical; Biological; Biophysics; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Computer Simulation; Computer software; Cytoplasm; Diffusion; Elements; Environment; Equation; Funding; Grant; Imagery; Institution; Ions; Mechanics; Modeling; Molecular; Movement; Operative Surgical Procedures; Process; Reaction; Research; Research Infrastructure; Research Personnel; Resources; Signal Transduction; Simulate; Source; Specific qualifier value; Structure; Surface; System; Techniques; United States National Institutes of Health; cell motility; chemical kinetics; monomer; simulation; virtual

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cell migration is a superb example of biological complexity, as it intertwines biochemical signaling networks with biophysical locomotory processes. While the myriad of molecular components and interactions continue to become identified, the challenge looms to integrate them all into the operation of cell migration as a dynamical system. We are using the Virtual Cell (VC) environment to enable simulations of the locomotory process. The VC is already able to simulate reaction-diffusion equations on the 3-D domains (cellular interior) of complex geometries. Thus, numerical simulation and visualization of a sub-model are being developed that incorporate spatio-temporal dynamics of essential regulatory molecules in the cytoplasm. This includes reaction-diffusion equations describing chemical kinetics, diffusion and transport of actin monomers, actin binding proteins and ions. As the next step, we are enabling VC to solve the reaction-advection-diffusion equations of cytoskeletal mechanics and adhesive system on the 3-D domains and their boundaries, respectively. In addition to incorporating the appropriate numerics infrastructure to deal with the new mathematical formalisms, a key challenge will be to develop graphical representations of the biophysics that can be deployed by the user to fully specify models within a mechanics-enabled problem domain. Such representations would be structured in terms of easily manipulatable sets of components consisting of the structures, molecules, and relevant interactions. Finally, we will expand the VC software in order to dynamically change the cellular geometry to account for the protrusion/retraction movements of the cellular surface. We will adapt finite element techniques to problems of cytoskeletal dynamics with changing geometries.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 细胞迁移是生物学复杂性的一个极好的例子，因为它与生物物理运动过程将生化信号网络交织在一起。尽管无数的分子成分和相互作用继续被确定，但将它们全部整合到细胞迁移作为动力学系统的运行中的挑战隐约可见。我们正在使用虚拟单元（VC）环境来启用运动过程的模拟。 VC已经能够模拟复杂几何形状的3-D结构域（细胞内部）上的反应扩散方程。因此，正在开发出亚模型的数值模拟和可视化，以结合细胞质中基本调节分子的时空动力学。这包括描述化学动力学，肌动蛋白单体，肌动蛋白结合蛋白和离子的反应扩散方程。作为下一步，我们正在使VC分别解决3-D结构域及其边界上细胞骨架力学和粘合系统的反应 - 添加扩散方程。除了合并适当的数字基础架构以处理新的数学形式主义外，关键挑战还将是开发用户可以部署生物物理学的图形表示，以在启用机械的问题域中完全指定模型。这种表示将以易于操作的组件集组成的组成部分结构，这些组件由结构，分子和相关相互作用组成。最后，我们将扩展VC软件，以动态更改细胞几何形状，以说明细胞表面的突起/缩回运动。我们将适应有限元技术，以随着几何形状而变化的细胞骨架动力学问题。